CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver

Genetically engineered mouse models (GEMMs) transformed the study of organismal disease phenotypes but are limited by their lengthy generation in embryonic stem cells. Here, we describe methods for rapid and scalable genome engineering in somatic cells of the liver and pancreas through delivery of C...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature protocols 2022-04, Vol.17 (4), p.1142-1188
Hauptverfasser:	Kaltenbacher, Thorsten, Löprich, Jessica, Maresch, Roman, Weber, Julia, Müller, Sebastian, Oellinger, Rupert, Groß, Nina, Griger, Joscha, de Andrade Krätzig, Niklas, Avramopoulos, Petros, Ramanujam, Deepak, Brummer, Sabine, Widholz, Sebastian A., Bärthel, Stefanie, Falcomatà, Chiara, Pfaus, Anja, Alnatsha, Ahmed, Mayerle, Julia, Schmidt-Supprian, Marc, Reichert, Maximilian, Schneider, Günter, Ehmer, Ursula, Braun, Christian J., Saur, Dieter, Engelhardt, Stefan, Rad, Roland
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1511 631/1647/767/70 631/208/4041/3196 631/67/1504/1610 631/67/1504/1713 Analytical Chemistry Animal models Animals Biological Techniques Biomedical and Life Sciences Cancer Chromosomes Clustered Regularly Interspaced Short Palindromic Repeats - genetics Computational Biology/Bioinformatics CRISPR CRISPR-Cas Systems - genetics Data analysis Design of experiments Embryo cells Experimental design Gene Editing - methods Genetic engineering Genetic modification Genome editing Genomes Hepatocytes Life Sciences Liver Mice Mice, Knockout Microarrays Modelling Multiplexing Neoplasms - genetics Organic Chemistry Pancreas Phenotypes Protocol Somatic cells Stem cell transplantation Stem cells
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!