Long noncoding RNA LBX2‐AS1 promotes colorectal cancer progression via binding with PTBP1 and stabilizing KAT2A expression
The long noncoding RNAs (lncRNAs) have been investigated in colorectal cancer (CRC). The aim of this study is to identify the biological functions of LBX2‐AS1 in CRC. Quantitative real‐time polymerase chain reaction was used to examine the expression of LBX2‐AS1 in CRC cells. Cell counting kit‐8 and...
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| Veröffentlicht in: | Journal of biochemical and molecular toxicology 2022-05, Vol.36 (5), p.e23020-n/a |
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| creator | Zhao, Andong Wang, Yu Lin, Fuliang Bai, Keyun Gu, Chao |
| description | The long noncoding RNAs (lncRNAs) have been investigated in colorectal cancer (CRC). The aim of this study is to identify the biological functions of LBX2‐AS1 in CRC. Quantitative real‐time polymerase chain reaction was used to examine the expression of LBX2‐AS1 in CRC cells. Cell counting kit‐8 and colony formation assays were performed to examine cell proliferation. Wound healing and transwell invasion assays were performed to examine the cell migration and invasion. The interaction between PTBP1 and LBX2‐AS1 or KAT2A was confirmed by RNA immunoprecipitation. The KAT2A messenger RNA (mRNA) stability was probed using the transcriptional inhibitor Actinomycin D. LBX2‐AS1 was significantly increased in CRC tissues and cells. Knockdown of LBX2‐AS1 inhibited CRC cell proliferation, migration, and invasion. The notch signaling pathway was activated by LBX2‐AS1. LBX2‐AS1 enhanced the mRNA stability of the histone acetyltransferase KAT2A by interacting with RNA‐binding protein PTBP1. LBX2‐AS1 acted as an oncogene in CRC. |
| doi_str_mv | 10.1002/jbt.23020 |
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The aim of this study is to identify the biological functions of LBX2‐AS1 in CRC. Quantitative real‐time polymerase chain reaction was used to examine the expression of LBX2‐AS1 in CRC cells. Cell counting kit‐8 and colony formation assays were performed to examine cell proliferation. Wound healing and transwell invasion assays were performed to examine the cell migration and invasion. The interaction between PTBP1 and LBX2‐AS1 or KAT2A was confirmed by RNA immunoprecipitation. The KAT2A messenger RNA (mRNA) stability was probed using the transcriptional inhibitor Actinomycin D. LBX2‐AS1 was significantly increased in CRC tissues and cells. Knockdown of LBX2‐AS1 inhibited CRC cell proliferation, migration, and invasion. The notch signaling pathway was activated by LBX2‐AS1. LBX2‐AS1 enhanced the mRNA stability of the histone acetyltransferase KAT2A by interacting with RNA‐binding protein PTBP1. LBX2‐AS1 acted as an oncogene in CRC.</description><identifier>ISSN: 1095-6670</identifier><identifier>EISSN: 1099-0461</identifier><identifier>DOI: 10.1002/jbt.23020</identifier><identifier>PMID: 35253306</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Actinomycin ; Cancer ; Cell growth ; Cell Line, Tumor ; Cell migration ; Cell Movement ; Cell Proliferation ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - metabolism ; Gene Expression Regulation, Neoplastic ; Heterogeneous-Nuclear Ribonucleoproteins - genetics ; Heterogeneous-Nuclear Ribonucleoproteins - metabolism ; Histone acetyltransferase ; Histone Acetyltransferases - genetics ; Histone Acetyltransferases - metabolism ; Homeodomain Proteins - genetics ; Humans ; Immunoprecipitation ; KAT2A ; long noncoding RNA LBX2‐AS1 ; MicroRNAs - metabolism ; mRNA stability ; Polymerase chain reaction ; Polypyrimidine Tract-Binding Protein - genetics ; Polypyrimidine Tract-Binding Protein - metabolism ; PTBP1 ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; RNA-binding protein ; Signal transduction ; Stability ; Wound healing</subject><ispartof>Journal of biochemical and molecular toxicology, 2022-05, Vol.36 (5), p.e23020-n/a</ispartof><rights>2022 Wiley Periodicals LLC</rights><rights>2022 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-34b50b437dbf63765fe8e2bc2e4f3170f546b15950158ba8280917730e3726ba3</citedby><cites>FETCH-LOGICAL-c3530-34b50b437dbf63765fe8e2bc2e4f3170f546b15950158ba8280917730e3726ba3</cites><orcidid>0000-0002-7128-8721</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbt.23020$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbt.23020$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35253306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Andong</creatorcontrib><creatorcontrib>Wang, Yu</creatorcontrib><creatorcontrib>Lin, Fuliang</creatorcontrib><creatorcontrib>Bai, Keyun</creatorcontrib><creatorcontrib>Gu, Chao</creatorcontrib><title>Long noncoding RNA LBX2‐AS1 promotes colorectal cancer progression via binding with PTBP1 and stabilizing KAT2A expression</title><title>Journal of biochemical and molecular toxicology</title><addtitle>J Biochem Mol Toxicol</addtitle><description>The long noncoding RNAs (lncRNAs) have been investigated in colorectal cancer (CRC). The aim of this study is to identify the biological functions of LBX2‐AS1 in CRC. Quantitative real‐time polymerase chain reaction was used to examine the expression of LBX2‐AS1 in CRC cells. Cell counting kit‐8 and colony formation assays were performed to examine cell proliferation. Wound healing and transwell invasion assays were performed to examine the cell migration and invasion. The interaction between PTBP1 and LBX2‐AS1 or KAT2A was confirmed by RNA immunoprecipitation. The KAT2A messenger RNA (mRNA) stability was probed using the transcriptional inhibitor Actinomycin D. LBX2‐AS1 was significantly increased in CRC tissues and cells. Knockdown of LBX2‐AS1 inhibited CRC cell proliferation, migration, and invasion. The notch signaling pathway was activated by LBX2‐AS1. LBX2‐AS1 enhanced the mRNA stability of the histone acetyltransferase KAT2A by interacting with RNA‐binding protein PTBP1. LBX2‐AS1 acted as an oncogene in CRC.</description><subject>Actinomycin</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Heterogeneous-Nuclear Ribonucleoproteins - genetics</subject><subject>Heterogeneous-Nuclear Ribonucleoproteins - metabolism</subject><subject>Histone acetyltransferase</subject><subject>Histone Acetyltransferases - genetics</subject><subject>Histone Acetyltransferases - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>KAT2A</subject><subject>long noncoding RNA LBX2‐AS1</subject><subject>MicroRNAs - metabolism</subject><subject>mRNA stability</subject><subject>Polymerase chain reaction</subject><subject>Polypyrimidine Tract-Binding Protein - genetics</subject><subject>Polypyrimidine Tract-Binding Protein - metabolism</subject><subject>PTBP1</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>RNA-binding protein</subject><subject>Signal transduction</subject><subject>Stability</subject><subject>Wound healing</subject><issn>1095-6670</issn><issn>1099-0461</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctq3DAUhkVpaNK0i75AEXTTLpwcSZZkLz1Dcx3S0E4hOyNp5FSDR5pIntzIIo-QZ8yTxHNJF4Wuzg_nOx8HfoQ-EdgjAHR_qrs9yoDCG7RDoCwzyAV5u8o8E0LCNnqf0hQAeCn5O7TNOOWMgdhBD6PgL7EP3oSJ69PPswqPBhf0-fGp-kXwPIZZ6GzCJrQhWtOpFhvljY3L1WW0Kbng8bVTWDu_Mty47g8-Hw_OCVZ-glOntGvd_XJ1Wo1phe3tfHP3AW01qk3242buot8H38fDo2z04_B4WI0ywziDjOWag86ZnOhGMCl4YwtLtaE2bxiR0PBcaMJLDoQXWhW0gJJIycAySYVWbBd9XXv7n68WNnX1zCVj21Z5GxappoKJQgoBvEe__INOwyL6_rueEqyUAuSS-ramTAwpRdvU8-hmKt7VBOplJXVfSb2qpGc_b4wLPbOTv-RrBz2wvwZuXGvv_m-qTwbjtfIFIROUAA</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Zhao, Andong</creator><creator>Wang, Yu</creator><creator>Lin, Fuliang</creator><creator>Bai, Keyun</creator><creator>Gu, Chao</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7128-8721</orcidid></search><sort><creationdate>202205</creationdate><title>Long noncoding RNA LBX2‐AS1 promotes colorectal cancer progression via binding with PTBP1 and stabilizing KAT2A expression</title><author>Zhao, Andong ; Wang, Yu ; Lin, Fuliang ; Bai, Keyun ; Gu, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-34b50b437dbf63765fe8e2bc2e4f3170f546b15950158ba8280917730e3726ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Actinomycin</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Heterogeneous-Nuclear Ribonucleoproteins - genetics</topic><topic>Heterogeneous-Nuclear Ribonucleoproteins - metabolism</topic><topic>Histone acetyltransferase</topic><topic>Histone Acetyltransferases - genetics</topic><topic>Histone Acetyltransferases - metabolism</topic><topic>Homeodomain Proteins - genetics</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>KAT2A</topic><topic>long noncoding RNA LBX2‐AS1</topic><topic>MicroRNAs - metabolism</topic><topic>mRNA stability</topic><topic>Polymerase chain reaction</topic><topic>Polypyrimidine Tract-Binding Protein - genetics</topic><topic>Polypyrimidine Tract-Binding Protein - metabolism</topic><topic>PTBP1</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>RNA-binding protein</topic><topic>Signal transduction</topic><topic>Stability</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Andong</creatorcontrib><creatorcontrib>Wang, Yu</creatorcontrib><creatorcontrib>Lin, Fuliang</creatorcontrib><creatorcontrib>Bai, Keyun</creatorcontrib><creatorcontrib>Gu, Chao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and molecular toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Andong</au><au>Wang, Yu</au><au>Lin, Fuliang</au><au>Bai, Keyun</au><au>Gu, Chao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long noncoding RNA LBX2‐AS1 promotes colorectal cancer progression via binding with PTBP1 and stabilizing KAT2A expression</atitle><jtitle>Journal of biochemical and molecular toxicology</jtitle><addtitle>J Biochem Mol Toxicol</addtitle><date>2022-05</date><risdate>2022</risdate><volume>36</volume><issue>5</issue><spage>e23020</spage><epage>n/a</epage><pages>e23020-n/a</pages><issn>1095-6670</issn><eissn>1099-0461</eissn><abstract>The long noncoding RNAs (lncRNAs) have been investigated in colorectal cancer (CRC). The aim of this study is to identify the biological functions of LBX2‐AS1 in CRC. Quantitative real‐time polymerase chain reaction was used to examine the expression of LBX2‐AS1 in CRC cells. Cell counting kit‐8 and colony formation assays were performed to examine cell proliferation. Wound healing and transwell invasion assays were performed to examine the cell migration and invasion. The interaction between PTBP1 and LBX2‐AS1 or KAT2A was confirmed by RNA immunoprecipitation. The KAT2A messenger RNA (mRNA) stability was probed using the transcriptional inhibitor Actinomycin D. LBX2‐AS1 was significantly increased in CRC tissues and cells. Knockdown of LBX2‐AS1 inhibited CRC cell proliferation, migration, and invasion. The notch signaling pathway was activated by LBX2‐AS1. LBX2‐AS1 enhanced the mRNA stability of the histone acetyltransferase KAT2A by interacting with RNA‐binding protein PTBP1. LBX2‐AS1 acted as an oncogene in CRC.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35253306</pmid><doi>10.1002/jbt.23020</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0002-7128-8721</orcidid></addata></record> |
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| subjects | Actinomycin Cancer Cell growth Cell Line, Tumor Cell migration Cell Movement Cell Proliferation Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Gene Expression Regulation, Neoplastic Heterogeneous-Nuclear Ribonucleoproteins - genetics Heterogeneous-Nuclear Ribonucleoproteins - metabolism Histone acetyltransferase Histone Acetyltransferases - genetics Histone Acetyltransferases - metabolism Homeodomain Proteins - genetics Humans Immunoprecipitation KAT2A long noncoding RNA LBX2‐AS1 MicroRNAs - metabolism mRNA stability Polymerase chain reaction Polypyrimidine Tract-Binding Protein - genetics Polypyrimidine Tract-Binding Protein - metabolism PTBP1 RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism RNA-binding protein Signal transduction Stability Wound healing |
| title | Long noncoding RNA LBX2‐AS1 promotes colorectal cancer progression via binding with PTBP1 and stabilizing KAT2A expression |
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