Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme
Alchemilla viridiflora Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycoside...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2022-07, Vol.477 (7), p.1893-1903 |
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| container_issue | 7 |
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| container_title | Molecular and cellular biochemistry |
| container_volume | 477 |
| creator | Radovic, Jelena Surucic, Relja Niketic, Marjan Kundakovic-Vasovic, Tatjana |
| description | Alchemilla viridiflora
Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose-dependent ACE inhibitory activities of
A. viridiflora
and miquelianin were observed with an IC
50
of 2.51 ± 0.00 µg/mL of
A. viridiflora
extract compared to the IC
50
of 5.4139 ± 0.00 µM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay. |
| doi_str_mv | 10.1007/s11010-022-04410-7 |
| format | Article |
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Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose-dependent ACE inhibitory activities of
A. viridiflora
and miquelianin were observed with an IC
50
of 2.51 ± 0.00 µg/mL of
A. viridiflora
extract compared to the IC
50
of 5.4139 ± 0.00 µM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-022-04410-7</identifier><identifier>PMID: 35348979</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Alchemilla ; Analysis ; Angiotensin ; Angiotensin I ; Biochemistry ; Bioflavonoids ; Biological products ; Biomedical and Life Sciences ; Cardiology ; Computer applications ; Conversion ; Ellagic acid ; Enzymes ; Flavone glycosides ; Flavones ; Flavonoids ; Glycosides ; Hypertension ; Life Sciences ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Medical Biochemistry ; Methanol ; Oncology ; Pentose ; Peptidyl-dipeptidase A ; Plant extracts</subject><ispartof>Molecular and cellular biochemistry, 2022-07, Vol.477 (7), p.1893-1903</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>COPYRIGHT 2022 Springer</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-e7d5af00ba96bc7255561002a618904b7d2a0cbcdbee33e23171d13e787877bb3</citedby><cites>FETCH-LOGICAL-c405t-e7d5af00ba96bc7255561002a618904b7d2a0cbcdbee33e23171d13e787877bb3</cites><orcidid>0000-0001-5777-8612</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-022-04410-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-022-04410-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35348979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radovic, Jelena</creatorcontrib><creatorcontrib>Surucic, Relja</creatorcontrib><creatorcontrib>Niketic, Marjan</creatorcontrib><creatorcontrib>Kundakovic-Vasovic, Tatjana</creatorcontrib><title>Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Alchemilla viridiflora
Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose-dependent ACE inhibitory activities of
A. viridiflora
and miquelianin were observed with an IC
50
of 2.51 ± 0.00 µg/mL of
A. viridiflora
extract compared to the IC
50
of 5.4139 ± 0.00 µM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.</description><subject>Alchemilla</subject><subject>Analysis</subject><subject>Angiotensin</subject><subject>Angiotensin I</subject><subject>Biochemistry</subject><subject>Bioflavonoids</subject><subject>Biological products</subject><subject>Biomedical and Life Sciences</subject><subject>Cardiology</subject><subject>Computer applications</subject><subject>Conversion</subject><subject>Ellagic acid</subject><subject>Enzymes</subject><subject>Flavone glycosides</subject><subject>Flavones</subject><subject>Flavonoids</subject><subject>Glycosides</subject><subject>Hypertension</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical Biochemistry</subject><subject>Methanol</subject><subject>Oncology</subject><subject>Pentose</subject><subject>Peptidyl-dipeptidase A</subject><subject>Plant 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biochemistry</jtitle><stitle>Mol Cell Biochem</stitle><addtitle>Mol Cell Biochem</addtitle><date>2022-07-01</date><risdate>2022</risdate><volume>477</volume><issue>7</issue><spage>1893</spage><epage>1903</epage><pages>1893-1903</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Alchemilla viridiflora
Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose-dependent ACE inhibitory activities of
A. viridiflora
and miquelianin were observed with an IC
50
of 2.51 ± 0.00 µg/mL of
A. viridiflora
extract compared to the IC
50
of 5.4139 ± 0.00 µM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35348979</pmid><doi>10.1007/s11010-022-04410-7</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-5777-8612</orcidid></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 2022-07, Vol.477 (7), p.1893-1903 |
| issn | 0300-8177 1573-4919 |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Alchemilla Analysis Angiotensin Angiotensin I Biochemistry Bioflavonoids Biological products Biomedical and Life Sciences Cardiology Computer applications Conversion Ellagic acid Enzymes Flavone glycosides Flavones Flavonoids Glycosides Hypertension Life Sciences Liquid chromatography Mass spectrometry Mass spectroscopy Medical Biochemistry Methanol Oncology Pentose Peptidyl-dipeptidase A Plant extracts |
| title | Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme |
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