Structure-switching aptamer triggering signal amplification strategy for tobramycin detection based on hybridization chain reaction and fluorescence synergism

Based on hybridization chain reaction (HCR) and fluorescence synergism, a novel aptasensor for tobramycin was successfully constructed. Tobramycin competed with cDNA-FAM to bind aptamers immobilized on magnetic beads. After magnetic separation, the released cDNA-FAM acted as initiator to trigger HCR...

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Veröffentlicht in:	Talanta (Oxford) 2022-06, Vol.243, p.123318-123318, Article 123318
Hauptverfasser:	Wang, Junyang, Li, Hongxia, Du, Caiyi, Li, Ying, Ma, Xinyue, Yang, Chuanyu, Xu, Wentao, Sun, Chunyan
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamers, Nucleotide - genetics Biosensing Techniques DNA - genetics Fluorescence synergism Hybridization chain reaction Limit of Detection Nucleic Acid Hybridization Signal amplification Spectrometry, Fluorescence Structure-switching aptamer Tobramycin
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