Comparative performance of five recombinant and chimeric antigens in a time-resolved fluorescence immunoassay for detection of Toxoplasma gondii infection in cats
Felids are definitive hosts of Toxoplasma gondii, being the only hosts that can spread the infection through oocyst shedding in their feces. The elevated presence of this parasite in the domestic cat (Felis catus), and its close contact with humans, make it necessary to obtain reliable diagnostic me...
Gespeichert in:
| Veröffentlicht in: | Veterinary parasitology 2022-04, Vol.304, p.109703-109703, Article 109703 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 109703 |
|---|---|
| container_issue | |
| container_start_page | 109703 |
| container_title | Veterinary parasitology |
| container_volume | 304 |
| creator | Huertas-López, Ana Contreras Rojo, Marinela Sukhumavasi, Woraporn Martínez-Subiela, Silvia Álvarez-García, Gema López-Ureña, Nadia María Cerón, José Joaquín Martínez-Carrasco, Carlos |
| description | Felids are definitive hosts of Toxoplasma gondii, being the only hosts that can spread the infection through oocyst shedding in their feces. The elevated presence of this parasite in the domestic cat (Felis catus), and its close contact with humans, make it necessary to obtain reliable diagnostic methods to detect positive animals as a public health measure. For this reason, in this study, the diagnostic performance of five different recombinant antigen-based techniques was assessed to diagnose T. gondii infection in cat blood plasma samples. Specifically, four T. gondii recombinant antigens (GRA7, truncated GRA7, SAG2, and truncated SAG2) and a chimeric antigen (SAG1-GRA8) were used. A time-resolved fluorescence immunoassay (TRFIA) was developed for each antigen, and the results of each of these techniques were compared with those obtained by a commercial enzyme-linked immunoassay (ELISA) and a modified agglutination test (MAT) as reference techniques. The TRFIA based on SAG1-GRA8 antigen showed better discrimination between seropositive and seronegative cats (p |
| doi_str_mv | 10.1016/j.vetpar.2022.109703 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2646941661</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304401722000577</els_id><sourcerecordid>2646941661</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-d9c6da3472da26f1d2a9f7e68baccb0adf228982e42c379f35ec903db5b953d13</originalsourceid><addsrcrecordid>eNp9kc-OFCEQxonRuOPqGxjD0UuPNHTT0xcTM_FfsomX9UyqoViZNDACPXFfxyeVTo8ePZGq-ur3pfgIed2yfcta-e60v2A5Q9pzxnltjQMTT8iuPQyi4X3PnpIdE6xrOtYON-RFzifGWMfk8JzciF7IYWTdjvw-Rl8hUNwF6RmTjclD0EijpXbtJdTRTy5AKBSCofqH85icrkVxDxgydYECLbXbJMxxvqChdl5iLTSuJOf9EiLkDI-04qnBgrq4GFaP-_grnmfIHuhDDMa5irPXcQVrKPkleWZhzvjq-t6S758-3h-_NHffPn89frhrtOiH0phRSwOiG7gBLm1rOIx2QHmYQOuJgbGcH8YDx45rMYxW9KhHJszUT2MvTCtuyduNe07x54K5KO_qCfMMAeOSFZedHLtWylXabVKdYs4JrTon5yE9qpapNR11Uls6ak1HbenUtTdXh2XyaP4t_Y2jCt5vAqx3XhwmlbVbP9G4mkNRJrr_O_wBOT-nlQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2646941661</pqid></control><display><type>article</type><title>Comparative performance of five recombinant and chimeric antigens in a time-resolved fluorescence immunoassay for detection of Toxoplasma gondii infection in cats</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Huertas-López, Ana ; Contreras Rojo, Marinela ; Sukhumavasi, Woraporn ; Martínez-Subiela, Silvia ; Álvarez-García, Gema ; López-Ureña, Nadia María ; Cerón, José Joaquín ; Martínez-Carrasco, Carlos</creator><creatorcontrib>Huertas-López, Ana ; Contreras Rojo, Marinela ; Sukhumavasi, Woraporn ; Martínez-Subiela, Silvia ; Álvarez-García, Gema ; López-Ureña, Nadia María ; Cerón, José Joaquín ; Martínez-Carrasco, Carlos</creatorcontrib><description>Felids are definitive hosts of Toxoplasma gondii, being the only hosts that can spread the infection through oocyst shedding in their feces. The elevated presence of this parasite in the domestic cat (Felis catus), and its close contact with humans, make it necessary to obtain reliable diagnostic methods to detect positive animals as a public health measure. For this reason, in this study, the diagnostic performance of five different recombinant antigen-based techniques was assessed to diagnose T. gondii infection in cat blood plasma samples. Specifically, four T. gondii recombinant antigens (GRA7, truncated GRA7, SAG2, and truncated SAG2) and a chimeric antigen (SAG1-GRA8) were used. A time-resolved fluorescence immunoassay (TRFIA) was developed for each antigen, and the results of each of these techniques were compared with those obtained by a commercial enzyme-linked immunoassay (ELISA) and a modified agglutination test (MAT) as reference techniques. The TRFIA based on SAG1-GRA8 antigen showed better discrimination between seropositive and seronegative cats (p < 0.001), as well as a better area under the curve (0.95), sensitivity (93.6%), and specificity (89.5%) values for the optimal cut-off, versus the other TRFIAs. In addition, SAG1-GRA8 TRFIA showed substantial agreement (kappa value = 0.78) and a moderate significant correlation (Spearman’s correlation: r = 0.62, p < 0.001) compared with the reference techniques. On the other hand, since plasma samples were obtained from 101 cats in Bangkok city and four of them were Neospora caninum seropositive by indirect immunofluorescence assay (IFAT), this is the first time that anti-N. caninum antibodies are detected in cats in Thailand. In conclusion, our study highlights that the TRFIA with TgSAG1-GRA8 antigen is an accurate and recommended diagnostic technique for detecting anti-T. gondii antibodies in cats.
[Display omitted]
•Five antigens were tested to detect anti-T. gondii IgG in feline plasma by TRFIA.•TRFIA based on the SAG1-GRA8 antigen showed the best diagnostic performance.•SAG1-GRA8 based TRFIA is accurate for serodiagnosis of T. gondii infection in cats.•This is also the first report of Neospora caninum seropositive cats in Thailand.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2022.109703</identifier><identifier>PMID: 35367904</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Protozoan ; Antigens, Protozoan ; Cat Diseases - diagnosis ; Cats ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; GRA7 ; SAG1-GRA8 ; SAG2 ; Thailand ; Time-resolved fluorescence immunoassay ; Toxoplasma ; Toxoplasma gondii ; Toxoplasmosis, Animal - diagnosis ; Toxoplasmosis, Animal - parasitology</subject><ispartof>Veterinary parasitology, 2022-04, Vol.304, p.109703-109703, Article 109703</ispartof><rights>2022 The Authors</rights><rights>Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c357t-d9c6da3472da26f1d2a9f7e68baccb0adf228982e42c379f35ec903db5b953d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2022.109703$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35367904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huertas-López, Ana</creatorcontrib><creatorcontrib>Contreras Rojo, Marinela</creatorcontrib><creatorcontrib>Sukhumavasi, Woraporn</creatorcontrib><creatorcontrib>Martínez-Subiela, Silvia</creatorcontrib><creatorcontrib>Álvarez-García, Gema</creatorcontrib><creatorcontrib>López-Ureña, Nadia María</creatorcontrib><creatorcontrib>Cerón, José Joaquín</creatorcontrib><creatorcontrib>Martínez-Carrasco, Carlos</creatorcontrib><title>Comparative performance of five recombinant and chimeric antigens in a time-resolved fluorescence immunoassay for detection of Toxoplasma gondii infection in cats</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Felids are definitive hosts of Toxoplasma gondii, being the only hosts that can spread the infection through oocyst shedding in their feces. The elevated presence of this parasite in the domestic cat (Felis catus), and its close contact with humans, make it necessary to obtain reliable diagnostic methods to detect positive animals as a public health measure. For this reason, in this study, the diagnostic performance of five different recombinant antigen-based techniques was assessed to diagnose T. gondii infection in cat blood plasma samples. Specifically, four T. gondii recombinant antigens (GRA7, truncated GRA7, SAG2, and truncated SAG2) and a chimeric antigen (SAG1-GRA8) were used. A time-resolved fluorescence immunoassay (TRFIA) was developed for each antigen, and the results of each of these techniques were compared with those obtained by a commercial enzyme-linked immunoassay (ELISA) and a modified agglutination test (MAT) as reference techniques. The TRFIA based on SAG1-GRA8 antigen showed better discrimination between seropositive and seronegative cats (p < 0.001), as well as a better area under the curve (0.95), sensitivity (93.6%), and specificity (89.5%) values for the optimal cut-off, versus the other TRFIAs. In addition, SAG1-GRA8 TRFIA showed substantial agreement (kappa value = 0.78) and a moderate significant correlation (Spearman’s correlation: r = 0.62, p < 0.001) compared with the reference techniques. On the other hand, since plasma samples were obtained from 101 cats in Bangkok city and four of them were Neospora caninum seropositive by indirect immunofluorescence assay (IFAT), this is the first time that anti-N. caninum antibodies are detected in cats in Thailand. In conclusion, our study highlights that the TRFIA with TgSAG1-GRA8 antigen is an accurate and recommended diagnostic technique for detecting anti-T. gondii antibodies in cats.
[Display omitted]
•Five antigens were tested to detect anti-T. gondii IgG in feline plasma by TRFIA.•TRFIA based on the SAG1-GRA8 antigen showed the best diagnostic performance.•SAG1-GRA8 based TRFIA is accurate for serodiagnosis of T. gondii infection in cats.•This is also the first report of Neospora caninum seropositive cats in Thailand.</description><subject>Animals</subject><subject>Antibodies, Protozoan</subject><subject>Antigens, Protozoan</subject><subject>Cat Diseases - diagnosis</subject><subject>Cats</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>GRA7</subject><subject>SAG1-GRA8</subject><subject>SAG2</subject><subject>Thailand</subject><subject>Time-resolved fluorescence immunoassay</subject><subject>Toxoplasma</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - diagnosis</subject><subject>Toxoplasmosis, Animal - parasitology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-OFCEQxonRuOPqGxjD0UuPNHTT0xcTM_FfsomX9UyqoViZNDACPXFfxyeVTo8ePZGq-ur3pfgIed2yfcta-e60v2A5Q9pzxnltjQMTT8iuPQyi4X3PnpIdE6xrOtYON-RFzifGWMfk8JzciF7IYWTdjvw-Rl8hUNwF6RmTjclD0EijpXbtJdTRTy5AKBSCofqH85icrkVxDxgydYECLbXbJMxxvqChdl5iLTSuJOf9EiLkDI-04qnBgrq4GFaP-_grnmfIHuhDDMa5irPXcQVrKPkleWZhzvjq-t6S758-3h-_NHffPn89frhrtOiH0phRSwOiG7gBLm1rOIx2QHmYQOuJgbGcH8YDx45rMYxW9KhHJszUT2MvTCtuyduNe07x54K5KO_qCfMMAeOSFZedHLtWylXabVKdYs4JrTon5yE9qpapNR11Uls6ak1HbenUtTdXh2XyaP4t_Y2jCt5vAqx3XhwmlbVbP9G4mkNRJrr_O_wBOT-nlQ</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Huertas-López, Ana</creator><creator>Contreras Rojo, Marinela</creator><creator>Sukhumavasi, Woraporn</creator><creator>Martínez-Subiela, Silvia</creator><creator>Álvarez-García, Gema</creator><creator>López-Ureña, Nadia María</creator><creator>Cerón, José Joaquín</creator><creator>Martínez-Carrasco, Carlos</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202204</creationdate><title>Comparative performance of five recombinant and chimeric antigens in a time-resolved fluorescence immunoassay for detection of Toxoplasma gondii infection in cats</title><author>Huertas-López, Ana ; Contreras Rojo, Marinela ; Sukhumavasi, Woraporn ; Martínez-Subiela, Silvia ; Álvarez-García, Gema ; López-Ureña, Nadia María ; Cerón, José Joaquín ; Martínez-Carrasco, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-d9c6da3472da26f1d2a9f7e68baccb0adf228982e42c379f35ec903db5b953d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antibodies, Protozoan</topic><topic>Antigens, Protozoan</topic><topic>Cat Diseases - diagnosis</topic><topic>Cats</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>GRA7</topic><topic>SAG1-GRA8</topic><topic>SAG2</topic><topic>Thailand</topic><topic>Time-resolved fluorescence immunoassay</topic><topic>Toxoplasma</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - diagnosis</topic><topic>Toxoplasmosis, Animal - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huertas-López, Ana</creatorcontrib><creatorcontrib>Contreras Rojo, Marinela</creatorcontrib><creatorcontrib>Sukhumavasi, Woraporn</creatorcontrib><creatorcontrib>Martínez-Subiela, Silvia</creatorcontrib><creatorcontrib>Álvarez-García, Gema</creatorcontrib><creatorcontrib>López-Ureña, Nadia María</creatorcontrib><creatorcontrib>Cerón, José Joaquín</creatorcontrib><creatorcontrib>Martínez-Carrasco, Carlos</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huertas-López, Ana</au><au>Contreras Rojo, Marinela</au><au>Sukhumavasi, Woraporn</au><au>Martínez-Subiela, Silvia</au><au>Álvarez-García, Gema</au><au>López-Ureña, Nadia María</au><au>Cerón, José Joaquín</au><au>Martínez-Carrasco, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative performance of five recombinant and chimeric antigens in a time-resolved fluorescence immunoassay for detection of Toxoplasma gondii infection in cats</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2022-04</date><risdate>2022</risdate><volume>304</volume><spage>109703</spage><epage>109703</epage><pages>109703-109703</pages><artnum>109703</artnum><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Felids are definitive hosts of Toxoplasma gondii, being the only hosts that can spread the infection through oocyst shedding in their feces. The elevated presence of this parasite in the domestic cat (Felis catus), and its close contact with humans, make it necessary to obtain reliable diagnostic methods to detect positive animals as a public health measure. For this reason, in this study, the diagnostic performance of five different recombinant antigen-based techniques was assessed to diagnose T. gondii infection in cat blood plasma samples. Specifically, four T. gondii recombinant antigens (GRA7, truncated GRA7, SAG2, and truncated SAG2) and a chimeric antigen (SAG1-GRA8) were used. A time-resolved fluorescence immunoassay (TRFIA) was developed for each antigen, and the results of each of these techniques were compared with those obtained by a commercial enzyme-linked immunoassay (ELISA) and a modified agglutination test (MAT) as reference techniques. The TRFIA based on SAG1-GRA8 antigen showed better discrimination between seropositive and seronegative cats (p < 0.001), as well as a better area under the curve (0.95), sensitivity (93.6%), and specificity (89.5%) values for the optimal cut-off, versus the other TRFIAs. In addition, SAG1-GRA8 TRFIA showed substantial agreement (kappa value = 0.78) and a moderate significant correlation (Spearman’s correlation: r = 0.62, p < 0.001) compared with the reference techniques. On the other hand, since plasma samples were obtained from 101 cats in Bangkok city and four of them were Neospora caninum seropositive by indirect immunofluorescence assay (IFAT), this is the first time that anti-N. caninum antibodies are detected in cats in Thailand. In conclusion, our study highlights that the TRFIA with TgSAG1-GRA8 antigen is an accurate and recommended diagnostic technique for detecting anti-T. gondii antibodies in cats.
[Display omitted]
•Five antigens were tested to detect anti-T. gondii IgG in feline plasma by TRFIA.•TRFIA based on the SAG1-GRA8 antigen showed the best diagnostic performance.•SAG1-GRA8 based TRFIA is accurate for serodiagnosis of T. gondii infection in cats.•This is also the first report of Neospora caninum seropositive cats in Thailand.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>35367904</pmid><doi>10.1016/j.vetpar.2022.109703</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2022-04, Vol.304, p.109703-109703, Article 109703 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2646941661 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies, Protozoan Antigens, Protozoan Cat Diseases - diagnosis Cats Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary GRA7 SAG1-GRA8 SAG2 Thailand Time-resolved fluorescence immunoassay Toxoplasma Toxoplasma gondii Toxoplasmosis, Animal - diagnosis Toxoplasmosis, Animal - parasitology |
| title | Comparative performance of five recombinant and chimeric antigens in a time-resolved fluorescence immunoassay for detection of Toxoplasma gondii infection in cats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T13%3A58%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20performance%20of%20five%20recombinant%20and%20chimeric%20antigens%20in%20a%20time-resolved%20fluorescence%20immunoassay%20for%20detection%20of%20Toxoplasma%20gondii%20infection%20in%20cats&rft.jtitle=Veterinary%20parasitology&rft.au=Huertas-L%C3%B3pez,%20Ana&rft.date=2022-04&rft.volume=304&rft.spage=109703&rft.epage=109703&rft.pages=109703-109703&rft.artnum=109703&rft.issn=0304-4017&rft.eissn=1873-2550&rft_id=info:doi/10.1016/j.vetpar.2022.109703&rft_dat=%3Cproquest_cross%3E2646941661%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2646941661&rft_id=info:pmid/35367904&rft_els_id=S0304401722000577&rfr_iscdi=true |