Responses of nitrogen removal under microplastics versus nanoplastics stress in SBR: Toxicity, microbial community and functional genes

Microplastics (MPs) and nanoplastics (NPs), as emerging pollutants, are frequently detected in wastewater treatment plants. However, studies comparing the effects of MPs versus NPs on nitrogen removal by activated sludge are rarely reported. Here, the responses of nitrogen removal performance, microbial community and functional genes to MPs and NPs in sequencing batch reactors were investigated. Results revealed that MPs (10 and 1000 μg/L) had no effects on nitrogen removal. While upon exposure to NPs, although low concentration (10 μg/L) of NPs showed no remarkable influence on nitrogen removal, high level (1000 μg/L) of NPs decreased NH4+-N removal efficiency by 24.48% and caused accumulation of NO3−-N and NO2−-N. These inhibitory probably due to the acute toxicity of NPs to activated sludge, which was reflected by the increasing reactive oxygen species generation and lactate dehydrogenase release. The toxic effects of NPs further declined the relative abundance of nitrifiers (e.g., Nitrospira) and denitrifiers (e.g., Dechloromonas). These negative effects, accompanied by a decrease in abundance of amoA and nxrA genes related to nitrification (30.01% and 65.24% of control) and narG, nirK and nirS genes associated with denitrification (78.59%, 61.39%, and 86.17% of control), directly illustrated the attenuate phenomenon observed in nitrogen removal. [Display omitted] •The effects of MPs and NPs on nitrogen removal of activated sludge were compared.•Nitrogen removal was hindered by NPs but not affected by MPs.•NPs caused acute toxicity to activated sludge confirmed by ROS and LDH test.•NPs decreased the abundance of nitrifiers and denitrifiers in activated sludge.•Nitrification and denitrification genes were suppressed by NPs.