Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae

This study aimed to develop an effective method for the expression and purification of the Dermatophagoides farinae serpin protein and to establish an experimental foundation for elucidating its role in the temperature stress response. The total RNA of D. farinae was extracted, and specific primers...

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Veröffentlicht in:	Protein expression and purification 2022-08, Vol.195-196, p.106080-106080, Article 106080
Hauptverfasser:	Zhang, Wanyu, Zhao, Yae, Hu, Li, Guan, Chenglin, Xun, Meng, Wu, Feng, Lei, Yanjun
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cloning, Molecular Dermatophagoides farinae Dermatophagoides farinae - genetics Ni-NTA purification Prokaryotic expression Recombinant Proteins - genetics Serpin Serpins - genetics
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