Lack of leaf carbonic anhydrase activity eliminates the C4 carbon‐concentrating mechanism requiring direct diffusion of CO2 into bundle sheath cells

Carbonic anhydrase (CA) performs the first enzymatic step of C4 photosynthesis by catalysing the reversible hydration of dissolved CO2 that diffuses into mesophyll cells from intercellular airspaces. This CA‐catalysed reaction provides the bicarbonate used by phosphoenolpyruvate carboxylase to gener...

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