Phosphorylation of DUF1639 protein by osmotic stress/ABA-activated protein kinase 10 regulates abscisic acid-induced antioxidant defense in rice
In rice (Oryza Sativa), Osmotic Stress/ABA-activated Protein Kinase 10 (SAPK10) has been shown to be induced by hyperosmotic stress and abscisic acid (ABA). However, the molecular function of SAPK10 and its downstream targets in ABA-induced antioxidant defense is poorly understood. Here, we identifi...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2022-05, Vol.604, p.30-36 |
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| creator | Qin, Caihua Fang, Qianqian Fan, Xing Chen, Min Jiang, Mingyi |
| description | In rice (Oryza Sativa), Osmotic Stress/ABA-activated Protein Kinase 10 (SAPK10) has been shown to be induced by hyperosmotic stress and abscisic acid (ABA). However, the molecular function of SAPK10 and its downstream targets in ABA-induced antioxidant defense is poorly understood. Here, we identified an unknown function DUF1639 family protein, OsDUF1639.1, which interacts with SAPK10 in vitro and in vivo. OsDUF1639.1 positively regulates ABA responses in seed germination and tolerance to drought stress. We found that SAPK10 directly phosphorylates OsDUF1639.1 at Thr-80 in vitro. The transient expression analysis in combination with mutant analysis in rice protoplasts showed that Thr-80 is essential for ABA-induced stimulation of antioxidant defense by SAPK10. These results suggest that SAPK10 functions upstream of OsDUF1639.1 to regulate the activities of antioxidant enzymes, and Thr-80 phosphorylation of OsDUF1639.1 has a crucial role in ABA-induced antioxidant defense.
•OsDUF1639.1 positively regulates the ABA responses and tolerance to drought stress in rice.•OsDUF1639.1 interacting with SAPK10 affects ABA induced antioxidant defense.•Thr-80 phosphorylation of OsDUF1639.1 by SAPK10 is required for the effect on ABA-induced antioxidant defense. |
| doi_str_mv | 10.1016/j.bbrc.2022.03.003 |
| format | Article |
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•OsDUF1639.1 positively regulates the ABA responses and tolerance to drought stress in rice.•OsDUF1639.1 interacting with SAPK10 affects ABA induced antioxidant defense.•Thr-80 phosphorylation of OsDUF1639.1 by SAPK10 is required for the effect on ABA-induced antioxidant defense.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2022.03.003</identifier><identifier>PMID: 35279443</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Abscisic Acid - metabolism ; Antioxidant defense ; Antioxidants - metabolism ; Drought stress ; Droughts ; Gene Expression Regulation, Plant ; Oryza - metabolism ; OsDUF1639.1 ; Osmotic Pressure ; Phosphorylation ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Kinases - metabolism ; SAPK10 ; Stress, Physiological</subject><ispartof>Biochemical and biophysical research communications, 2022-05, Vol.604, p.30-36</ispartof><rights>2022 Elsevier Inc.</rights><rights>Copyright © 2022 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-2aae2a49fb835e40dce8e3a657460d17d7207e530cb9d3fd7552edbfb00d65893</citedby><cites>FETCH-LOGICAL-c356t-2aae2a49fb835e40dce8e3a657460d17d7207e530cb9d3fd7552edbfb00d65893</cites><orcidid>0000-0001-6782-0394</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2022.03.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35279443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qin, Caihua</creatorcontrib><creatorcontrib>Fang, Qianqian</creatorcontrib><creatorcontrib>Fan, Xing</creatorcontrib><creatorcontrib>Chen, Min</creatorcontrib><creatorcontrib>Jiang, Mingyi</creatorcontrib><title>Phosphorylation of DUF1639 protein by osmotic stress/ABA-activated protein kinase 10 regulates abscisic acid-induced antioxidant defense in rice</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In rice (Oryza Sativa), Osmotic Stress/ABA-activated Protein Kinase 10 (SAPK10) has been shown to be induced by hyperosmotic stress and abscisic acid (ABA). However, the molecular function of SAPK10 and its downstream targets in ABA-induced antioxidant defense is poorly understood. Here, we identified an unknown function DUF1639 family protein, OsDUF1639.1, which interacts with SAPK10 in vitro and in vivo. OsDUF1639.1 positively regulates ABA responses in seed germination and tolerance to drought stress. We found that SAPK10 directly phosphorylates OsDUF1639.1 at Thr-80 in vitro. The transient expression analysis in combination with mutant analysis in rice protoplasts showed that Thr-80 is essential for ABA-induced stimulation of antioxidant defense by SAPK10. These results suggest that SAPK10 functions upstream of OsDUF1639.1 to regulate the activities of antioxidant enzymes, and Thr-80 phosphorylation of OsDUF1639.1 has a crucial role in ABA-induced antioxidant defense.
•OsDUF1639.1 positively regulates the ABA responses and tolerance to drought stress in rice.•OsDUF1639.1 interacting with SAPK10 affects ABA induced antioxidant defense.•Thr-80 phosphorylation of OsDUF1639.1 by SAPK10 is required for the effect on ABA-induced antioxidant defense.</description><subject>Abscisic Acid - metabolism</subject><subject>Antioxidant defense</subject><subject>Antioxidants - metabolism</subject><subject>Drought stress</subject><subject>Droughts</subject><subject>Gene Expression Regulation, Plant</subject><subject>Oryza - metabolism</subject><subject>OsDUF1639.1</subject><subject>Osmotic Pressure</subject><subject>Phosphorylation</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Kinases - metabolism</subject><subject>SAPK10</subject><subject>Stress, Physiological</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1uFDEQhC0EIkvgBTggH7nMpG3PeMYSlyUhgBQJDkTiZvmnh3jZHS-2J2LfgkfGqw05cupLfdXdVYS8ZtAyYPJi01qbXMuB8xZECyCekBUDBQ1n0D0lKwCQDVfs-xl5kfMGgLFOqufkTPR8UF0nVuTP17uY93cxHbamhDjTONGr22smhaL7FAuGmdoDjXkXS3A0l4Q5X6zfrxvjSrg3Bf2j7meYTUbKgCb8sVQ_zNTY7EKupHHBN2H2i6uEmeuu38HXST1OOFesGqTg8CV5NpltxlcP85zcXn_4dvmpufny8fPl-qZxopel4cYgN52a7Ch67MA7HFEY2Q-dBM8GP3AYsBfgrPJi8kPfc_R2sgBe9qMS5-Ttybde_2vBXPQuZIfbrZkxLllzKUYlYeRQpfwkdSnmnHDS-xR2Jh00A31sQm_0sQl9bEKD0LWJCr158F_sDv0j8i_6Knh3EmD98j5g0jUpnGs8IaEr2sfwP_-_Mm-byg</recordid><startdate>20220514</startdate><enddate>20220514</enddate><creator>Qin, Caihua</creator><creator>Fang, Qianqian</creator><creator>Fan, Xing</creator><creator>Chen, Min</creator><creator>Jiang, Mingyi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6782-0394</orcidid></search><sort><creationdate>20220514</creationdate><title>Phosphorylation of DUF1639 protein by osmotic stress/ABA-activated protein kinase 10 regulates abscisic acid-induced antioxidant defense in rice</title><author>Qin, Caihua ; Fang, Qianqian ; Fan, Xing ; Chen, Min ; Jiang, Mingyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-2aae2a49fb835e40dce8e3a657460d17d7207e530cb9d3fd7552edbfb00d65893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abscisic Acid - metabolism</topic><topic>Antioxidant defense</topic><topic>Antioxidants - metabolism</topic><topic>Drought stress</topic><topic>Droughts</topic><topic>Gene Expression Regulation, Plant</topic><topic>Oryza - metabolism</topic><topic>OsDUF1639.1</topic><topic>Osmotic Pressure</topic><topic>Phosphorylation</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein Kinases - metabolism</topic><topic>SAPK10</topic><topic>Stress, Physiological</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qin, Caihua</creatorcontrib><creatorcontrib>Fang, Qianqian</creatorcontrib><creatorcontrib>Fan, Xing</creatorcontrib><creatorcontrib>Chen, Min</creatorcontrib><creatorcontrib>Jiang, Mingyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qin, Caihua</au><au>Fang, Qianqian</au><au>Fan, Xing</au><au>Chen, Min</au><au>Jiang, Mingyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of DUF1639 protein by osmotic stress/ABA-activated protein kinase 10 regulates abscisic acid-induced antioxidant defense in rice</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2022-05-14</date><risdate>2022</risdate><volume>604</volume><spage>30</spage><epage>36</epage><pages>30-36</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In rice (Oryza Sativa), Osmotic Stress/ABA-activated Protein Kinase 10 (SAPK10) has been shown to be induced by hyperosmotic stress and abscisic acid (ABA). However, the molecular function of SAPK10 and its downstream targets in ABA-induced antioxidant defense is poorly understood. Here, we identified an unknown function DUF1639 family protein, OsDUF1639.1, which interacts with SAPK10 in vitro and in vivo. OsDUF1639.1 positively regulates ABA responses in seed germination and tolerance to drought stress. We found that SAPK10 directly phosphorylates OsDUF1639.1 at Thr-80 in vitro. The transient expression analysis in combination with mutant analysis in rice protoplasts showed that Thr-80 is essential for ABA-induced stimulation of antioxidant defense by SAPK10. These results suggest that SAPK10 functions upstream of OsDUF1639.1 to regulate the activities of antioxidant enzymes, and Thr-80 phosphorylation of OsDUF1639.1 has a crucial role in ABA-induced antioxidant defense.
•OsDUF1639.1 positively regulates the ABA responses and tolerance to drought stress in rice.•OsDUF1639.1 interacting with SAPK10 affects ABA induced antioxidant defense.•Thr-80 phosphorylation of OsDUF1639.1 by SAPK10 is required for the effect on ABA-induced antioxidant defense.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>35279443</pmid><doi>10.1016/j.bbrc.2022.03.003</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6782-0394</orcidid></addata></record> |
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| subjects | Abscisic Acid - metabolism Antioxidant defense Antioxidants - metabolism Drought stress Droughts Gene Expression Regulation, Plant Oryza - metabolism OsDUF1639.1 Osmotic Pressure Phosphorylation Plant Proteins - genetics Plant Proteins - metabolism Protein Kinases - metabolism SAPK10 Stress, Physiological |
| title | Phosphorylation of DUF1639 protein by osmotic stress/ABA-activated protein kinase 10 regulates abscisic acid-induced antioxidant defense in rice |
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