Profile analysis of circRNAs in human THP-1 derived macrophages infected with intracellular Staphylococcus aureus
Intracellular Staphylococcus aureus (S. aureus) infection is generally persistent, recurrent and difficult to treat due to the poor availability of antibiotics within macrophages cells and the lack of ideal diagnostic markers. Circular RNAs (circRNAs), with covalently closed circular structures, exi...
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| Veröffentlicht in: | Microbial pathogenesis 2022-04, Vol.165, p.105466-105466, Article 105466 |
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| creator | Xie, Xiaolu Chen, Zhihao Han, Mingxiao Wang, Xi Wang, Min Lv, Jingnan Xie, Xiaofang Zhai, Yaxuan Li, Liubing Du, Hong Xie, Zonggang Zhang, Haifang |
| description | Intracellular Staphylococcus aureus (S. aureus) infection is generally persistent, recurrent and difficult to treat due to the poor availability of antibiotics within macrophages cells and the lack of ideal diagnostic markers. Circular RNAs (circRNAs), with covalently closed circular structures, exists in the serum stably and is not easily degraded by nucleases. Besides, circRNAs play a pivotal in the eukaryotic regulation of genes expression and served as biomarkers in variety disease including microbial infections. However, the function of host circRNAs in intracellular S. aureus infection remains largely unclear.
In this study, the circRNAs expression profile was investigated by RNA sequencing technology in both S. aureus-infected THP-1 derived macrophages and mock control cells. The differentially expressed circRNAs (DE circRNAs) with a fold-change >1.5 (p 1.5 (p |
| doi_str_mv | 10.1016/j.micpath.2022.105466 |
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In this study, the circRNAs expression profile was investigated by RNA sequencing technology in both S. aureus-infected THP-1 derived macrophages and mock control cells. The differentially expressed circRNAs (DE circRNAs) with a fold-change >1.5 (p < 0.05) are analyzed using functional pathway clustering prediction. Then, RT-qPCR was performed to verify the top 2 up-regulated circRNAs in the THP-1 cell and human serum samples so as to evaluate the value of circRNAs for S. aureus diagnosis.
An intracellular survival THP-1 derived macrophages model of S. aureus infection was established. A total of 5,299 circRNAs were identified in human THP-1 derived macrophages infected with intracellular S. aureus. There were 61 DE circRNAs with a fold-change >1.5 (p < 0.05) after S. aureus infection. Among them, 22 circRNAs were up-regulated while 39 circRNAs down-regulated. GO and KEGG pathway analysis demonstrated that DE circRNAs were enriched in the processes such as Neurotrophin, Pyruvate metabolism and Notch signaling pathway. Moreover, hsa_circ_0000311 and chr13:43500472–43544806-(novel) were verified to be significantly upregulated in THP-1 derived macrophages and human serum samples between two groups. Finally, the networks of circRNA-miRNA-mRNA based on these two circRNAs were constructed respectively.
Our study provides the first profile analysis of host circRNAs involved in intracellular S. aureus infection, which may serve as biomarkers for S. aureus diagnosis and contribute to the understanding of S. aureus evasion mechanisms.
•A cell model of intracellular S. aureus infection in human THP-1 derived macrophages is constructed.•CircRNAs may serve as biomarkers for S. aureus diagnosis.•CircRNAs are contribute to the understanding of S. aureus evasion mechanisms.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2022.105466</identifier><identifier>PMID: 35247499</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Biomarkers ; CircRNAs ; Humans ; Intracellular survival ; Macrophage ; Macrophages - metabolism ; MicroRNAs - genetics ; RNA, Circular - genetics ; RNA-Seq ; Staphylococcus aureus ; Staphylococcus aureus - genetics ; Staphylococcus aureus - metabolism</subject><ispartof>Microbial pathogenesis, 2022-04, Vol.165, p.105466-105466, Article 105466</ispartof><rights>2022 Elsevier Ltd</rights><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-25cb958b17d4f5809e49379e2b7d44aeee705ebe62e3bbda6eda4040700b340f3</citedby><cites>FETCH-LOGICAL-c365t-25cb958b17d4f5809e49379e2b7d44aeee705ebe62e3bbda6eda4040700b340f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2022.105466$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35247499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Xiaolu</creatorcontrib><creatorcontrib>Chen, Zhihao</creatorcontrib><creatorcontrib>Han, Mingxiao</creatorcontrib><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Lv, Jingnan</creatorcontrib><creatorcontrib>Xie, Xiaofang</creatorcontrib><creatorcontrib>Zhai, Yaxuan</creatorcontrib><creatorcontrib>Li, Liubing</creatorcontrib><creatorcontrib>Du, Hong</creatorcontrib><creatorcontrib>Xie, Zonggang</creatorcontrib><creatorcontrib>Zhang, Haifang</creatorcontrib><title>Profile analysis of circRNAs in human THP-1 derived macrophages infected with intracellular Staphylococcus aureus</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Intracellular Staphylococcus aureus (S. aureus) infection is generally persistent, recurrent and difficult to treat due to the poor availability of antibiotics within macrophages cells and the lack of ideal diagnostic markers. Circular RNAs (circRNAs), with covalently closed circular structures, exists in the serum stably and is not easily degraded by nucleases. Besides, circRNAs play a pivotal in the eukaryotic regulation of genes expression and served as biomarkers in variety disease including microbial infections. However, the function of host circRNAs in intracellular S. aureus infection remains largely unclear.
In this study, the circRNAs expression profile was investigated by RNA sequencing technology in both S. aureus-infected THP-1 derived macrophages and mock control cells. The differentially expressed circRNAs (DE circRNAs) with a fold-change >1.5 (p < 0.05) are analyzed using functional pathway clustering prediction. Then, RT-qPCR was performed to verify the top 2 up-regulated circRNAs in the THP-1 cell and human serum samples so as to evaluate the value of circRNAs for S. aureus diagnosis.
An intracellular survival THP-1 derived macrophages model of S. aureus infection was established. A total of 5,299 circRNAs were identified in human THP-1 derived macrophages infected with intracellular S. aureus. There were 61 DE circRNAs with a fold-change >1.5 (p < 0.05) after S. aureus infection. Among them, 22 circRNAs were up-regulated while 39 circRNAs down-regulated. GO and KEGG pathway analysis demonstrated that DE circRNAs were enriched in the processes such as Neurotrophin, Pyruvate metabolism and Notch signaling pathway. Moreover, hsa_circ_0000311 and chr13:43500472–43544806-(novel) were verified to be significantly upregulated in THP-1 derived macrophages and human serum samples between two groups. Finally, the networks of circRNA-miRNA-mRNA based on these two circRNAs were constructed respectively.
Our study provides the first profile analysis of host circRNAs involved in intracellular S. aureus infection, which may serve as biomarkers for S. aureus diagnosis and contribute to the understanding of S. aureus evasion mechanisms.
•A cell model of intracellular S. aureus infection in human THP-1 derived macrophages is constructed.•CircRNAs may serve as biomarkers for S. aureus diagnosis.•CircRNAs are contribute to the understanding of S. aureus evasion mechanisms.</description><subject>Biomarkers</subject><subject>CircRNAs</subject><subject>Humans</subject><subject>Intracellular survival</subject><subject>Macrophage</subject><subject>Macrophages - metabolism</subject><subject>MicroRNAs - genetics</subject><subject>RNA, Circular - genetics</subject><subject>RNA-Seq</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - metabolism</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v2zAMhoVhw5J2-wkbdNzFKSXLsnUagqBbCxRd0XZnQZbpWoG_ItkZ8u-nIGmvPRF68ZAUH0K-MVgxYPJqu-qcHc3UrDhwHrNMSPmBLBkomTAOxUeyhKLgiQAGC3IRwhYAlEjVZ7JIMy5yodSS7B78ULsWqelNewgu0KGm1nn7eL8O1PW0mTvT0-ebh4TRCr3bY0U7Y_0wNuYFj0iNdorhPzc18TV5Y7Ft59Z4-jSZsTm0gx2snQM1s8c5fCGfatMG_Hqul-Tvr-vnzU1y9-f37WZ9l9hUZlPCM1uqrChZXok6K0ChUGmukJcxEAYRc8iwRMkxLcvKSKyMAAE5QJkKqNNL8uM0d_TDbsYw6c6F49dMj8McNJepZILLvIhodkLjWSF4rPXoXWf8QTPQR9t6q8-29dG2PtmOfd_PK-ayw-qt61VvBH6eAIyH7h16HazD3mLlfLSmq8G9s-I_a7qU2Q</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Xie, Xiaolu</creator><creator>Chen, Zhihao</creator><creator>Han, Mingxiao</creator><creator>Wang, Xi</creator><creator>Wang, Min</creator><creator>Lv, Jingnan</creator><creator>Xie, Xiaofang</creator><creator>Zhai, Yaxuan</creator><creator>Li, Liubing</creator><creator>Du, Hong</creator><creator>Xie, Zonggang</creator><creator>Zhang, Haifang</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202204</creationdate><title>Profile analysis of circRNAs in human THP-1 derived macrophages infected with intracellular Staphylococcus aureus</title><author>Xie, Xiaolu ; Chen, Zhihao ; Han, Mingxiao ; Wang, Xi ; Wang, Min ; Lv, Jingnan ; Xie, Xiaofang ; Zhai, Yaxuan ; Li, Liubing ; Du, Hong ; Xie, Zonggang ; Zhang, Haifang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-25cb958b17d4f5809e49379e2b7d44aeee705ebe62e3bbda6eda4040700b340f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomarkers</topic><topic>CircRNAs</topic><topic>Humans</topic><topic>Intracellular survival</topic><topic>Macrophage</topic><topic>Macrophages - metabolism</topic><topic>MicroRNAs - genetics</topic><topic>RNA, Circular - genetics</topic><topic>RNA-Seq</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Xiaolu</creatorcontrib><creatorcontrib>Chen, Zhihao</creatorcontrib><creatorcontrib>Han, Mingxiao</creatorcontrib><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Lv, Jingnan</creatorcontrib><creatorcontrib>Xie, Xiaofang</creatorcontrib><creatorcontrib>Zhai, Yaxuan</creatorcontrib><creatorcontrib>Li, Liubing</creatorcontrib><creatorcontrib>Du, Hong</creatorcontrib><creatorcontrib>Xie, Zonggang</creatorcontrib><creatorcontrib>Zhang, Haifang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Xiaolu</au><au>Chen, Zhihao</au><au>Han, Mingxiao</au><au>Wang, Xi</au><au>Wang, Min</au><au>Lv, Jingnan</au><au>Xie, Xiaofang</au><au>Zhai, Yaxuan</au><au>Li, Liubing</au><au>Du, Hong</au><au>Xie, Zonggang</au><au>Zhang, Haifang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profile analysis of circRNAs in human THP-1 derived macrophages infected with intracellular Staphylococcus aureus</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2022-04</date><risdate>2022</risdate><volume>165</volume><spage>105466</spage><epage>105466</epage><pages>105466-105466</pages><artnum>105466</artnum><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Intracellular Staphylococcus aureus (S. aureus) infection is generally persistent, recurrent and difficult to treat due to the poor availability of antibiotics within macrophages cells and the lack of ideal diagnostic markers. Circular RNAs (circRNAs), with covalently closed circular structures, exists in the serum stably and is not easily degraded by nucleases. Besides, circRNAs play a pivotal in the eukaryotic regulation of genes expression and served as biomarkers in variety disease including microbial infections. However, the function of host circRNAs in intracellular S. aureus infection remains largely unclear.
In this study, the circRNAs expression profile was investigated by RNA sequencing technology in both S. aureus-infected THP-1 derived macrophages and mock control cells. The differentially expressed circRNAs (DE circRNAs) with a fold-change >1.5 (p < 0.05) are analyzed using functional pathway clustering prediction. Then, RT-qPCR was performed to verify the top 2 up-regulated circRNAs in the THP-1 cell and human serum samples so as to evaluate the value of circRNAs for S. aureus diagnosis.
An intracellular survival THP-1 derived macrophages model of S. aureus infection was established. A total of 5,299 circRNAs were identified in human THP-1 derived macrophages infected with intracellular S. aureus. There were 61 DE circRNAs with a fold-change >1.5 (p < 0.05) after S. aureus infection. Among them, 22 circRNAs were up-regulated while 39 circRNAs down-regulated. GO and KEGG pathway analysis demonstrated that DE circRNAs were enriched in the processes such as Neurotrophin, Pyruvate metabolism and Notch signaling pathway. Moreover, hsa_circ_0000311 and chr13:43500472–43544806-(novel) were verified to be significantly upregulated in THP-1 derived macrophages and human serum samples between two groups. Finally, the networks of circRNA-miRNA-mRNA based on these two circRNAs were constructed respectively.
Our study provides the first profile analysis of host circRNAs involved in intracellular S. aureus infection, which may serve as biomarkers for S. aureus diagnosis and contribute to the understanding of S. aureus evasion mechanisms.
•A cell model of intracellular S. aureus infection in human THP-1 derived macrophages is constructed.•CircRNAs may serve as biomarkers for S. aureus diagnosis.•CircRNAs are contribute to the understanding of S. aureus evasion mechanisms.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>35247499</pmid><doi>10.1016/j.micpath.2022.105466</doi><tpages>1</tpages></addata></record> |
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| subjects | Biomarkers CircRNAs Humans Intracellular survival Macrophage Macrophages - metabolism MicroRNAs - genetics RNA, Circular - genetics RNA-Seq Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - metabolism |
| title | Profile analysis of circRNAs in human THP-1 derived macrophages infected with intracellular Staphylococcus aureus |
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