Statistical investigation of the bioprocess conditions of alkali combined twin‐screw extrusion pretreatment to enhance fractionation and enzymatic hydrolysis of bulgur bran
BACKGROUND Bulgur bran (BB) is a potential source for the production of value‐added products such as fermentable sugars and xylooligosaccharides (XOs). In this study, alkali combined twin‐screw extrusion pretreatment was performed and statistically optimized to enhance fractionation and enzymatic hy...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2022-08, Vol.102 (11), p.4770-4779 |
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| creator | Yağcı, Sibel Sutay Kocabaş, Didem Çalışkan, Rukiye Özbek, Hatice Neval |
| description | BACKGROUND
Bulgur bran (BB) is a potential source for the production of value‐added products such as fermentable sugars and xylooligosaccharides (XOs). In this study, alkali combined twin‐screw extrusion pretreatment was performed and statistically optimized to enhance fractionation and enzymatic hydrolysis of BB. The pretreatment conditions (barrel temperature, screw speed and alkali impregnation) were optimized by Box–Behnken design (BBD) to obtain the highest hemicellulose separation from BB. The obtained fractions were analyzed for the production of fermentable sugars and XOs.
RESULTS
The results revealed that twin‐screw extrusion of BB performed at 67 °C barrel temperature and 250 rpm screw speed after alkali impregnation at 0.02 g alkali g−1 biomass concentration provided 40.4% higher hemicellulose separation yield compared to the untreated BB. Alkali combined twin‐screw extrusion pretreatment increased the enzymatic hydrolysis yield of BB fourfold, whereas a 13‐fold increase was achieved after the separation of hemicellulose from pretreated BB. Xylose (X1)‐free xylobiose (X2) was the main product after xylanase hydrolysis of hemicellulose fraction. SEM images confirmed the morphological modifications in BB, which were in agreement with the enhanced fractionation performance and the higher enzymatic hydrolysis yield.
CONCLUSION
The results of this study suggested that pretreatment by alkali combined twin‐screw extrusion followed by alkali extraction could be a reliable and effective process for fractionation of BB and production of fermentable sugars and XOs. © 2022 Society of Chemical Industry. |
| doi_str_mv | 10.1002/jsfa.11837 |
| format | Article |
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Bulgur bran (BB) is a potential source for the production of value‐added products such as fermentable sugars and xylooligosaccharides (XOs). In this study, alkali combined twin‐screw extrusion pretreatment was performed and statistically optimized to enhance fractionation and enzymatic hydrolysis of BB. The pretreatment conditions (barrel temperature, screw speed and alkali impregnation) were optimized by Box–Behnken design (BBD) to obtain the highest hemicellulose separation from BB. The obtained fractions were analyzed for the production of fermentable sugars and XOs.
RESULTS
The results revealed that twin‐screw extrusion of BB performed at 67 °C barrel temperature and 250 rpm screw speed after alkali impregnation at 0.02 g alkali g−1 biomass concentration provided 40.4% higher hemicellulose separation yield compared to the untreated BB. Alkali combined twin‐screw extrusion pretreatment increased the enzymatic hydrolysis yield of BB fourfold, whereas a 13‐fold increase was achieved after the separation of hemicellulose from pretreated BB. Xylose (X1)‐free xylobiose (X2) was the main product after xylanase hydrolysis of hemicellulose fraction. SEM images confirmed the morphological modifications in BB, which were in agreement with the enhanced fractionation performance and the higher enzymatic hydrolysis yield.
CONCLUSION
The results of this study suggested that pretreatment by alkali combined twin‐screw extrusion followed by alkali extraction could be a reliable and effective process for fractionation of BB and production of fermentable sugars and XOs. © 2022 Society of Chemical Industry.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.11837</identifier><identifier>PMID: 35218014</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Box–Behnken design ; bulgur bran ; Design optimization ; enzymatic hydrolysis ; Extrusion ; Fractionation ; Hemicellulose ; Hydrolysis ; Impregnation ; Pretreatment ; Separation ; Sugar ; twin‐screw extrusion ; Xylanase ; xylooligosaccharides (XOs)</subject><ispartof>Journal of the science of food and agriculture, 2022-08, Vol.102 (11), p.4770-4779</ispartof><rights>2022 Society of Chemical Industry.</rights><rights>Copyright © 2022 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3577-66ffaf55620c277e3d3a75d8965b4f4f97fab2de9de99f58f0b950ad2cc2160e3</citedby><cites>FETCH-LOGICAL-c3577-66ffaf55620c277e3d3a75d8965b4f4f97fab2de9de99f58f0b950ad2cc2160e3</cites><orcidid>0000-0001-5985-9539 ; 0000-0002-5689-8521 ; 0000-0001-6543-4086</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.11837$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.11837$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35218014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yağcı, Sibel</creatorcontrib><creatorcontrib>Sutay Kocabaş, Didem</creatorcontrib><creatorcontrib>Çalışkan, Rukiye</creatorcontrib><creatorcontrib>Özbek, Hatice Neval</creatorcontrib><title>Statistical investigation of the bioprocess conditions of alkali combined twin‐screw extrusion pretreatment to enhance fractionation and enzymatic hydrolysis of bulgur bran</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Bulgur bran (BB) is a potential source for the production of value‐added products such as fermentable sugars and xylooligosaccharides (XOs). In this study, alkali combined twin‐screw extrusion pretreatment was performed and statistically optimized to enhance fractionation and enzymatic hydrolysis of BB. The pretreatment conditions (barrel temperature, screw speed and alkali impregnation) were optimized by Box–Behnken design (BBD) to obtain the highest hemicellulose separation from BB. The obtained fractions were analyzed for the production of fermentable sugars and XOs.
RESULTS
The results revealed that twin‐screw extrusion of BB performed at 67 °C barrel temperature and 250 rpm screw speed after alkali impregnation at 0.02 g alkali g−1 biomass concentration provided 40.4% higher hemicellulose separation yield compared to the untreated BB. Alkali combined twin‐screw extrusion pretreatment increased the enzymatic hydrolysis yield of BB fourfold, whereas a 13‐fold increase was achieved after the separation of hemicellulose from pretreated BB. Xylose (X1)‐free xylobiose (X2) was the main product after xylanase hydrolysis of hemicellulose fraction. SEM images confirmed the morphological modifications in BB, which were in agreement with the enhanced fractionation performance and the higher enzymatic hydrolysis yield.
CONCLUSION
The results of this study suggested that pretreatment by alkali combined twin‐screw extrusion followed by alkali extraction could be a reliable and effective process for fractionation of BB and production of fermentable sugars and XOs. © 2022 Society of Chemical Industry.</description><subject>Box–Behnken design</subject><subject>bulgur bran</subject><subject>Design optimization</subject><subject>enzymatic hydrolysis</subject><subject>Extrusion</subject><subject>Fractionation</subject><subject>Hemicellulose</subject><subject>Hydrolysis</subject><subject>Impregnation</subject><subject>Pretreatment</subject><subject>Separation</subject><subject>Sugar</subject><subject>twin‐screw extrusion</subject><subject>Xylanase</subject><subject>xylooligosaccharides (XOs)</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhi0EokvhwgMgS1wQUsrYWSfrY1XRAqrEoXCOHGfc9ZLYi-10Cac-Ak_CQ_EkOE3hwAHJkj3zf_rH9k_IcwYnDIC_2UWjThjblPUDsmIg6wKAwUOyyiIvBFvzI_Ikxh0ASFlVj8lRKTjbAFuvyM-rpJKNyWrVU-tuMB-vc8c76g1NW6St9fvgNcZItXednbU4i6r_onqbm0NrHXY0Haz7dfsj6oAHit9SGONssw-YAqo0oEs0eYpuq5xGaoLSs9cyTLkuK9-nIZeabqcu-H6K9m5QO_bXY6BtUO4peWRUH_HZ_X5MPp-__XT2rrj8ePH-7PSy0KWo66KqjFFGiIqD5nWNZVeqWnQbWYl2bdZG1ka1vEOZlzRiY6CVAlTHteasAiyPyavFNz_965g_pRls1Nj3yqEfY8OrspSQB0BGX_6D7vwYXL5dpiRIBkxsMvV6oXTwMQY0zT7YQYWpYdDMKTZzis1dihl-cW85tgN2f9E_sWWALcDB9jj9x6r5cHV-upj-Bh8irf8</recordid><startdate>20220830</startdate><enddate>20220830</enddate><creator>Yağcı, Sibel</creator><creator>Sutay Kocabaş, Didem</creator><creator>Çalışkan, Rukiye</creator><creator>Özbek, Hatice Neval</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5985-9539</orcidid><orcidid>https://orcid.org/0000-0002-5689-8521</orcidid><orcidid>https://orcid.org/0000-0001-6543-4086</orcidid></search><sort><creationdate>20220830</creationdate><title>Statistical investigation of the bioprocess conditions of alkali combined twin‐screw extrusion pretreatment to enhance fractionation and enzymatic hydrolysis of bulgur bran</title><author>Yağcı, Sibel ; Sutay Kocabaş, Didem ; Çalışkan, Rukiye ; Özbek, Hatice Neval</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3577-66ffaf55620c277e3d3a75d8965b4f4f97fab2de9de99f58f0b950ad2cc2160e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Box–Behnken design</topic><topic>bulgur bran</topic><topic>Design optimization</topic><topic>enzymatic hydrolysis</topic><topic>Extrusion</topic><topic>Fractionation</topic><topic>Hemicellulose</topic><topic>Hydrolysis</topic><topic>Impregnation</topic><topic>Pretreatment</topic><topic>Separation</topic><topic>Sugar</topic><topic>twin‐screw extrusion</topic><topic>Xylanase</topic><topic>xylooligosaccharides (XOs)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yağcı, Sibel</creatorcontrib><creatorcontrib>Sutay Kocabaş, Didem</creatorcontrib><creatorcontrib>Çalışkan, Rukiye</creatorcontrib><creatorcontrib>Özbek, Hatice Neval</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yağcı, Sibel</au><au>Sutay Kocabaş, Didem</au><au>Çalışkan, Rukiye</au><au>Özbek, Hatice Neval</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Statistical investigation of the bioprocess conditions of alkali combined twin‐screw extrusion pretreatment to enhance fractionation and enzymatic hydrolysis of bulgur bran</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2022-08-30</date><risdate>2022</risdate><volume>102</volume><issue>11</issue><spage>4770</spage><epage>4779</epage><pages>4770-4779</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Bulgur bran (BB) is a potential source for the production of value‐added products such as fermentable sugars and xylooligosaccharides (XOs). In this study, alkali combined twin‐screw extrusion pretreatment was performed and statistically optimized to enhance fractionation and enzymatic hydrolysis of BB. The pretreatment conditions (barrel temperature, screw speed and alkali impregnation) were optimized by Box–Behnken design (BBD) to obtain the highest hemicellulose separation from BB. The obtained fractions were analyzed for the production of fermentable sugars and XOs.
RESULTS
The results revealed that twin‐screw extrusion of BB performed at 67 °C barrel temperature and 250 rpm screw speed after alkali impregnation at 0.02 g alkali g−1 biomass concentration provided 40.4% higher hemicellulose separation yield compared to the untreated BB. Alkali combined twin‐screw extrusion pretreatment increased the enzymatic hydrolysis yield of BB fourfold, whereas a 13‐fold increase was achieved after the separation of hemicellulose from pretreated BB. Xylose (X1)‐free xylobiose (X2) was the main product after xylanase hydrolysis of hemicellulose fraction. SEM images confirmed the morphological modifications in BB, which were in agreement with the enhanced fractionation performance and the higher enzymatic hydrolysis yield.
CONCLUSION
The results of this study suggested that pretreatment by alkali combined twin‐screw extrusion followed by alkali extraction could be a reliable and effective process for fractionation of BB and production of fermentable sugars and XOs. © 2022 Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>35218014</pmid><doi>10.1002/jsfa.11837</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5985-9539</orcidid><orcidid>https://orcid.org/0000-0002-5689-8521</orcidid><orcidid>https://orcid.org/0000-0001-6543-4086</orcidid></addata></record> |
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| ispartof | Journal of the science of food and agriculture, 2022-08, Vol.102 (11), p.4770-4779 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Box–Behnken design bulgur bran Design optimization enzymatic hydrolysis Extrusion Fractionation Hemicellulose Hydrolysis Impregnation Pretreatment Separation Sugar twin‐screw extrusion Xylanase xylooligosaccharides (XOs) |
| title | Statistical investigation of the bioprocess conditions of alkali combined twin‐screw extrusion pretreatment to enhance fractionation and enzymatic hydrolysis of bulgur bran |
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