Emergence of a highly colistin-resistant Aeromonas jandaei clinical isolate harbouring four genes encoding phosphoethanolamine transferases in Nepal
This study aimed to describe a clinical isolate of Aeromonas jandaei (A. jandaei) in Nepal that harboured four types of genes encoding phosphoethanolamine transferases. An isolate of colistin-resistant A. jandaei was obtained from a blood sample of an inpatient in a hospital in Nepal, and its comple...
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| Veröffentlicht in: | International journal of antimicrobial agents 2022-04, Vol.59 (4), p.106544-106544, Article 106544 |
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| creator | Komeda, Tomoki Shrestha, Shovita Sherchan, Jatan B. Tohya, Mari Hishinuma, Tomomi Sherchand, Jeevan B. Tada, Tatsuya Kirikae, Teruo |
| description | This study aimed to describe a clinical isolate of Aeromonas jandaei (A. jandaei) in Nepal that harboured four types of genes encoding phosphoethanolamine transferases.
An isolate of colistin-resistant A. jandaei was obtained from a blood sample of an inpatient in a hospital in Nepal, and its complete genome sequence was determined. Escherichia coli (E. coli) and Aeromonas hydrophila (A. hydrophila) transformants expressing genes encoding novel phosphoethanolamine transferase variants were constructed and colistin-susceptibility profiles were determined.
The isolate harboured four genes encoding phosphoethanolamine transferases on the chromosome, which were designated eptAv3.2, eptAv3.3, eptAv3.4 and eptAv7.2. The amino acid sequences of EptAv3.2, 3.3 and 3.4 were > 80% identical to MCR-3.1, and that of EptAv7.2 was > 79% identical to MCR-7.1. E. coli expressing eptAv3.2, 3.3 and 3.4 showed reduced susceptibility to colistin, whereas E. coli expressing eptAv7.2 did not. In contrast, A. hydrophila expressing eptAv7.2 showed reduced susceptibility to colistin, whereas A. hydrophila expressing eptAv3.2, 3.3 and 3.4 did not; eptAv3.3 and 3.4 formed a tandem structure. The genomic environments surrounding eptAv3.2, 3.3 and 3.4 were similar to Aeromonas veronii obtained from the effluent of a treatment plant in Japan in 2018. The genomic environment surrounding eptAv7.2 was similar to that of A. jandaei obtained from a chicken in the USA in 2019.
The highly colistin-resistant A. jandaei clinical isolate harboured four chromosomal genes encoding phosphoethanolamine transferases, suggesting that Aeromonas spp. harbouring eptAv genes with strong similarities to mcr-3 and mcr-7 are emerging in medical settings as well as environments. |
| doi_str_mv | 10.1016/j.ijantimicag.2022.106544 |
| format | Article |
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An isolate of colistin-resistant A. jandaei was obtained from a blood sample of an inpatient in a hospital in Nepal, and its complete genome sequence was determined. Escherichia coli (E. coli) and Aeromonas hydrophila (A. hydrophila) transformants expressing genes encoding novel phosphoethanolamine transferase variants were constructed and colistin-susceptibility profiles were determined.
The isolate harboured four genes encoding phosphoethanolamine transferases on the chromosome, which were designated eptAv3.2, eptAv3.3, eptAv3.4 and eptAv7.2. The amino acid sequences of EptAv3.2, 3.3 and 3.4 were > 80% identical to MCR-3.1, and that of EptAv7.2 was > 79% identical to MCR-7.1. E. coli expressing eptAv3.2, 3.3 and 3.4 showed reduced susceptibility to colistin, whereas E. coli expressing eptAv7.2 did not. In contrast, A. hydrophila expressing eptAv7.2 showed reduced susceptibility to colistin, whereas A. hydrophila expressing eptAv3.2, 3.3 and 3.4 did not; eptAv3.3 and 3.4 formed a tandem structure. The genomic environments surrounding eptAv3.2, 3.3 and 3.4 were similar to Aeromonas veronii obtained from the effluent of a treatment plant in Japan in 2018. The genomic environment surrounding eptAv7.2 was similar to that of A. jandaei obtained from a chicken in the USA in 2019.
The highly colistin-resistant A. jandaei clinical isolate harboured four chromosomal genes encoding phosphoethanolamine transferases, suggesting that Aeromonas spp. harbouring eptAv genes with strong similarities to mcr-3 and mcr-7 are emerging in medical settings as well as environments.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2022.106544</identifier><identifier>PMID: 35134503</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Aeromonas - genetics ; Aeromonas hydrophila ; Aeromonas jandaei ; Aeromonas veronii ; amino acids ; Anti-Bacterial Agents - pharmacology ; blood sampling ; chickens ; chromosomes ; colistin ; Colistin - pharmacology ; Colistin resistance ; Drug Resistance, Bacterial - genetics ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli Proteins - genetics ; Ethanolaminephosphotransferase - genetics ; Ethanolaminephosphotransferase - metabolism ; Ethanolamines ; genomics ; hospitals ; Japan ; Microbial Sensitivity Tests ; Nepal ; nucleotide sequences ; Phosphoethanolamine transferases ; Plasmids ; transferases</subject><ispartof>International journal of antimicrobial agents, 2022-04, Vol.59 (4), p.106544-106544, Article 106544</ispartof><rights>2022 The Author(s)</rights><rights>Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-ce8b91df311c72f85586b4b6a03a59e8b1145d12ae242674567f10e538ac74893</citedby><cites>FETCH-LOGICAL-c461t-ce8b91df311c72f85586b4b6a03a59e8b1145d12ae242674567f10e538ac74893</cites><orcidid>0000-0001-9427-3154</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijantimicag.2022.106544$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35134503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komeda, Tomoki</creatorcontrib><creatorcontrib>Shrestha, Shovita</creatorcontrib><creatorcontrib>Sherchan, Jatan B.</creatorcontrib><creatorcontrib>Tohya, Mari</creatorcontrib><creatorcontrib>Hishinuma, Tomomi</creatorcontrib><creatorcontrib>Sherchand, Jeevan B.</creatorcontrib><creatorcontrib>Tada, Tatsuya</creatorcontrib><creatorcontrib>Kirikae, Teruo</creatorcontrib><title>Emergence of a highly colistin-resistant Aeromonas jandaei clinical isolate harbouring four genes encoding phosphoethanolamine transferases in Nepal</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>This study aimed to describe a clinical isolate of Aeromonas jandaei (A. jandaei) in Nepal that harboured four types of genes encoding phosphoethanolamine transferases.
An isolate of colistin-resistant A. jandaei was obtained from a blood sample of an inpatient in a hospital in Nepal, and its complete genome sequence was determined. Escherichia coli (E. coli) and Aeromonas hydrophila (A. hydrophila) transformants expressing genes encoding novel phosphoethanolamine transferase variants were constructed and colistin-susceptibility profiles were determined.
The isolate harboured four genes encoding phosphoethanolamine transferases on the chromosome, which were designated eptAv3.2, eptAv3.3, eptAv3.4 and eptAv7.2. The amino acid sequences of EptAv3.2, 3.3 and 3.4 were > 80% identical to MCR-3.1, and that of EptAv7.2 was > 79% identical to MCR-7.1. E. coli expressing eptAv3.2, 3.3 and 3.4 showed reduced susceptibility to colistin, whereas E. coli expressing eptAv7.2 did not. In contrast, A. hydrophila expressing eptAv7.2 showed reduced susceptibility to colistin, whereas A. hydrophila expressing eptAv3.2, 3.3 and 3.4 did not; eptAv3.3 and 3.4 formed a tandem structure. The genomic environments surrounding eptAv3.2, 3.3 and 3.4 were similar to Aeromonas veronii obtained from the effluent of a treatment plant in Japan in 2018. The genomic environment surrounding eptAv7.2 was similar to that of A. jandaei obtained from a chicken in the USA in 2019.
The highly colistin-resistant A. jandaei clinical isolate harboured four chromosomal genes encoding phosphoethanolamine transferases, suggesting that Aeromonas spp. harbouring eptAv genes with strong similarities to mcr-3 and mcr-7 are emerging in medical settings as well as environments.</description><subject>Aeromonas - genetics</subject><subject>Aeromonas hydrophila</subject><subject>Aeromonas jandaei</subject><subject>Aeromonas veronii</subject><subject>amino acids</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>blood sampling</subject><subject>chickens</subject><subject>chromosomes</subject><subject>colistin</subject><subject>Colistin - pharmacology</subject><subject>Colistin resistance</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Ethanolaminephosphotransferase - genetics</subject><subject>Ethanolaminephosphotransferase - metabolism</subject><subject>Ethanolamines</subject><subject>genomics</subject><subject>hospitals</subject><subject>Japan</subject><subject>Microbial Sensitivity Tests</subject><subject>Nepal</subject><subject>nucleotide sequences</subject><subject>Phosphoethanolamine transferases</subject><subject>Plasmids</subject><subject>transferases</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcuO1DAQtBCIHQZ-AZkblwx-JzmuRstDWsEFzpbjdCYeOfZgZ5D2P_hgejQL4rgHq1vt6qpWFSHvONtxxs2H4y4cXVrDErw77AQTAudGK_WMbHjXiqbtuXxONqwXqul029-QV7UeGeNaKv2S3EjNsWFyQ37fLVAOkDzQPFFH53CY4wP1OYa6htQUqNigGL2FkpecXKWoPToI1MeQ8IJIQ83RrUBnV4Z8LiEd6ISVIi9UiuR5vMxOc674YJ1dwoUlJKBrcalOUFxFZEj0K5xcfE1eTC5WePNYt-THx7vv-8_N_bdPX_a3941Xhq-Nh27o-ThJzn0rpk7rzgxqMI5Jp3v85FzpkQsHQgnTKm3aiTPQsnO-VV0vt-T9lfdU8s8z1NUuoXqI0SXI52qFUV1n0DTxBKho0VOJpm5Jf4X6kmstMNlTCYsrD5Yze8nPHu1_-dlLfvaaH-6-fZQ5DwuM_zb_BoaA_RUA6MuvAMVWHy7xjaGAX-2YwxNk_gDzYrSq</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Komeda, Tomoki</creator><creator>Shrestha, Shovita</creator><creator>Sherchan, Jatan B.</creator><creator>Tohya, Mari</creator><creator>Hishinuma, Tomomi</creator><creator>Sherchand, Jeevan B.</creator><creator>Tada, Tatsuya</creator><creator>Kirikae, Teruo</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9427-3154</orcidid></search><sort><creationdate>202204</creationdate><title>Emergence of a highly colistin-resistant Aeromonas jandaei clinical isolate harbouring four genes encoding phosphoethanolamine transferases in Nepal</title><author>Komeda, Tomoki ; Shrestha, Shovita ; Sherchan, Jatan B. ; Tohya, Mari ; Hishinuma, Tomomi ; Sherchand, Jeevan B. ; Tada, Tatsuya ; Kirikae, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-ce8b91df311c72f85586b4b6a03a59e8b1145d12ae242674567f10e538ac74893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aeromonas - genetics</topic><topic>Aeromonas hydrophila</topic><topic>Aeromonas jandaei</topic><topic>Aeromonas veronii</topic><topic>amino acids</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>blood sampling</topic><topic>chickens</topic><topic>chromosomes</topic><topic>colistin</topic><topic>Colistin - pharmacology</topic><topic>Colistin resistance</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Ethanolaminephosphotransferase - genetics</topic><topic>Ethanolaminephosphotransferase - metabolism</topic><topic>Ethanolamines</topic><topic>genomics</topic><topic>hospitals</topic><topic>Japan</topic><topic>Microbial Sensitivity Tests</topic><topic>Nepal</topic><topic>nucleotide sequences</topic><topic>Phosphoethanolamine transferases</topic><topic>Plasmids</topic><topic>transferases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Komeda, Tomoki</creatorcontrib><creatorcontrib>Shrestha, Shovita</creatorcontrib><creatorcontrib>Sherchan, Jatan B.</creatorcontrib><creatorcontrib>Tohya, Mari</creatorcontrib><creatorcontrib>Hishinuma, Tomomi</creatorcontrib><creatorcontrib>Sherchand, Jeevan B.</creatorcontrib><creatorcontrib>Tada, Tatsuya</creatorcontrib><creatorcontrib>Kirikae, Teruo</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Komeda, Tomoki</au><au>Shrestha, Shovita</au><au>Sherchan, Jatan B.</au><au>Tohya, Mari</au><au>Hishinuma, Tomomi</au><au>Sherchand, Jeevan B.</au><au>Tada, Tatsuya</au><au>Kirikae, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emergence of a highly colistin-resistant Aeromonas jandaei clinical isolate harbouring four genes encoding phosphoethanolamine transferases in Nepal</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2022-04</date><risdate>2022</risdate><volume>59</volume><issue>4</issue><spage>106544</spage><epage>106544</epage><pages>106544-106544</pages><artnum>106544</artnum><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>This study aimed to describe a clinical isolate of Aeromonas jandaei (A. jandaei) in Nepal that harboured four types of genes encoding phosphoethanolamine transferases.
An isolate of colistin-resistant A. jandaei was obtained from a blood sample of an inpatient in a hospital in Nepal, and its complete genome sequence was determined. Escherichia coli (E. coli) and Aeromonas hydrophila (A. hydrophila) transformants expressing genes encoding novel phosphoethanolamine transferase variants were constructed and colistin-susceptibility profiles were determined.
The isolate harboured four genes encoding phosphoethanolamine transferases on the chromosome, which were designated eptAv3.2, eptAv3.3, eptAv3.4 and eptAv7.2. The amino acid sequences of EptAv3.2, 3.3 and 3.4 were > 80% identical to MCR-3.1, and that of EptAv7.2 was > 79% identical to MCR-7.1. E. coli expressing eptAv3.2, 3.3 and 3.4 showed reduced susceptibility to colistin, whereas E. coli expressing eptAv7.2 did not. In contrast, A. hydrophila expressing eptAv7.2 showed reduced susceptibility to colistin, whereas A. hydrophila expressing eptAv3.2, 3.3 and 3.4 did not; eptAv3.3 and 3.4 formed a tandem structure. The genomic environments surrounding eptAv3.2, 3.3 and 3.4 were similar to Aeromonas veronii obtained from the effluent of a treatment plant in Japan in 2018. The genomic environment surrounding eptAv7.2 was similar to that of A. jandaei obtained from a chicken in the USA in 2019.
The highly colistin-resistant A. jandaei clinical isolate harboured four chromosomal genes encoding phosphoethanolamine transferases, suggesting that Aeromonas spp. harbouring eptAv genes with strong similarities to mcr-3 and mcr-7 are emerging in medical settings as well as environments.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>35134503</pmid><doi>10.1016/j.ijantimicag.2022.106544</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9427-3154</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of antimicrobial agents, 2022-04, Vol.59 (4), p.106544-106544, Article 106544 |
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| subjects | Aeromonas - genetics Aeromonas hydrophila Aeromonas jandaei Aeromonas veronii amino acids Anti-Bacterial Agents - pharmacology blood sampling chickens chromosomes colistin Colistin - pharmacology Colistin resistance Drug Resistance, Bacterial - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - genetics Ethanolaminephosphotransferase - genetics Ethanolaminephosphotransferase - metabolism Ethanolamines genomics hospitals Japan Microbial Sensitivity Tests Nepal nucleotide sequences Phosphoethanolamine transferases Plasmids transferases |
| title | Emergence of a highly colistin-resistant Aeromonas jandaei clinical isolate harbouring four genes encoding phosphoethanolamine transferases in Nepal |
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