Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry

Converting discrete microbial metabolites into chemical probes for chemical biology and medicinal chemistry studies is typically preceded by lengthy purification and chemical derivatization processes. Standard practice involves purifying the target microbial metabolite from culture, followed by deri...

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Veröffentlicht in:	Methods in enzymology 2022, Vol.665, p.49-71
Hauptverfasser:	Gotsbacher, Michael P, Codd, Rachel
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkynes - chemistry Azides - chemistry Click Chemistry - methods Cycloaddition Reaction Indicators and Reagents
Online-Zugang:	Volltext
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