An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing

•Improved viral mapping single-stranded DNA viruses by >60-fold for begomoviruses out of plants.•Successful sequencing of viral single-stranded DNA from whiteflies over a wide range of viral loads.•Size selection improved viral read mapping by over 90 %. The ability of begomoviruses to evolve rap...

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