Rapid detection of trace Salmonella in milk using an effective pretreatment combined with droplet digital polymerase chain reaction

•A pretreatment is proposed based on in situ culture and immunomagnetic separation.•The 25 mL large system is adopted for Immunomagnetic separation.•The pretreatment method with ddPCR could detect 5 CFU/25 mL Salmonella in milk. Salmonella is one of the most dangerous food-borne pathogens around the...

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Veröffentlicht in:	Microbiological research 2021-10, Vol.251, p.126838-126838, Article 126838
Hauptverfasser:	Du, Meihong, Li, Jingwen, Liu, Qingjun, Wang, Yanfei, Chen, Erning, Kang, Fuying, Tu, Chenxiao
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Droplet digital polymerase chain reaction (ddPCR) droplets enrichment culture Food Microbiology - methods Immunomagnetic separation Limit of Detection milk Milk - microbiology Polymerase Chain Reaction Pretreatment Rapid detection rapid methods Salmonella Salmonella - genetics Salmonella - isolation & purification
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