A split prime editor with untethered reverse transcriptase and circular RNA template

Delivery and optimization of prime editors (PEs) have been hampered by their large size and complexity. Although split versions of genome-editing tools can reduce construct size, they require special engineering to tether the binding and catalytic domains. Here we report a split PE (sPE) in which th...

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Veröffentlicht in:	Nature biotechnology 2022-09, Vol.40 (9), p.1388-1393
Hauptverfasser:	Liu, Bin, Dong, Xiaolong, Cheng, Haoyang, Zheng, Chunwei, Chen, Zexiang, Rodríguez, Tomás C., Liang, Shun-Qing, Xue, Wen, Sontheimer, Erik J.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1511 692/308/2056 Agriculture Animals Binding sites Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Circular RNA Complexity CRISPR-Cas Systems Deoxyribonuclease I - genetics Editing Efficiency Gene Editing Genome editing Genomes Insertion Inteins Life Sciences Medical schools Mice Modular construction Modular systems Mutation Nuclease Optimization Plasmids Protein structure Proteins Ribonucleic acid RNA RNA, Circular - genetics RNA, Guide, CRISPR-Cas Systems RNA-directed DNA polymerase RNA-Directed DNA Polymerase - genetics Tumors Tyrosinemias - genetics β-Catenin
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