Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling
Background Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated. M...
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| Veröffentlicht in: | Molecular biology reports 2022-06, Vol.49 (6), p.4673-4681 |
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| creator | Tan, Xiao-Hui Gu, Sheng-Ji Tian, Wen-Jie Song, Wen-Peng Gu, Yang-Yang Yuan, Yi-Ming Li, Xue-Song Xin, Zhong-Cheng Kim, Sae Woong Guan, Rui-Li Bae, Woong Jin |
| description | Background
Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated.
Methods
EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs.
Results
A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia.
Conclusions
Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility. |
| doi_str_mv | 10.1007/s11033-022-07316-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2646938063</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2646938063</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-740af847051233bf81a0c0181479578f23cd1b182d95cd08fd2f5c60f6acd7b93</originalsourceid><addsrcrecordid>eNp9kU1LHTEUhkNR6q32D3RRAm5cdOw5k8kk053IrS2IQtV1yM2HRubDJjNS_fVmvLcKLlwFcp73PQkPIV8QDhFAfE-IwFgBZVmAYFgX-IEskAtWVI2QW2QBDLCoJMcd8imlWwCoUPCPZIdxVteCNwuSlv_GqI1r26nVkd67FEzrErUuhntnqY9DR2-mTvf0wsVxaAOd4fSDmhudg2PmHvUYhj59o3dxGN3QBUN1r9uHFPKd7i3twp-zo3nqQxv66z2y7XWb3OfNuUuufi4vj38Vp-cnv4-PTgvDBB8LUYH2shLAsWRs5SVqMIASK9FwIX3JjMUVytI23FiQ3paemxp8rY0Vq4btkoN1b978d3JpVF1I8-t174YpqbKu6oZJqFlG99-gt8MU8ydmSnKJknPMVLmmTBxSis6ruxg6HR8UgpqVqLUSlZWoZyVqDn3dVE-rztmXyH8HGWBrIOVRf-3i6-53ap8A9jyXWg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2685818551</pqid></control><display><type>article</type><title>Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling</title><source>SpringerLink Journals - AutoHoldings</source><creator>Tan, Xiao-Hui ; Gu, Sheng-Ji ; Tian, Wen-Jie ; Song, Wen-Peng ; Gu, Yang-Yang ; Yuan, Yi-Ming ; Li, Xue-Song ; Xin, Zhong-Cheng ; Kim, Sae Woong ; Guan, Rui-Li ; Bae, Woong Jin</creator><creatorcontrib>Tan, Xiao-Hui ; Gu, Sheng-Ji ; Tian, Wen-Jie ; Song, Wen-Peng ; Gu, Yang-Yang ; Yuan, Yi-Ming ; Li, Xue-Song ; Xin, Zhong-Cheng ; Kim, Sae Woong ; Guan, Rui-Li ; Bae, Woong Jin</creatorcontrib><description>Background
Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated.
Methods
EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs.
Results
A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia.
Conclusions
Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07316-1</identifier><identifier>PMID: 35366759</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Cell cycle ; Clusterin ; Enzymatic activity ; Extracellular vesicles ; Galectin-1 ; Histology ; Immune system ; Infertility ; Life Sciences ; Liquid chromatography ; Mass spectroscopy ; MicroRNAs ; miRNA ; Morphology ; Oligozoospermia ; Original Article ; Proteins ; Proteomics ; Sertoli cells ; Spermatogenesis ; Transmission electron microscopy ; Ultracentrifugation ; Western blotting</subject><ispartof>Molecular biology reports, 2022-06, Vol.49 (6), p.4673-4681</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-740af847051233bf81a0c0181479578f23cd1b182d95cd08fd2f5c60f6acd7b93</citedby><cites>FETCH-LOGICAL-c375t-740af847051233bf81a0c0181479578f23cd1b182d95cd08fd2f5c60f6acd7b93</cites><orcidid>0000-0003-0422-846X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-022-07316-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-022-07316-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35366759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, Xiao-Hui</creatorcontrib><creatorcontrib>Gu, Sheng-Ji</creatorcontrib><creatorcontrib>Tian, Wen-Jie</creatorcontrib><creatorcontrib>Song, Wen-Peng</creatorcontrib><creatorcontrib>Gu, Yang-Yang</creatorcontrib><creatorcontrib>Yuan, Yi-Ming</creatorcontrib><creatorcontrib>Li, Xue-Song</creatorcontrib><creatorcontrib>Xin, Zhong-Cheng</creatorcontrib><creatorcontrib>Kim, Sae Woong</creatorcontrib><creatorcontrib>Guan, Rui-Li</creatorcontrib><creatorcontrib>Bae, Woong Jin</creatorcontrib><title>Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated.
Methods
EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs.
Results
A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia.
Conclusions
Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell cycle</subject><subject>Clusterin</subject><subject>Enzymatic activity</subject><subject>Extracellular vesicles</subject><subject>Galectin-1</subject><subject>Histology</subject><subject>Immune system</subject><subject>Infertility</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectroscopy</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Morphology</subject><subject>Oligozoospermia</subject><subject>Original Article</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Sertoli cells</subject><subject>Spermatogenesis</subject><subject>Transmission electron microscopy</subject><subject>Ultracentrifugation</subject><subject>Western blotting</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1LHTEUhkNR6q32D3RRAm5cdOw5k8kk053IrS2IQtV1yM2HRubDJjNS_fVmvLcKLlwFcp73PQkPIV8QDhFAfE-IwFgBZVmAYFgX-IEskAtWVI2QW2QBDLCoJMcd8imlWwCoUPCPZIdxVteCNwuSlv_GqI1r26nVkd67FEzrErUuhntnqY9DR2-mTvf0wsVxaAOd4fSDmhudg2PmHvUYhj59o3dxGN3QBUN1r9uHFPKd7i3twp-zo3nqQxv66z2y7XWb3OfNuUuufi4vj38Vp-cnv4-PTgvDBB8LUYH2shLAsWRs5SVqMIASK9FwIX3JjMUVytI23FiQ3paemxp8rY0Vq4btkoN1b978d3JpVF1I8-t174YpqbKu6oZJqFlG99-gt8MU8ydmSnKJknPMVLmmTBxSis6ruxg6HR8UgpqVqLUSlZWoZyVqDn3dVE-rztmXyH8HGWBrIOVRf-3i6-53ap8A9jyXWg</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Tan, Xiao-Hui</creator><creator>Gu, 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derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling</title><author>Tan, Xiao-Hui ; Gu, Sheng-Ji ; Tian, Wen-Jie ; Song, Wen-Peng ; Gu, Yang-Yang ; Yuan, Yi-Ming ; Li, Xue-Song ; Xin, Zhong-Cheng ; Kim, Sae Woong ; Guan, Rui-Li ; Bae, Woong Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-740af847051233bf81a0c0181479578f23cd1b182d95cd08fd2f5c60f6acd7b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell cycle</topic><topic>Clusterin</topic><topic>Enzymatic activity</topic><topic>Extracellular vesicles</topic><topic>Galectin-1</topic><topic>Histology</topic><topic>Immune system</topic><topic>Infertility</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Mass spectroscopy</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Morphology</topic><topic>Oligozoospermia</topic><topic>Original Article</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Sertoli cells</topic><topic>Spermatogenesis</topic><topic>Transmission electron microscopy</topic><topic>Ultracentrifugation</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, Xiao-Hui</creatorcontrib><creatorcontrib>Gu, Sheng-Ji</creatorcontrib><creatorcontrib>Tian, Wen-Jie</creatorcontrib><creatorcontrib>Song, Wen-Peng</creatorcontrib><creatorcontrib>Gu, Yang-Yang</creatorcontrib><creatorcontrib>Yuan, Yi-Ming</creatorcontrib><creatorcontrib>Li, Xue-Song</creatorcontrib><creatorcontrib>Xin, Zhong-Cheng</creatorcontrib><creatorcontrib>Kim, Sae Woong</creatorcontrib><creatorcontrib>Guan, Rui-Li</creatorcontrib><creatorcontrib>Bae, Woong 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characterizations, proteomic analysis, and miRNA profiling</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>49</volume><issue>6</issue><spage>4673</spage><epage>4681</epage><pages>4673-4681</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated.
Methods
EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs.
Results
A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia.
Conclusions
Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>35366759</pmid><doi>10.1007/s11033-022-07316-1</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-0422-846X</orcidid></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Cell cycle Clusterin Enzymatic activity Extracellular vesicles Galectin-1 Histology Immune system Infertility Life Sciences Liquid chromatography Mass spectroscopy MicroRNAs miRNA Morphology Oligozoospermia Original Article Proteins Proteomics Sertoli cells Spermatogenesis Transmission electron microscopy Ultracentrifugation Western blotting |
| title | Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling |
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