Fine mapping of Pm58 from Aegilops tauschii conferring powdery mildew resistance

Key message The powdery mildew resistance gene Pm58 was traced to a 141.3-kb interval with the co-segregating marker Xkasp68500 in wheat breeding. Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58...

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Veröffentlicht in:	Theoretical and applied genetics 2022-05, Vol.135 (5), p.1657-1669
Hauptverfasser:	Xue, Shulin, Hu, Shanshan, Chen, Xian, Ma, Yuyu, Lu, Mingxue, Bai, Shenglong, Wang, Xintian, Sun, Tiepeng, Wang, Yingxue, Wan, Hongshen, An, Xia, Li, Suoping
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Sprache:	eng
Schlagworte:	Aegilops - genetics Aegilops tauschii Agricultural research Agriculture Biochemistry Biomedical and Life Sciences Biotechnology Chromosome Mapping Control Cultivars Disease resistance Disease Resistance - genetics Diseases and pests Gene mapping Genes, Plant Genetic aspects Genetic Markers Genomes Genotyping Goat grass Grasses Haplotypes Humans Life Sciences Methods Nucleotide sequence Original Article Phenotyping Plant Biochemistry Plant Breeding Plant Breeding/Biotechnology Plant Diseases - genetics Plant Genetics and Genomics Plant immunology Powdery mildew Powdery mildew diseases Quantitative trait loci Recombinants Triticum - genetics Triticum aestivum Wheat
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container_issue	5
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container_title	Theoretical and applied genetics
container_volume	135
creator	Xue, Shulin Hu, Shanshan Chen, Xian Ma, Yuyu Lu, Mingxue Bai, Shenglong Wang, Xintian Sun, Tiepeng Wang, Yingxue Wan, Hongshen An, Xia Li, Suoping
description	Key message The powdery mildew resistance gene Pm58 was traced to a 141.3-kb interval with the co-segregating marker Xkasp68500 in wheat breeding. Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58 , an F 2 population of 676 plants derived from the cross T093 × TA1662 was used for recombinant screening. We obtained 13 recombinants that occurred between the flanking markers Xhnu670 and Xhnu186 . Genotyping and phenotyping these recombinant F 2:3 families delimited Pm58 to a 0.22-cM interval ( Xsts20220 – Xkasp61553 ) on chromosome arm 2DS. The region carrying the Pm58 locus was approximately 141.3-kb, which contained eight annotated genes according to the reference genome sequence of Ae. tauschii AL8/78. Haplotype analysis of 178 Ae. tauschii accessions using the candidate gene-specific markers identified a disease resistance gene AET2Gv20068500 as a candidate for Pm58. Comparative mapping of the Pm58- containing interval revealed two presence/absence variations (PAVs) between AL8/78 and common wheat Chinese Spring. PAV-1 resides in the 3′-end of AET2Gv20068500. The majority of 158 common wheat cultivars (84.8%) displayed the absence of a 14.1-kb fragment in the PAV-1 region, which was confirmed by aligning the targeted genome sequences of the other sequenced Ae. tauschii accessions and common wheat cultivars. A co-segregating marker Xkasp68500 developed from AET2Gv20068500 can distinguish TA1662 from all randomly selected common wheat cultivars and will be instrumental for tracking Pm58 in breeding programs.
doi_str_mv	10.1007/s00122-022-04061-8
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Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58 , an F 2 population of 676 plants derived from the cross T093 × TA1662 was used for recombinant screening. We obtained 13 recombinants that occurred between the flanking markers Xhnu670 and Xhnu186 . Genotyping and phenotyping these recombinant F 2:3 families delimited Pm58 to a 0.22-cM interval ( Xsts20220 – Xkasp61553 ) on chromosome arm 2DS. The region carrying the Pm58 locus was approximately 141.3-kb, which contained eight annotated genes according to the reference genome sequence of Ae. tauschii AL8/78. Haplotype analysis of 178 Ae. tauschii accessions using the candidate gene-specific markers identified a disease resistance gene AET2Gv20068500 as a candidate for Pm58. Comparative mapping of the Pm58- containing interval revealed two presence/absence variations (PAVs) between AL8/78 and common wheat Chinese Spring. PAV-1 resides in the 3′-end of AET2Gv20068500. The majority of 158 common wheat cultivars (84.8%) displayed the absence of a 14.1-kb fragment in the PAV-1 region, which was confirmed by aligning the targeted genome sequences of the other sequenced Ae. tauschii accessions and common wheat cultivars. A co-segregating marker Xkasp68500 developed from AET2Gv20068500 can distinguish TA1662 from all randomly selected common wheat cultivars and will be instrumental for tracking Pm58 in breeding programs.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-022-04061-8</identifier><identifier>PMID: 35234985</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aegilops - genetics ; Aegilops tauschii ; Agricultural research ; Agriculture ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Chromosome Mapping ; Control ; Cultivars ; Disease resistance ; Disease Resistance - genetics ; Diseases and pests ; Gene mapping ; Genes, Plant ; Genetic aspects ; Genetic Markers ; Genomes ; Genotyping ; Goat grass ; Grasses ; Haplotypes ; Humans ; Life Sciences ; Methods ; Nucleotide sequence ; Original Article ; Phenotyping ; Plant Biochemistry ; Plant Breeding ; Plant Breeding/Biotechnology ; Plant Diseases - genetics ; Plant Genetics and Genomics ; Plant immunology ; Powdery mildew ; Powdery mildew diseases ; Quantitative trait loci ; Recombinants ; Triticum - genetics ; Triticum aestivum ; Wheat</subject><ispartof>Theoretical and applied genetics, 2022-05, Vol.135 (5), p.1657-1669</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2022 Springer</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-8b5876a488ff5aea0a4d4a740bf65d04fbaff212092a85986f3f92bedfb755ec3</citedby><cites>FETCH-LOGICAL-c476t-8b5876a488ff5aea0a4d4a740bf65d04fbaff212092a85986f3f92bedfb755ec3</cites><orcidid>0000-0002-1531-9159</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00122-022-04061-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00122-022-04061-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35234985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Shulin</creatorcontrib><creatorcontrib>Hu, Shanshan</creatorcontrib><creatorcontrib>Chen, Xian</creatorcontrib><creatorcontrib>Ma, Yuyu</creatorcontrib><creatorcontrib>Lu, Mingxue</creatorcontrib><creatorcontrib>Bai, Shenglong</creatorcontrib><creatorcontrib>Wang, Xintian</creatorcontrib><creatorcontrib>Sun, Tiepeng</creatorcontrib><creatorcontrib>Wang, Yingxue</creatorcontrib><creatorcontrib>Wan, Hongshen</creatorcontrib><creatorcontrib>An, Xia</creatorcontrib><creatorcontrib>Li, Suoping</creatorcontrib><title>Fine mapping of Pm58 from Aegilops tauschii conferring powdery mildew resistance</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><addtitle>Theor Appl Genet</addtitle><description>Key message The powdery mildew resistance gene Pm58 was traced to a 141.3-kb interval with the co-segregating marker Xkasp68500 in wheat breeding. Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58 , an F 2 population of 676 plants derived from the cross T093 × TA1662 was used for recombinant screening. We obtained 13 recombinants that occurred between the flanking markers Xhnu670 and Xhnu186 . Genotyping and phenotyping these recombinant F 2:3 families delimited Pm58 to a 0.22-cM interval ( Xsts20220 – Xkasp61553 ) on chromosome arm 2DS. The region carrying the Pm58 locus was approximately 141.3-kb, which contained eight annotated genes according to the reference genome sequence of Ae. tauschii AL8/78. Haplotype analysis of 178 Ae. tauschii accessions using the candidate gene-specific markers identified a disease resistance gene AET2Gv20068500 as a candidate for Pm58. Comparative mapping of the Pm58- containing interval revealed two presence/absence variations (PAVs) between AL8/78 and common wheat Chinese Spring. PAV-1 resides in the 3′-end of AET2Gv20068500. The majority of 158 common wheat cultivars (84.8%) displayed the absence of a 14.1-kb fragment in the PAV-1 region, which was confirmed by aligning the targeted genome sequences of the other sequenced Ae. tauschii accessions and common wheat cultivars. A co-segregating marker Xkasp68500 developed from AET2Gv20068500 can distinguish TA1662 from all randomly selected common wheat cultivars and will be instrumental for tracking Pm58 in breeding programs.</description><subject>Aegilops - genetics</subject><subject>Aegilops tauschii</subject><subject>Agricultural research</subject><subject>Agriculture</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chromosome Mapping</subject><subject>Control</subject><subject>Cultivars</subject><subject>Disease resistance</subject><subject>Disease Resistance - genetics</subject><subject>Diseases and pests</subject><subject>Gene mapping</subject><subject>Genes, Plant</subject><subject>Genetic aspects</subject><subject>Genetic Markers</subject><subject>Genomes</subject><subject>Genotyping</subject><subject>Goat grass</subject><subject>Grasses</subject><subject>Haplotypes</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Nucleotide sequence</subject><subject>Original Article</subject><subject>Phenotyping</subject><subject>Plant Biochemistry</subject><subject>Plant Breeding</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Diseases - genetics</subject><subject>Plant Genetics and Genomics</subject><subject>Plant immunology</subject><subject>Powdery mildew</subject><subject>Powdery mildew diseases</subject><subject>Quantitative trait loci</subject><subject>Recombinants</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtr3TAQhUVJaW7T_oEsgiCbduF0rIctLy-haQOBhrRdC9ke3SjYliPZpPn3lbl5cEMIYhAM3xlmziHkMIeTHKD8FgFyxjJYSkCRZ-odWeWCs4wxwfbIClI7k6Vk--RjjDcAwCTwD2SfS8ZFpeSKXJ65AWlvxtENG-otveylojb4nq5x4zo_RjqZOTbXztHGDxZDWMjR37UY7mnvuhbvaMDo4mSGBj-R99Z0ET8__Afk79n3P6c_s4tfP85P1xdZI8piylQtVVkYoZS10qABI1phSgG1LWQLwtbGWpYzqJhRslKF5bZiNba2LqXEhh-QL9u5Y_C3M8ZJ9y422HVmQD9HzYp0ZPKCVwk9foHe-DkMabtEFaKSUiT6idqYDrUbrJ-CaZahel0mIyWXQibq5BUqvRZ7l_xB61J_R_B1R5CYCf9Nm-Rp1Oe_r3ZZtmWb4GMMaPUYXG_Cvc5BL5HrbeQalloi1yqJjh6um-se2yfJY8YJ4Fsgjkt0GJ7Pf2Psf_YLs3w</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Xue, Shulin</creator><creator>Hu, Shanshan</creator><creator>Chen, Xian</creator><creator>Ma, Yuyu</creator><creator>Lu, Mingxue</creator><creator>Bai, Shenglong</creator><creator>Wang, Xintian</creator><creator>Sun, Tiepeng</creator><creator>Wang, Yingxue</creator><creator>Wan, Hongshen</creator><creator>An, Xia</creator><creator>Li, Suoping</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1531-9159</orcidid></search><sort><creationdate>20220501</creationdate><title>Fine mapping of Pm58 from Aegilops tauschii conferring powdery mildew resistance</title><author>Xue, Shulin ; Hu, Shanshan ; Chen, Xian ; Ma, Yuyu ; Lu, Mingxue ; Bai, Shenglong ; Wang, Xintian ; Sun, Tiepeng ; Wang, Yingxue ; Wan, Hongshen ; An, Xia ; Li, Suoping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-8b5876a488ff5aea0a4d4a740bf65d04fbaff212092a85986f3f92bedfb755ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aegilops - genetics</topic><topic>Aegilops tauschii</topic><topic>Agricultural research</topic><topic>Agriculture</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Chromosome Mapping</topic><topic>Control</topic><topic>Cultivars</topic><topic>Disease resistance</topic><topic>Disease Resistance - genetics</topic><topic>Diseases and pests</topic><topic>Gene mapping</topic><topic>Genes, Plant</topic><topic>Genetic aspects</topic><topic>Genetic Markers</topic><topic>Genomes</topic><topic>Genotyping</topic><topic>Goat grass</topic><topic>Grasses</topic><topic>Haplotypes</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Methods</topic><topic>Nucleotide sequence</topic><topic>Original Article</topic><topic>Phenotyping</topic><topic>Plant Biochemistry</topic><topic>Plant Breeding</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Diseases - genetics</topic><topic>Plant Genetics and Genomics</topic><topic>Plant immunology</topic><topic>Powdery mildew</topic><topic>Powdery mildew diseases</topic><topic>Quantitative trait loci</topic><topic>Recombinants</topic><topic>Triticum - 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Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58 , an F 2 population of 676 plants derived from the cross T093 × TA1662 was used for recombinant screening. We obtained 13 recombinants that occurred between the flanking markers Xhnu670 and Xhnu186 . Genotyping and phenotyping these recombinant F 2:3 families delimited Pm58 to a 0.22-cM interval ( Xsts20220 – Xkasp61553 ) on chromosome arm 2DS. The region carrying the Pm58 locus was approximately 141.3-kb, which contained eight annotated genes according to the reference genome sequence of Ae. tauschii AL8/78. Haplotype analysis of 178 Ae. tauschii accessions using the candidate gene-specific markers identified a disease resistance gene AET2Gv20068500 as a candidate for Pm58. Comparative mapping of the Pm58- containing interval revealed two presence/absence variations (PAVs) between AL8/78 and common wheat Chinese Spring. PAV-1 resides in the 3′-end of AET2Gv20068500. The majority of 158 common wheat cultivars (84.8%) displayed the absence of a 14.1-kb fragment in the PAV-1 region, which was confirmed by aligning the targeted genome sequences of the other sequenced Ae. tauschii accessions and common wheat cultivars. A co-segregating marker Xkasp68500 developed from AET2Gv20068500 can distinguish TA1662 from all randomly selected common wheat cultivars and will be instrumental for tracking Pm58 in breeding programs.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35234985</pmid><doi>10.1007/s00122-022-04061-8</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-1531-9159</orcidid></addata></record>
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subjects	Aegilops - genetics Aegilops tauschii Agricultural research Agriculture Biochemistry Biomedical and Life Sciences Biotechnology Chromosome Mapping Control Cultivars Disease resistance Disease Resistance - genetics Diseases and pests Gene mapping Genes, Plant Genetic aspects Genetic Markers Genomes Genotyping Goat grass Grasses Haplotypes Humans Life Sciences Methods Nucleotide sequence Original Article Phenotyping Plant Biochemistry Plant Breeding Plant Breeding/Biotechnology Plant Diseases - genetics Plant Genetics and Genomics Plant immunology Powdery mildew Powdery mildew diseases Quantitative trait loci Recombinants Triticum - genetics Triticum aestivum Wheat
title	Fine mapping of Pm58 from Aegilops tauschii conferring powdery mildew resistance
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