Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker
Bladder cancer (BC) is one of the most common cancers in the world, with high morbidity and mortality. It is essential to develop a non-invasive, highly accurate, and simple method for BC diagnosis. This work proposed a fluorescent biosensor based on inorganic nanoflares combined with a DNAzyme walk...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2022-03, Vol.94 (11), p.4787-4793 |
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| creator | Zhang, Xiao Wei, Xiaowei Qi, Jijin Shen, Jie Xu, Junwen Gong, Guangyu Wei, Yong Yang, Jian Zhu, Qingyi Bai, Tingting Guo, Zhirui Qu, Xiaojun Zhu, Yefei |
| description | Bladder cancer (BC) is one of the most common cancers in the world, with high morbidity and mortality. It is essential to develop a non-invasive, highly accurate, and simple method for BC diagnosis. This work proposed a fluorescent biosensor based on inorganic nanoflares combined with a DNAzyme walker for the simultaneous detection of BC exosomal microRNAs (miRNAs). This biosensor was constructed on the Au nanoparticle (AuNP) modified with the carbon dot (CD)-labeled substrates and DNAzyme strands (AuNP@CDs inorganic nanoflares-DNAzyme, APCD). In the presence of target miRNAs, DNAzyme was activated and then cleaved the CD-labeled substrates and automatically walked along the AuNP, allowing fluorescence recovery. Due to the structure and functional composition, the APCD biosensors demonstrated high sensitivity and specificity, with the reached limit of detection for a single miRNA at the femtomolar level and wide linear range from 50 fM to 10 nM. Furthermore, the simultaneous analysis of BC-related exosomal miR-133b and miR-135b in clinical serum specimens was achieved and consistent with qRT-PCR, suggesting it is a potential method for the diagnosis of BC and other cancers. |
| doi_str_mv | 10.1021/acs.analchem.1c05588 |
| format | Article |
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It is essential to develop a non-invasive, highly accurate, and simple method for BC diagnosis. This work proposed a fluorescent biosensor based on inorganic nanoflares combined with a DNAzyme walker for the simultaneous detection of BC exosomal microRNAs (miRNAs). This biosensor was constructed on the Au nanoparticle (AuNP) modified with the carbon dot (CD)-labeled substrates and DNAzyme strands (AuNP@CDs inorganic nanoflares-DNAzyme, APCD). In the presence of target miRNAs, DNAzyme was activated and then cleaved the CD-labeled substrates and automatically walked along the AuNP, allowing fluorescence recovery. Due to the structure and functional composition, the APCD biosensors demonstrated high sensitivity and specificity, with the reached limit of detection for a single miRNA at the femtomolar level and wide linear range from 50 fM to 10 nM. Furthermore, the simultaneous analysis of BC-related exosomal miR-133b and miR-135b in clinical serum specimens was achieved and consistent with qRT-PCR, suggesting it is a potential method for the diagnosis of BC and other cancers.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.1c05588</identifier><identifier>PMID: 35275615</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biosensing Techniques - methods ; Biosensors ; Bladder ; Bladder cancer ; Cancer ; Chemistry ; Diagnosis ; DNA, Catalytic - chemistry ; Fluorescence ; Gold ; Gold - chemistry ; Humans ; Limit of Detection ; Metal Nanoparticles - chemistry ; MicroRNAs ; MicroRNAs - analysis ; miRNA ; Morbidity ; Nanoparticles ; Substrates ; Urinary Bladder Neoplasms - diagnosis ; Urinary Bladder Neoplasms - genetics</subject><ispartof>Analytical chemistry (Washington), 2022-03, Vol.94 (11), p.4787-4793</ispartof><rights>2022 American Chemical Society</rights><rights>Copyright American Chemical Society Mar 22, 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-1bab53276ae79d3fbbd918db78f701bf7a190e72b1aef16b48d08d23257b01a13</citedby><cites>FETCH-LOGICAL-a376t-1bab53276ae79d3fbbd918db78f701bf7a190e72b1aef16b48d08d23257b01a13</cites><orcidid>0000-0003-0332-7159 ; 0000-0003-2157-505X ; 0000-0002-5849-1469 ; 0000-0002-2625-2199</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c05588$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.1c05588$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35275615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xiao</creatorcontrib><creatorcontrib>Wei, Xiaowei</creatorcontrib><creatorcontrib>Qi, Jijin</creatorcontrib><creatorcontrib>Shen, Jie</creatorcontrib><creatorcontrib>Xu, Junwen</creatorcontrib><creatorcontrib>Gong, Guangyu</creatorcontrib><creatorcontrib>Wei, Yong</creatorcontrib><creatorcontrib>Yang, Jian</creatorcontrib><creatorcontrib>Zhu, Qingyi</creatorcontrib><creatorcontrib>Bai, Tingting</creatorcontrib><creatorcontrib>Guo, Zhirui</creatorcontrib><creatorcontrib>Qu, Xiaojun</creatorcontrib><creatorcontrib>Zhu, Yefei</creatorcontrib><title>Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Bladder cancer (BC) is one of the most common cancers in the world, with high morbidity and mortality. It is essential to develop a non-invasive, highly accurate, and simple method for BC diagnosis. This work proposed a fluorescent biosensor based on inorganic nanoflares combined with a DNAzyme walker for the simultaneous detection of BC exosomal microRNAs (miRNAs). This biosensor was constructed on the Au nanoparticle (AuNP) modified with the carbon dot (CD)-labeled substrates and DNAzyme strands (AuNP@CDs inorganic nanoflares-DNAzyme, APCD). In the presence of target miRNAs, DNAzyme was activated and then cleaved the CD-labeled substrates and automatically walked along the AuNP, allowing fluorescence recovery. Due to the structure and functional composition, the APCD biosensors demonstrated high sensitivity and specificity, with the reached limit of detection for a single miRNA at the femtomolar level and wide linear range from 50 fM to 10 nM. Furthermore, the simultaneous analysis of BC-related exosomal miR-133b and miR-135b in clinical serum specimens was achieved and consistent with qRT-PCR, suggesting it is a potential method for the diagnosis of BC and other cancers.</description><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Bladder</subject><subject>Bladder cancer</subject><subject>Cancer</subject><subject>Chemistry</subject><subject>Diagnosis</subject><subject>DNA, Catalytic - chemistry</subject><subject>Fluorescence</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>MicroRNAs</subject><subject>MicroRNAs - analysis</subject><subject>miRNA</subject><subject>Morbidity</subject><subject>Nanoparticles</subject><subject>Substrates</subject><subject>Urinary Bladder Neoplasms - diagnosis</subject><subject>Urinary Bladder Neoplasms - genetics</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtvFDEQhC0EIkvgHyBkiQuXWbrt9WOPm02ASGGReIjjqD32wCQz42DPSIRfj1e7yYEDp7p8Va3qYuwlwhJB4Ftq8pJG6pufYVhiA0pZ-4gtUAmotLXiMVsAgKyEAThhz3K-BkAE1E_ZiVTCKI1qwW6-dMPcTzSGOGd-HqbQTF0ceWz5WU_eh8S3NDZFLn7HHAfq-ceuSfHzbpP5GeXgeaEvx5h-0Ng1fEdjbHtKgdPo-flu8-duCPw79TchPWdPWupzeHHUU_bt3cXX7Yfq6tP7y-3mqiJp9FShI6ekMJqCWXvZOufXaL0ztjWArjWEawhGOKTQonYr68F6IYUyDpBQnrI3h9zbFH_NIU_10OUm9P2hZS20tAalllDQ1_-g13FO5at7Sgm0uNK6UKsDVYrnnEJb36ZuoHRXI9T7MeoyRn0_Rn0co9heHcNnNwT_YLr_fgHgAOztD4f_m_kXxsKYhA</recordid><startdate>20220322</startdate><enddate>20220322</enddate><creator>Zhang, Xiao</creator><creator>Wei, Xiaowei</creator><creator>Qi, Jijin</creator><creator>Shen, Jie</creator><creator>Xu, Junwen</creator><creator>Gong, Guangyu</creator><creator>Wei, Yong</creator><creator>Yang, Jian</creator><creator>Zhu, Qingyi</creator><creator>Bai, Tingting</creator><creator>Guo, Zhirui</creator><creator>Qu, Xiaojun</creator><creator>Zhu, Yefei</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0332-7159</orcidid><orcidid>https://orcid.org/0000-0003-2157-505X</orcidid><orcidid>https://orcid.org/0000-0002-5849-1469</orcidid><orcidid>https://orcid.org/0000-0002-2625-2199</orcidid></search><sort><creationdate>20220322</creationdate><title>Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker</title><author>Zhang, Xiao ; Wei, Xiaowei ; Qi, Jijin ; Shen, Jie ; Xu, Junwen ; Gong, Guangyu ; Wei, Yong ; Yang, Jian ; Zhu, Qingyi ; Bai, Tingting ; Guo, Zhirui ; Qu, Xiaojun ; Zhu, Yefei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-1bab53276ae79d3fbbd918db78f701bf7a190e72b1aef16b48d08d23257b01a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Bladder</topic><topic>Bladder cancer</topic><topic>Cancer</topic><topic>Chemistry</topic><topic>Diagnosis</topic><topic>DNA, Catalytic - chemistry</topic><topic>Fluorescence</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles - chemistry</topic><topic>MicroRNAs</topic><topic>MicroRNAs - analysis</topic><topic>miRNA</topic><topic>Morbidity</topic><topic>Nanoparticles</topic><topic>Substrates</topic><topic>Urinary Bladder Neoplasms - diagnosis</topic><topic>Urinary Bladder Neoplasms - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xiao</creatorcontrib><creatorcontrib>Wei, Xiaowei</creatorcontrib><creatorcontrib>Qi, Jijin</creatorcontrib><creatorcontrib>Shen, Jie</creatorcontrib><creatorcontrib>Xu, Junwen</creatorcontrib><creatorcontrib>Gong, Guangyu</creatorcontrib><creatorcontrib>Wei, Yong</creatorcontrib><creatorcontrib>Yang, Jian</creatorcontrib><creatorcontrib>Zhu, Qingyi</creatorcontrib><creatorcontrib>Bai, Tingting</creatorcontrib><creatorcontrib>Guo, Zhirui</creatorcontrib><creatorcontrib>Qu, Xiaojun</creatorcontrib><creatorcontrib>Zhu, Yefei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xiao</au><au>Wei, Xiaowei</au><au>Qi, Jijin</au><au>Shen, Jie</au><au>Xu, Junwen</au><au>Gong, Guangyu</au><au>Wei, Yong</au><au>Yang, Jian</au><au>Zhu, Qingyi</au><au>Bai, Tingting</au><au>Guo, Zhirui</au><au>Qu, Xiaojun</au><au>Zhu, Yefei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2022-03-22</date><risdate>2022</risdate><volume>94</volume><issue>11</issue><spage>4787</spage><epage>4793</epage><pages>4787-4793</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Bladder cancer (BC) is one of the most common cancers in the world, with high morbidity and mortality. It is essential to develop a non-invasive, highly accurate, and simple method for BC diagnosis. This work proposed a fluorescent biosensor based on inorganic nanoflares combined with a DNAzyme walker for the simultaneous detection of BC exosomal microRNAs (miRNAs). This biosensor was constructed on the Au nanoparticle (AuNP) modified with the carbon dot (CD)-labeled substrates and DNAzyme strands (AuNP@CDs inorganic nanoflares-DNAzyme, APCD). In the presence of target miRNAs, DNAzyme was activated and then cleaved the CD-labeled substrates and automatically walked along the AuNP, allowing fluorescence recovery. Due to the structure and functional composition, the APCD biosensors demonstrated high sensitivity and specificity, with the reached limit of detection for a single miRNA at the femtomolar level and wide linear range from 50 fM to 10 nM. Furthermore, the simultaneous analysis of BC-related exosomal miR-133b and miR-135b in clinical serum specimens was achieved and consistent with qRT-PCR, suggesting it is a potential method for the diagnosis of BC and other cancers.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>35275615</pmid><doi>10.1021/acs.analchem.1c05588</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0332-7159</orcidid><orcidid>https://orcid.org/0000-0003-2157-505X</orcidid><orcidid>https://orcid.org/0000-0002-5849-1469</orcidid><orcidid>https://orcid.org/0000-0002-2625-2199</orcidid></addata></record> |
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| subjects | Biosensing Techniques - methods Biosensors Bladder Bladder cancer Cancer Chemistry Diagnosis DNA, Catalytic - chemistry Fluorescence Gold Gold - chemistry Humans Limit of Detection Metal Nanoparticles - chemistry MicroRNAs MicroRNAs - analysis miRNA Morbidity Nanoparticles Substrates Urinary Bladder Neoplasms - diagnosis Urinary Bladder Neoplasms - genetics |
| title | Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker |
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