C3Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry
The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles...
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| Veröffentlicht in: | Monoclonal antibodies in immunodiagnosis and immunotherapy 2022-02, Vol.41 (1), p.45-49 |
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| container_title | Monoclonal antibodies in immunodiagnosis and immunotherapy |
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| creator | Saito, Masaki Harigae, Yasuhiro Li, Guanjie Asano, Teizo Tanaka, Tomohiro Suzuki, Hiroyuki Kaneko, Mika K Kato, Yukinari |
| description | The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C
3
Mab-2; rat IgG
2b
, kappa) using the Cell-Based Immunization and Screening method and showed that C
3
Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C
3
Mab-2 and its recombinant antibody (recC
3
Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry. |
| doi_str_mv | 10.1089/mab.2021.0050 |
| format | Article |
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3
Mab-2; rat IgG
2b
, kappa) using the Cell-Based Immunization and Screening method and showed that C
3
Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C
3
Mab-2 and its recombinant antibody (recC
3
Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.</description><identifier>EISSN: 2167-9436</identifier><identifier>DOI: 10.1089/mab.2021.0050</identifier><language>eng</language><publisher>Mary Ann Liebert, Inc., publishers</publisher><subject>Short Communications</subject><ispartof>Monoclonal antibodies in immunodiagnosis and immunotherapy, 2022-02, Vol.41 (1), p.45-49</ispartof><rights>2022, Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Saito, Masaki</creatorcontrib><creatorcontrib>Harigae, Yasuhiro</creatorcontrib><creatorcontrib>Li, Guanjie</creatorcontrib><creatorcontrib>Asano, Teizo</creatorcontrib><creatorcontrib>Tanaka, Tomohiro</creatorcontrib><creatorcontrib>Suzuki, Hiroyuki</creatorcontrib><creatorcontrib>Kaneko, Mika K</creatorcontrib><creatorcontrib>Kato, Yukinari</creatorcontrib><title>C3Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry</title><title>Monoclonal antibodies in immunodiagnosis and immunotherapy</title><description>The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C
3
Mab-2; rat IgG
2b
, kappa) using the Cell-Based Immunization and Screening method and showed that C
3
Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C
3
Mab-2 and its recombinant antibody (recC
3
Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.</description><subject>Short Communications</subject><issn>2167-9436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNotkM1LxDAQxYMguKx79N6jl9ZM0iapt6XourBFED2HtJ1gpU3Wfhz635u6OwwMzHvMPH6EPABNgKr8qTdVwiiDhNKM3pANAyHjPOXijuzG8YeGksCAZxtyKHhpqpg9R3sXemrj0s8jRkXxwaPSO1933pnuX6p8s0TWD9Gx7-egLJOvv7Fvx2lY7smtNd2Iu-vckq_Xl8_iLT69H47F_hT3kMopNkyitQiq4SoXSoaUlILluaqYSNMGeW0lSGO5YZkVDUplqa0QGsXqmhm-JY-Xu-fB_844Tjr8r7HrjMMQXDPB0wwkBQhWfrH2ZliMc12LFQ6TPg_tutFA9UpLB1p6paVXWvwPKkxePQ</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Saito, Masaki</creator><creator>Harigae, Yasuhiro</creator><creator>Li, Guanjie</creator><creator>Asano, Teizo</creator><creator>Tanaka, Tomohiro</creator><creator>Suzuki, Hiroyuki</creator><creator>Kaneko, Mika K</creator><creator>Kato, Yukinari</creator><general>Mary Ann Liebert, Inc., publishers</general><scope>7X8</scope></search><sort><creationdate>20220201</creationdate><title>C3Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry</title><author>Saito, Masaki ; Harigae, Yasuhiro ; Li, Guanjie ; Asano, Teizo ; Tanaka, Tomohiro ; Suzuki, Hiroyuki ; Kaneko, Mika K ; Kato, Yukinari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-m147t-a27effe18d389687202001f398b2644de3cf717af3a25f6de78f0fbe1d82cc2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Short Communications</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saito, Masaki</creatorcontrib><creatorcontrib>Harigae, Yasuhiro</creatorcontrib><creatorcontrib>Li, Guanjie</creatorcontrib><creatorcontrib>Asano, Teizo</creatorcontrib><creatorcontrib>Tanaka, Tomohiro</creatorcontrib><creatorcontrib>Suzuki, Hiroyuki</creatorcontrib><creatorcontrib>Kaneko, Mika K</creatorcontrib><creatorcontrib>Kato, Yukinari</creatorcontrib><collection>MEDLINE - Academic</collection><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saito, Masaki</au><au>Harigae, Yasuhiro</au><au>Li, Guanjie</au><au>Asano, Teizo</au><au>Tanaka, Tomohiro</au><au>Suzuki, Hiroyuki</au><au>Kaneko, Mika K</au><au>Kato, Yukinari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C3Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry</atitle><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle><date>2022-02-01</date><risdate>2022</risdate><volume>41</volume><issue>1</issue><spage>45</spage><epage>49</epage><pages>45-49</pages><eissn>2167-9436</eissn><abstract>The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C
3
Mab-2; rat IgG
2b
, kappa) using the Cell-Based Immunization and Screening method and showed that C
3
Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C
3
Mab-2 and its recombinant antibody (recC
3
Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.</abstract><pub>Mary Ann Liebert, Inc., publishers</pub><doi>10.1089/mab.2021.0050</doi><tpages>5</tpages></addata></record> |
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| identifier | EISSN: 2167-9436 |
| ispartof | Monoclonal antibodies in immunodiagnosis and immunotherapy, 2022-02, Vol.41 (1), p.45-49 |
| issn | 2167-9436 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Short Communications |
| title | C3Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry |
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