Development of a new automated IL-6 immunoassay

Quantitative detection of interleukin-6 (IL-6) in serum and plasma can help monitor immune responses and the development of acute inflammation to guide patient management. We developed an IL-6 immunoassay for use with the automated ARCHITECT system for detecting an increase in the inflammatory respo...

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Veröffentlicht in:Journal of immunological methods 2022-05, Vol.504, p.113262-113262, Article 113262
Hauptverfasser: Israeli, Eitan, Okura, Hideaki, Kreutz, Barry, Piktel, Ryan, Hadji, Abbas, Tu, Bailin, Lin, Zhihong, Hawksworth, David J., Tieman, Bryan C., Strobel, Carolyn J., Ziemann, Robert, Leary, Thomas P., Muerhoff, A. Scott, Hemken, Philip M.
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Sprache:eng
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Zusammenfassung:Quantitative detection of interleukin-6 (IL-6) in serum and plasma can help monitor immune responses and the development of acute inflammation to guide patient management. We developed an IL-6 immunoassay for use with the automated ARCHITECT system for detecting an increase in the inflammatory response. Immunized mouse sera were tested and selected B-cells were harvested for fusion with myeloma cells. A panel of monoclonal antibodies were produced, from which capture and detection monoclonal antibodies for the prototype IL-6 immunoassay were selected and screened on the ARCHITECT instrument. The antibody pair that most effectively captured and detected IL-6 was selected to develop a prototype IL-6 immunoassay. Calibrator and panel preparations using an internal recombinant IL-6 standard were compared to serum panels prepared with the IL-6 International Standard 89/548. Assay specificity and spike recovery were determined, and assay sensitivity was compared with the Roche EUA Elecsys IL-6 assay run on the cobas analyzer. Twenty-one antibodies in 441 antibody pairs were screened. The prototype IL-6 assay showed high sensitivity with an estimated limit of detection of 0.317 pg/mL and limit of quantitation of
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2022.113262