A Tyrosine Phosphoproteome Analysis Approach Enabled by Selective Dephosphorylation with Protein Tyrosine Phosphatase

Protein tyrosine phosphorylation (pTyr) plays a prominent role in signal transduction and regulation in all eukaryotic cells. In conventional immunoaffinity purification (IP) methods, phosphotyrosine peptides are isolated from the digest of cellular protein extracts with a phosphotyrosine-specific a...

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Veröffentlicht in:	Analytical chemistry (Washington) 2022-03, Vol.94 (10), p.4155-4164
Hauptverfasser:	Liu, Xiaoyan, Dong, Mingming, Yao, Yating, Wang, Yan, Mao, Jiawei, Hu, Lianghai, Yao, Lishan, Ye, Mingliang
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Chemistry Cost analysis Dephosphorylation Humans Lymphocytes Lymphocytes T Mass spectrometry Mass spectroscopy Peptides Peptides - chemistry Phosphatase Phosphorylation Phosphotyrosine Phosphotyrosine - chemistry Protein purification Protein Tyrosine Phosphatases Protein-tyrosine-phosphatase Proteins Proteome - analysis Proteomics - methods Reproducibility of Results SHP-1 protein Signal transduction Tyrosine Tyrosine - chemistry Workflow
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