Isolation and identification of mucin‐degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype
Aim Mucin‐degrading bacteria are known to be beneficial for gut health. We aimed to isolate human‐derived mucin‐degrading bacteria and identify potential probiotic characteristics and their effects on the bacterial community and short‐chain fatty acid (SCFA) production according to three different e...
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| Veröffentlicht in: | Journal of applied microbiology 2022-08, Vol.133 (2), p.362-374 |
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| creator | Yuan, Heng Zhou, Junyu Li, Nanxin Wu, Xuangao Huang, Shaokai Park, Sunmin |
| description | Aim
Mucin‐degrading bacteria are known to be beneficial for gut health. We aimed to isolate human‐derived mucin‐degrading bacteria and identify potential probiotic characteristics and their effects on the bacterial community and short‐chain fatty acid (SCFA) production according to three different enterotypes of the host.
Methods and Results
Bacteria with mucin decomposition ability from human faeces were isolated and identified by 16S rRNA sequencing and MALDI‐TOF. Heat resistance, acid resistance, antibiotic resistance, and antibacterial activity were analysed in the selected bacteria. Their adhesion capability to the Caco‐2 cell was determined by scanning electron microscopy. Their ability to alter the bacterial community and SCFA production of the isolated bacteria was investigated in three enterotypes. The three isolated strains were Bifidobacterium(Bif.) animalis SPM01 (CP001606.1, 99%), Bif. longum SPM02 (NR_043437.1, 99%), and Limosilactobacillus(L.) reuteri SPM03 (CP000705.1, 99%) deposited in Korean Collection for Type Culture (KCTC‐18958P). Among them, Bif. animalis exhibited the highest mucin degrading ability. They exhibited strong resistance to acidic conditions, moderate resistance to heat, and the ability to adhere tightly to Caco‐2 cells. Three isolated mucin‐degrading bacteria incubation increased Lactobacillus in the faecal bacteria from Bacteroides and Prevotella enterotypes. However, only L. reuteri elevated Lactobacillus in the faecal bacteria from the Ruminococcus enterotype. B. longum and B. animalis increased the α‐diversity in the Ruminococcus enterotype, while their incubation with other intestinal types decreased the α‐diversity. Bifidobacterium animalis and L. reuteri increased the butyric acid level in faecal bacteria from the Prevotella enterotype, and L. reuteri elevated the acetic acid level in those from the Ruminococcus enterotype. However, the overall SCFA changes were minimal.
Conclusions
The isolated mucin‐degrading bacteria act as probiotics and modulate gut microbiota and SCFA production differently according to the host′s enterotypes.
Significance and Impact of Study
Probiotics need to be personalized according to the enterotypes in clinical application. |
| doi_str_mv | 10.1111/jam.15560 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2646724841</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2646724841</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3530-9e253cc9f701cfe0f858fc56ad90d8463a3d6dfb42a160490e7e43a4511f2fb83</originalsourceid><addsrcrecordid>eNp1kU9u3CAUh1HVqEnTLnqBCqmbduEEbGDsZRT1T6pE2aRrC8NjhpGBKWBFs8sRKvUSPVdOUsZOu6hUNiD08b33-CH0hpIzWtb5Vrozyrkgz9AJbQSvarGqn89nVnGyqo_Ry5S2hNCGcPECHTe8UK2oT9CvqxRGmW3wWHqNrQafrbFquQoGu0lZ__jwQ8M6Sm39Gg9SZYhW4hDt2nqZQWMTg8ObyUmPjQQFabblDdiIdyEfpHLEuxgGG7JVGMyhhtpjqVSIszYHvAkpPz78dFbNoANcHkIMeb-DV-jIyDHB66f9FH379PHu8kt1ffv56vLiulJlKFJ1UPNGqc6sCFUGiGl5axQXUndEt0w0stFCm4HVkgrCOgIrYI1knFJTm6FtTtH7xVua_T5Byr2zScE4Sg9hSn0tWPlc1jJa0Hf_oNswRV-6K1TXdiUQcaA-LFQZKqUIpt9F62Tc95T0h_j6El8_x1fYt0_GaXCg_5J_8irA-QLc2xH2_zf1Xy9uFuVvKZqp6w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2698956061</pqid></control><display><type>article</type><title>Isolation and identification of mucin‐degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype</title><source>Wiley Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Yuan, Heng ; Zhou, Junyu ; Li, Nanxin ; Wu, Xuangao ; Huang, Shaokai ; Park, Sunmin</creator><creatorcontrib>Yuan, Heng ; Zhou, Junyu ; Li, Nanxin ; Wu, Xuangao ; Huang, Shaokai ; Park, Sunmin</creatorcontrib><description>Aim
Mucin‐degrading bacteria are known to be beneficial for gut health. We aimed to isolate human‐derived mucin‐degrading bacteria and identify potential probiotic characteristics and their effects on the bacterial community and short‐chain fatty acid (SCFA) production according to three different enterotypes of the host.
Methods and Results
Bacteria with mucin decomposition ability from human faeces were isolated and identified by 16S rRNA sequencing and MALDI‐TOF. Heat resistance, acid resistance, antibiotic resistance, and antibacterial activity were analysed in the selected bacteria. Their adhesion capability to the Caco‐2 cell was determined by scanning electron microscopy. Their ability to alter the bacterial community and SCFA production of the isolated bacteria was investigated in three enterotypes. The three isolated strains were Bifidobacterium(Bif.) animalis SPM01 (CP001606.1, 99%), Bif. longum SPM02 (NR_043437.1, 99%), and Limosilactobacillus(L.) reuteri SPM03 (CP000705.1, 99%) deposited in Korean Collection for Type Culture (KCTC‐18958P). Among them, Bif. animalis exhibited the highest mucin degrading ability. They exhibited strong resistance to acidic conditions, moderate resistance to heat, and the ability to adhere tightly to Caco‐2 cells. Three isolated mucin‐degrading bacteria incubation increased Lactobacillus in the faecal bacteria from Bacteroides and Prevotella enterotypes. However, only L. reuteri elevated Lactobacillus in the faecal bacteria from the Ruminococcus enterotype. B. longum and B. animalis increased the α‐diversity in the Ruminococcus enterotype, while their incubation with other intestinal types decreased the α‐diversity. Bifidobacterium animalis and L. reuteri increased the butyric acid level in faecal bacteria from the Prevotella enterotype, and L. reuteri elevated the acetic acid level in those from the Ruminococcus enterotype. However, the overall SCFA changes were minimal.
Conclusions
The isolated mucin‐degrading bacteria act as probiotics and modulate gut microbiota and SCFA production differently according to the host′s enterotypes.
Significance and Impact of Study
Probiotics need to be personalized according to the enterotypes in clinical application.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15560</identifier><identifier>PMID: 35365862</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Acetic acid ; Acid resistance ; Antibacterial activity ; Antibiotic resistance ; Antibiotics ; Bacteria ; Bifidobacterium animalis ; Bifidobacterium longum ; Biodegradation ; Butyric acid ; Cell culture ; Degradation ; Digestive system ; Fatty acids ; Feces ; Gastrointestinal tract ; gut microbiota ; Heat resistance ; Human performance ; in vitro ; intestinal adhesion ; Intestinal microflora ; Lactobacillus ; Limosilactobacillus reuteri ; Microbiomes ; Microbiota ; Mucin ; Prevotella ; Probiotics ; rRNA 16S ; Ruminococcus ; Scanning electron microscopy ; stress resistance ; Thermal resistance</subject><ispartof>Journal of applied microbiology, 2022-08, Vol.133 (2), p.362-374</ispartof><rights>2022 Society for Applied Microbiology.</rights><rights>Copyright © 2022 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-9e253cc9f701cfe0f858fc56ad90d8463a3d6dfb42a160490e7e43a4511f2fb83</citedby><cites>FETCH-LOGICAL-c3530-9e253cc9f701cfe0f858fc56ad90d8463a3d6dfb42a160490e7e43a4511f2fb83</cites><orcidid>0000-0002-6092-8340</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.15560$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.15560$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35365862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Heng</creatorcontrib><creatorcontrib>Zhou, Junyu</creatorcontrib><creatorcontrib>Li, Nanxin</creatorcontrib><creatorcontrib>Wu, Xuangao</creatorcontrib><creatorcontrib>Huang, Shaokai</creatorcontrib><creatorcontrib>Park, Sunmin</creatorcontrib><title>Isolation and identification of mucin‐degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
Mucin‐degrading bacteria are known to be beneficial for gut health. We aimed to isolate human‐derived mucin‐degrading bacteria and identify potential probiotic characteristics and their effects on the bacterial community and short‐chain fatty acid (SCFA) production according to three different enterotypes of the host.
Methods and Results
Bacteria with mucin decomposition ability from human faeces were isolated and identified by 16S rRNA sequencing and MALDI‐TOF. Heat resistance, acid resistance, antibiotic resistance, and antibacterial activity were analysed in the selected bacteria. Their adhesion capability to the Caco‐2 cell was determined by scanning electron microscopy. Their ability to alter the bacterial community and SCFA production of the isolated bacteria was investigated in three enterotypes. The three isolated strains were Bifidobacterium(Bif.) animalis SPM01 (CP001606.1, 99%), Bif. longum SPM02 (NR_043437.1, 99%), and Limosilactobacillus(L.) reuteri SPM03 (CP000705.1, 99%) deposited in Korean Collection for Type Culture (KCTC‐18958P). Among them, Bif. animalis exhibited the highest mucin degrading ability. They exhibited strong resistance to acidic conditions, moderate resistance to heat, and the ability to adhere tightly to Caco‐2 cells. Three isolated mucin‐degrading bacteria incubation increased Lactobacillus in the faecal bacteria from Bacteroides and Prevotella enterotypes. However, only L. reuteri elevated Lactobacillus in the faecal bacteria from the Ruminococcus enterotype. B. longum and B. animalis increased the α‐diversity in the Ruminococcus enterotype, while their incubation with other intestinal types decreased the α‐diversity. Bifidobacterium animalis and L. reuteri increased the butyric acid level in faecal bacteria from the Prevotella enterotype, and L. reuteri elevated the acetic acid level in those from the Ruminococcus enterotype. However, the overall SCFA changes were minimal.
Conclusions
The isolated mucin‐degrading bacteria act as probiotics and modulate gut microbiota and SCFA production differently according to the host′s enterotypes.
Significance and Impact of Study
Probiotics need to be personalized according to the enterotypes in clinical application.</description><subject>Acetic acid</subject><subject>Acid resistance</subject><subject>Antibacterial activity</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bifidobacterium animalis</subject><subject>Bifidobacterium longum</subject><subject>Biodegradation</subject><subject>Butyric acid</subject><subject>Cell culture</subject><subject>Degradation</subject><subject>Digestive system</subject><subject>Fatty acids</subject><subject>Feces</subject><subject>Gastrointestinal tract</subject><subject>gut microbiota</subject><subject>Heat resistance</subject><subject>Human performance</subject><subject>in vitro</subject><subject>intestinal adhesion</subject><subject>Intestinal microflora</subject><subject>Lactobacillus</subject><subject>Limosilactobacillus reuteri</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Mucin</subject><subject>Prevotella</subject><subject>Probiotics</subject><subject>rRNA 16S</subject><subject>Ruminococcus</subject><subject>Scanning electron microscopy</subject><subject>stress resistance</subject><subject>Thermal resistance</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kU9u3CAUh1HVqEnTLnqBCqmbduEEbGDsZRT1T6pE2aRrC8NjhpGBKWBFs8sRKvUSPVdOUsZOu6hUNiD08b33-CH0hpIzWtb5Vrozyrkgz9AJbQSvarGqn89nVnGyqo_Ry5S2hNCGcPECHTe8UK2oT9CvqxRGmW3wWHqNrQafrbFquQoGu0lZ__jwQ8M6Sm39Gg9SZYhW4hDt2nqZQWMTg8ObyUmPjQQFabblDdiIdyEfpHLEuxgGG7JVGMyhhtpjqVSIszYHvAkpPz78dFbNoANcHkIMeb-DV-jIyDHB66f9FH379PHu8kt1ffv56vLiulJlKFJ1UPNGqc6sCFUGiGl5axQXUndEt0w0stFCm4HVkgrCOgIrYI1knFJTm6FtTtH7xVua_T5Byr2zScE4Sg9hSn0tWPlc1jJa0Hf_oNswRV-6K1TXdiUQcaA-LFQZKqUIpt9F62Tc95T0h_j6El8_x1fYt0_GaXCg_5J_8irA-QLc2xH2_zf1Xy9uFuVvKZqp6w</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Yuan, Heng</creator><creator>Zhou, Junyu</creator><creator>Li, Nanxin</creator><creator>Wu, Xuangao</creator><creator>Huang, Shaokai</creator><creator>Park, Sunmin</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6092-8340</orcidid></search><sort><creationdate>202208</creationdate><title>Isolation and identification of mucin‐degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype</title><author>Yuan, Heng ; Zhou, Junyu ; Li, Nanxin ; Wu, Xuangao ; Huang, Shaokai ; Park, Sunmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-9e253cc9f701cfe0f858fc56ad90d8463a3d6dfb42a160490e7e43a4511f2fb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acetic acid</topic><topic>Acid resistance</topic><topic>Antibacterial activity</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bifidobacterium animalis</topic><topic>Bifidobacterium longum</topic><topic>Biodegradation</topic><topic>Butyric acid</topic><topic>Cell culture</topic><topic>Degradation</topic><topic>Digestive system</topic><topic>Fatty acids</topic><topic>Feces</topic><topic>Gastrointestinal tract</topic><topic>gut microbiota</topic><topic>Heat resistance</topic><topic>Human performance</topic><topic>in vitro</topic><topic>intestinal adhesion</topic><topic>Intestinal microflora</topic><topic>Lactobacillus</topic><topic>Limosilactobacillus reuteri</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>Mucin</topic><topic>Prevotella</topic><topic>Probiotics</topic><topic>rRNA 16S</topic><topic>Ruminococcus</topic><topic>Scanning electron microscopy</topic><topic>stress resistance</topic><topic>Thermal resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Heng</creatorcontrib><creatorcontrib>Zhou, Junyu</creatorcontrib><creatorcontrib>Li, Nanxin</creatorcontrib><creatorcontrib>Wu, Xuangao</creatorcontrib><creatorcontrib>Huang, Shaokai</creatorcontrib><creatorcontrib>Park, Sunmin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Heng</au><au>Zhou, Junyu</au><au>Li, Nanxin</au><au>Wu, Xuangao</au><au>Huang, Shaokai</au><au>Park, Sunmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and identification of mucin‐degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2022-08</date><risdate>2022</risdate><volume>133</volume><issue>2</issue><spage>362</spage><epage>374</epage><pages>362-374</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
Mucin‐degrading bacteria are known to be beneficial for gut health. We aimed to isolate human‐derived mucin‐degrading bacteria and identify potential probiotic characteristics and their effects on the bacterial community and short‐chain fatty acid (SCFA) production according to three different enterotypes of the host.
Methods and Results
Bacteria with mucin decomposition ability from human faeces were isolated and identified by 16S rRNA sequencing and MALDI‐TOF. Heat resistance, acid resistance, antibiotic resistance, and antibacterial activity were analysed in the selected bacteria. Their adhesion capability to the Caco‐2 cell was determined by scanning electron microscopy. Their ability to alter the bacterial community and SCFA production of the isolated bacteria was investigated in three enterotypes. The three isolated strains were Bifidobacterium(Bif.) animalis SPM01 (CP001606.1, 99%), Bif. longum SPM02 (NR_043437.1, 99%), and Limosilactobacillus(L.) reuteri SPM03 (CP000705.1, 99%) deposited in Korean Collection for Type Culture (KCTC‐18958P). Among them, Bif. animalis exhibited the highest mucin degrading ability. They exhibited strong resistance to acidic conditions, moderate resistance to heat, and the ability to adhere tightly to Caco‐2 cells. Three isolated mucin‐degrading bacteria incubation increased Lactobacillus in the faecal bacteria from Bacteroides and Prevotella enterotypes. However, only L. reuteri elevated Lactobacillus in the faecal bacteria from the Ruminococcus enterotype. B. longum and B. animalis increased the α‐diversity in the Ruminococcus enterotype, while their incubation with other intestinal types decreased the α‐diversity. Bifidobacterium animalis and L. reuteri increased the butyric acid level in faecal bacteria from the Prevotella enterotype, and L. reuteri elevated the acetic acid level in those from the Ruminococcus enterotype. However, the overall SCFA changes were minimal.
Conclusions
The isolated mucin‐degrading bacteria act as probiotics and modulate gut microbiota and SCFA production differently according to the host′s enterotypes.
Significance and Impact of Study
Probiotics need to be personalized according to the enterotypes in clinical application.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>35365862</pmid><doi>10.1111/jam.15560</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-6092-8340</orcidid></addata></record> |
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| source | Wiley Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Acetic acid Acid resistance Antibacterial activity Antibiotic resistance Antibiotics Bacteria Bifidobacterium animalis Bifidobacterium longum Biodegradation Butyric acid Cell culture Degradation Digestive system Fatty acids Feces Gastrointestinal tract gut microbiota Heat resistance Human performance in vitro intestinal adhesion Intestinal microflora Lactobacillus Limosilactobacillus reuteri Microbiomes Microbiota Mucin Prevotella Probiotics rRNA 16S Ruminococcus Scanning electron microscopy stress resistance Thermal resistance |
| title | Isolation and identification of mucin‐degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype |
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