A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications
Ductal carcinoma in situ (DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform inf...
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| Veröffentlicht in: | Analyst (London) 2022-04, Vol.147 (8), p.1641-1654 |
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| creator | Gosling, Sarah Calabrese, Doriana Nallala, Jayakrupakar Greenwood, Charlene Pinder, Sarah King, Lorraine Marks, Jeffrey Pinto, Donna Lynch, Thomas Lyburn, Iain D Hwang, E. Shelley Grand Challenge PRECISION Consortium Rogers, Keith Stone, Nicholas |
| description | Ductal carcinoma
in situ
(DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform infrared spectroscopy were used to determine the physicochemical and crystallographic properties of type II breast calcifications located in formalin fixed paraffin embedded DCIS breast tissue samples. Multiple calcium phosphate phases were identified across the calcifications, distributed in different patterns. Hydroxyapatite was the dominant mineral, with magnesium whitlockite found at the calcification edge. Amorphous calcium phosphate and octacalcium phosphate were also identified close to the calcification edge at the apparent mineral/matrix barrier. Crystallographic features of hydroxyapatite also varied across the calcifications, with higher crystallinity centrally, and highest carbonate substitution at the calcification edge. Protein was also differentially distributed across the calcification and the surrounding soft tissue, with collagen and β-pleated protein features present to differing extents. Combination of analytical techniques in this study was essential to understand the heterogeneity of breast calcifications and how this may link crystallographic and physicochemical properties of calcifications to the surrounding tissue microenvironment.
Combined crystallographic and spectroscopic methods were used to investigate the heterogeneity of breast calcifications found associated with ductal carcinoma
in situ
, revealing distinctive patterns in protein distribution and mineral composition. |
| doi_str_mv | 10.1039/d1an01548f |
| format | Article |
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in situ
(DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform infrared spectroscopy were used to determine the physicochemical and crystallographic properties of type II breast calcifications located in formalin fixed paraffin embedded DCIS breast tissue samples. Multiple calcium phosphate phases were identified across the calcifications, distributed in different patterns. Hydroxyapatite was the dominant mineral, with magnesium whitlockite found at the calcification edge. Amorphous calcium phosphate and octacalcium phosphate were also identified close to the calcification edge at the apparent mineral/matrix barrier. Crystallographic features of hydroxyapatite also varied across the calcifications, with higher crystallinity centrally, and highest carbonate substitution at the calcification edge. Protein was also differentially distributed across the calcification and the surrounding soft tissue, with collagen and β-pleated protein features present to differing extents. Combination of analytical techniques in this study was essential to understand the heterogeneity of breast calcifications and how this may link crystallographic and physicochemical properties of calcifications to the surrounding tissue microenvironment.
Combined crystallographic and spectroscopic methods were used to investigate the heterogeneity of breast calcifications found associated with ductal carcinoma
in situ
, revealing distinctive patterns in protein distribution and mineral composition.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d1an01548f</identifier><identifier>PMID: 35311860</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Breast ; Breast Neoplasms ; Calcification ; Calcinosis - pathology ; Calcium phosphates ; Carcinoma, Intraductal, Noninfiltrating - pathology ; Chemical properties ; Chemistry ; Crystallography ; Durapatite ; Female ; Fourier transforms ; Heterogeneity ; Humans ; Hydroxyapatite ; Magnesium ; Paraffins ; Proteins ; Soft tissues ; Spectroscopy, Fourier Transform Infrared ; Tumor Microenvironment ; X-Ray Diffraction</subject><ispartof>Analyst (London), 2022-04, Vol.147 (8), p.1641-1654</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><rights>This journal is © The Royal Society of Chemistry 2022 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-2f6932c17847cd94aa366026c8f7c140192a4bf404f7773712bda42e40355233</citedby><cites>FETCH-LOGICAL-c428t-2f6932c17847cd94aa366026c8f7c140192a4bf404f7773712bda42e40355233</cites><orcidid>0000-0002-7034-6547 ; 0000-0001-5603-3731</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,2818,2819,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35311860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gosling, Sarah</creatorcontrib><creatorcontrib>Calabrese, Doriana</creatorcontrib><creatorcontrib>Nallala, Jayakrupakar</creatorcontrib><creatorcontrib>Greenwood, Charlene</creatorcontrib><creatorcontrib>Pinder, Sarah</creatorcontrib><creatorcontrib>King, Lorraine</creatorcontrib><creatorcontrib>Marks, Jeffrey</creatorcontrib><creatorcontrib>Pinto, Donna</creatorcontrib><creatorcontrib>Lynch, Thomas</creatorcontrib><creatorcontrib>Lyburn, Iain D</creatorcontrib><creatorcontrib>Hwang, E. Shelley</creatorcontrib><creatorcontrib>Grand Challenge PRECISION Consortium</creatorcontrib><creatorcontrib>Rogers, Keith</creatorcontrib><creatorcontrib>Stone, Nicholas</creatorcontrib><title>A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Ductal carcinoma
in situ
(DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform infrared spectroscopy were used to determine the physicochemical and crystallographic properties of type II breast calcifications located in formalin fixed paraffin embedded DCIS breast tissue samples. Multiple calcium phosphate phases were identified across the calcifications, distributed in different patterns. Hydroxyapatite was the dominant mineral, with magnesium whitlockite found at the calcification edge. Amorphous calcium phosphate and octacalcium phosphate were also identified close to the calcification edge at the apparent mineral/matrix barrier. Crystallographic features of hydroxyapatite also varied across the calcifications, with higher crystallinity centrally, and highest carbonate substitution at the calcification edge. Protein was also differentially distributed across the calcification and the surrounding soft tissue, with collagen and β-pleated protein features present to differing extents. Combination of analytical techniques in this study was essential to understand the heterogeneity of breast calcifications and how this may link crystallographic and physicochemical properties of calcifications to the surrounding tissue microenvironment.
Combined crystallographic and spectroscopic methods were used to investigate the heterogeneity of breast calcifications found associated with ductal carcinoma
in situ
, revealing distinctive patterns in protein distribution and mineral composition.</description><subject>Breast</subject><subject>Breast Neoplasms</subject><subject>Calcification</subject><subject>Calcinosis - pathology</subject><subject>Calcium phosphates</subject><subject>Carcinoma, Intraductal, Noninfiltrating - pathology</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Crystallography</subject><subject>Durapatite</subject><subject>Female</subject><subject>Fourier transforms</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Magnesium</subject><subject>Paraffins</subject><subject>Proteins</subject><subject>Soft tissues</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Tumor Microenvironment</subject><subject>X-Ray Diffraction</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks1r2zAYxkVZabJ2l91XDLuMgVu9kmzLl0JIlrUQ2sN6F4osNQq25Un2WP77yUmbtT29iOfH8348Qugz4CvAtLyuQLYYMsbNCZoCzVmaZYR_QFOMMU1JnrEJ-hjCNj4BZ_gMTWhGAXiOp8jOkmaoe5s2rpJ1ov92tfOyt65NnEk2utfePelWuyEk3WYXrHKp2ujGqkh33nXa91aHEV7M734la69l6JOoexcRZU0kR7twgU6NrIP-9FzP0ePyx-P8Nl09_Lybz1apYoT3KTF5SYmCgrNCVSWTkuY5JrniplDAMJREsrVhmJmiKGgBZF1JRjTDNG5N6Tm6Odh2w7rRldJt72UtOm8b6XfCSSveKq3diCf3R_CyjHYsGnx7NvDu96BDLxoblK5rub-CIDmDDDjmY6-v79CtG3wbtxspXhYZYBKp7wcqniQEr81xGMBiDFAsYHa_D3AZ4cvX4x_Rl8Qi8OUA-KCO6v8fQP8Bhp6gtQ</recordid><startdate>20220411</startdate><enddate>20220411</enddate><creator>Gosling, Sarah</creator><creator>Calabrese, Doriana</creator><creator>Nallala, Jayakrupakar</creator><creator>Greenwood, Charlene</creator><creator>Pinder, Sarah</creator><creator>King, Lorraine</creator><creator>Marks, Jeffrey</creator><creator>Pinto, Donna</creator><creator>Lynch, Thomas</creator><creator>Lyburn, Iain D</creator><creator>Hwang, E. Shelley</creator><creator>Grand Challenge PRECISION Consortium</creator><creator>Rogers, Keith</creator><creator>Stone, Nicholas</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7034-6547</orcidid><orcidid>https://orcid.org/0000-0001-5603-3731</orcidid></search><sort><creationdate>20220411</creationdate><title>A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications</title><author>Gosling, Sarah ; Calabrese, Doriana ; Nallala, Jayakrupakar ; Greenwood, Charlene ; Pinder, Sarah ; King, Lorraine ; Marks, Jeffrey ; Pinto, Donna ; Lynch, Thomas ; Lyburn, Iain D ; Hwang, E. Shelley ; Grand Challenge PRECISION Consortium ; Rogers, Keith ; Stone, Nicholas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-2f6932c17847cd94aa366026c8f7c140192a4bf404f7773712bda42e40355233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Breast</topic><topic>Breast Neoplasms</topic><topic>Calcification</topic><topic>Calcinosis - pathology</topic><topic>Calcium phosphates</topic><topic>Carcinoma, Intraductal, Noninfiltrating - pathology</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Crystallography</topic><topic>Durapatite</topic><topic>Female</topic><topic>Fourier transforms</topic><topic>Heterogeneity</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Magnesium</topic><topic>Paraffins</topic><topic>Proteins</topic><topic>Soft tissues</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Tumor Microenvironment</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gosling, Sarah</creatorcontrib><creatorcontrib>Calabrese, Doriana</creatorcontrib><creatorcontrib>Nallala, Jayakrupakar</creatorcontrib><creatorcontrib>Greenwood, Charlene</creatorcontrib><creatorcontrib>Pinder, Sarah</creatorcontrib><creatorcontrib>King, Lorraine</creatorcontrib><creatorcontrib>Marks, Jeffrey</creatorcontrib><creatorcontrib>Pinto, Donna</creatorcontrib><creatorcontrib>Lynch, Thomas</creatorcontrib><creatorcontrib>Lyburn, Iain D</creatorcontrib><creatorcontrib>Hwang, E. Shelley</creatorcontrib><creatorcontrib>Grand Challenge PRECISION Consortium</creatorcontrib><creatorcontrib>Rogers, Keith</creatorcontrib><creatorcontrib>Stone, Nicholas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gosling, Sarah</au><au>Calabrese, Doriana</au><au>Nallala, Jayakrupakar</au><au>Greenwood, Charlene</au><au>Pinder, Sarah</au><au>King, Lorraine</au><au>Marks, Jeffrey</au><au>Pinto, Donna</au><au>Lynch, Thomas</au><au>Lyburn, Iain D</au><au>Hwang, E. Shelley</au><au>Grand Challenge PRECISION Consortium</au><au>Rogers, Keith</au><au>Stone, Nicholas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2022-04-11</date><risdate>2022</risdate><volume>147</volume><issue>8</issue><spage>1641</spage><epage>1654</epage><pages>1641-1654</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Ductal carcinoma
in situ
(DCIS) is frequently associated with breast calcification. This study combines multiple analytical techniques to investigate the heterogeneity of these calcifications at the micrometre scale. X-ray diffraction, scanning electron microscopy and Raman and Fourier-transform infrared spectroscopy were used to determine the physicochemical and crystallographic properties of type II breast calcifications located in formalin fixed paraffin embedded DCIS breast tissue samples. Multiple calcium phosphate phases were identified across the calcifications, distributed in different patterns. Hydroxyapatite was the dominant mineral, with magnesium whitlockite found at the calcification edge. Amorphous calcium phosphate and octacalcium phosphate were also identified close to the calcification edge at the apparent mineral/matrix barrier. Crystallographic features of hydroxyapatite also varied across the calcifications, with higher crystallinity centrally, and highest carbonate substitution at the calcification edge. Protein was also differentially distributed across the calcification and the surrounding soft tissue, with collagen and β-pleated protein features present to differing extents. Combination of analytical techniques in this study was essential to understand the heterogeneity of breast calcifications and how this may link crystallographic and physicochemical properties of calcifications to the surrounding tissue microenvironment.
Combined crystallographic and spectroscopic methods were used to investigate the heterogeneity of breast calcifications found associated with ductal carcinoma
in situ
, revealing distinctive patterns in protein distribution and mineral composition.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35311860</pmid><doi>10.1039/d1an01548f</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-7034-6547</orcidid><orcidid>https://orcid.org/0000-0001-5603-3731</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2022-04, Vol.147 (8), p.1641-1654 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2641518083 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Breast Breast Neoplasms Calcification Calcinosis - pathology Calcium phosphates Carcinoma, Intraductal, Noninfiltrating - pathology Chemical properties Chemistry Crystallography Durapatite Female Fourier transforms Heterogeneity Humans Hydroxyapatite Magnesium Paraffins Proteins Soft tissues Spectroscopy, Fourier Transform Infrared Tumor Microenvironment X-Ray Diffraction |
| title | A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications |
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