Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line
Recently, the development and application of fungal exopolysaccharides (EPS) as natural biopolymers are on the rise. The present study is based on the investigation of possible antiproliferative and antioxidant activities of EPS from the Rhodotorula mucilaginosa sp. GUMS16 on BCR-ABL positive cells...
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| Veröffentlicht in: | International journal of biological macromolecules 2022-05, Vol.206, p.21-28 |
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| creator | Kheyrandish, Setare Rastgar, Amirhossein Hamidi, Masoud Sajjadi, Seyed Mehdi Sarab, Gholamreza Anani |
| description | Recently, the development and application of fungal exopolysaccharides (EPS) as natural biopolymers are on the rise. The present study is based on the investigation of possible antiproliferative and antioxidant activities of EPS from the Rhodotorula mucilaginosa sp. GUMS16 on BCR-ABL positive cells (K562). The cytotoxicity, colony formation assays lactate and dehydrogenase (LDH) activity were performed to assess the possible cancer cell death. To elucidate the underlying antiproliferative mechanism of the EPS, cell cycle analysis following real-time PCR (gene expression assessment) were evaluated. The results indicated that, the EPS with an IC50 dose of 1500 μg/ml, reduced the viability of K562 cells without having toxic effects on normal cells as well as decrease in size and number of colonies in EPS-treated group (p |
| doi_str_mv | 10.1016/j.ijbiomac.2022.02.113 |
| format | Article |
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Graphical illustration of the developmental process of exopolysaccharide (EPS) growth inhibitory effect is provided. The EPS was exposed to K562 cells and MTT analysis so as to assess cell viability, beside RT-qPCR for investigating apoptotic and anti-apoptotic gene expressions were accomplished. In the next, colony formation assay and flow cytometry analysis were done to determine whether or not the EPS had anti-colonogenic and cell death effects, respectively. [Display omitted]
•Exopolysaccharide from GUMS16 strain was proved to be a K562 growth inhibitor.•EPS increased the expression of apoptotic genes in K562 cells.•The expression of anti-apoptotic genes was downregulated in EPS treated K562 cells.•The anti-oxidant and anti-colonogenic properties of EPS from GUMS16 strain was introduced.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2022.02.113</identifier><identifier>PMID: 35217074</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antioxidant ; Antioxidants - metabolism ; Antiproliferative ; Apoptosis ; Exopolysaccharides ; Humans ; Hydroxyurea ; K562 Cells ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics ; Rhodotorula - genetics ; Rhodotorula - metabolism ; Rhodotorula mucilaginosa</subject><ispartof>International journal of biological macromolecules, 2022-05, Vol.206, p.21-28</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-48c00bcc88f29582d8ef1dc8a0eb30eee2a500c0c44f9705a1a3cfd4f2a6e7403</citedby><cites>FETCH-LOGICAL-c368t-48c00bcc88f29582d8ef1dc8a0eb30eee2a500c0c44f9705a1a3cfd4f2a6e7403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2022.02.113$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35217074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kheyrandish, Setare</creatorcontrib><creatorcontrib>Rastgar, Amirhossein</creatorcontrib><creatorcontrib>Hamidi, Masoud</creatorcontrib><creatorcontrib>Sajjadi, Seyed Mehdi</creatorcontrib><creatorcontrib>Sarab, Gholamreza Anani</creatorcontrib><title>Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Recently, the development and application of fungal exopolysaccharides (EPS) as natural biopolymers are on the rise. The present study is based on the investigation of possible antiproliferative and antioxidant activities of EPS from the Rhodotorula mucilaginosa sp. GUMS16 on BCR-ABL positive cells (K562). The cytotoxicity, colony formation assays lactate and dehydrogenase (LDH) activity were performed to assess the possible cancer cell death. To elucidate the underlying antiproliferative mechanism of the EPS, cell cycle analysis following real-time PCR (gene expression assessment) were evaluated. The results indicated that, the EPS with an IC50 dose of 1500 μg/ml, reduced the viability of K562 cells without having toxic effects on normal cells as well as decrease in size and number of colonies in EPS-treated group (p < 0.0001). The increase of LDH was 2.75 times more than the control (p < 0.0001). Gene expression revealed up- and down-regulation of apoptotic and anti-apoptotic genes in EPS group compared with the control. Moreover, the DPPH scavenging activity of the EPS in treated cells was significantly higher than the control group (p < 0.0001). Taken together, we concluded that the EPS from GUMS16 strain is able to inhibit the growth of K562 cells besides having antioxidant activities.
Graphical illustration of the developmental process of exopolysaccharide (EPS) growth inhibitory effect is provided. The EPS was exposed to K562 cells and MTT analysis so as to assess cell viability, beside RT-qPCR for investigating apoptotic and anti-apoptotic gene expressions were accomplished. In the next, colony formation assay and flow cytometry analysis were done to determine whether or not the EPS had anti-colonogenic and cell death effects, respectively. [Display omitted]
•Exopolysaccharide from GUMS16 strain was proved to be a K562 growth inhibitor.•EPS increased the expression of apoptotic genes in K562 cells.•The expression of anti-apoptotic genes was downregulated in EPS treated K562 cells.•The anti-oxidant and anti-colonogenic properties of EPS from GUMS16 strain was introduced.</description><subject>Antioxidant</subject><subject>Antioxidants - metabolism</subject><subject>Antiproliferative</subject><subject>Apoptosis</subject><subject>Exopolysaccharides</subject><subject>Humans</subject><subject>Hydroxyurea</subject><subject>K562 Cells</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics</subject><subject>Rhodotorula - genetics</subject><subject>Rhodotorula - metabolism</subject><subject>Rhodotorula mucilaginosa</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uFDEQhC0EIkvgFSIfOTBD2_PnvYGiEBBBSEDOVq_dZr14xovtCez78KDMahOunFoqVXV162PsQkAtQPSvd7XfbXwc0dQSpKxB1kI0j9hKqGFdAUDzmK1AtKJSooEz9izn3aL2nVBP2VnTSTHA0K7Yn6s7DDMWHyceHcep-KrMY0ycnCNTjmLZEqffcR_DIaMxW0zeEncpjnyiX9zEYCu0uC9k-YEwl1f8yzbaWGKaA_JxNj7gdz_FjDzva359--mr6PnSaLYpTt7w8UAhessDzT9o9Mg_dr3khkLgwU_0nD1xGDK9uJ_n7Pbd1bfL99XN5-sPl29vKtP0qlStMgAbY5Ryct0paRU5YY1CoE0DRCSxAzBg2tatB-hQYGOcbZ3EnoYWmnP28rR3n-LPmXLRo8_HK3CiOGct-6ZRXddBt1j7k9WkmHMip_fJj5gOWoA-EtI7_UBIHwlpkHohtAQv7jvmzUj2X-wByWJ4czLQ8umdp6Sz8TQZsj4tQLSN_n8dfwE5Y6fw</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Kheyrandish, Setare</creator><creator>Rastgar, Amirhossein</creator><creator>Hamidi, Masoud</creator><creator>Sajjadi, Seyed Mehdi</creator><creator>Sarab, Gholamreza Anani</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220501</creationdate><title>Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line</title><author>Kheyrandish, Setare ; Rastgar, Amirhossein ; Hamidi, Masoud ; Sajjadi, Seyed Mehdi ; Sarab, Gholamreza Anani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-48c00bcc88f29582d8ef1dc8a0eb30eee2a500c0c44f9705a1a3cfd4f2a6e7403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antioxidant</topic><topic>Antioxidants - metabolism</topic><topic>Antiproliferative</topic><topic>Apoptosis</topic><topic>Exopolysaccharides</topic><topic>Humans</topic><topic>Hydroxyurea</topic><topic>K562 Cells</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics</topic><topic>Rhodotorula - genetics</topic><topic>Rhodotorula - metabolism</topic><topic>Rhodotorula mucilaginosa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kheyrandish, Setare</creatorcontrib><creatorcontrib>Rastgar, Amirhossein</creatorcontrib><creatorcontrib>Hamidi, Masoud</creatorcontrib><creatorcontrib>Sajjadi, Seyed Mehdi</creatorcontrib><creatorcontrib>Sarab, Gholamreza Anani</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kheyrandish, Setare</au><au>Rastgar, Amirhossein</au><au>Hamidi, Masoud</au><au>Sajjadi, Seyed Mehdi</au><au>Sarab, Gholamreza Anani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>206</volume><spage>21</spage><epage>28</epage><pages>21-28</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Recently, the development and application of fungal exopolysaccharides (EPS) as natural biopolymers are on the rise. The present study is based on the investigation of possible antiproliferative and antioxidant activities of EPS from the Rhodotorula mucilaginosa sp. GUMS16 on BCR-ABL positive cells (K562). The cytotoxicity, colony formation assays lactate and dehydrogenase (LDH) activity were performed to assess the possible cancer cell death. To elucidate the underlying antiproliferative mechanism of the EPS, cell cycle analysis following real-time PCR (gene expression assessment) were evaluated. The results indicated that, the EPS with an IC50 dose of 1500 μg/ml, reduced the viability of K562 cells without having toxic effects on normal cells as well as decrease in size and number of colonies in EPS-treated group (p < 0.0001). The increase of LDH was 2.75 times more than the control (p < 0.0001). Gene expression revealed up- and down-regulation of apoptotic and anti-apoptotic genes in EPS group compared with the control. Moreover, the DPPH scavenging activity of the EPS in treated cells was significantly higher than the control group (p < 0.0001). Taken together, we concluded that the EPS from GUMS16 strain is able to inhibit the growth of K562 cells besides having antioxidant activities.
Graphical illustration of the developmental process of exopolysaccharide (EPS) growth inhibitory effect is provided. The EPS was exposed to K562 cells and MTT analysis so as to assess cell viability, beside RT-qPCR for investigating apoptotic and anti-apoptotic gene expressions were accomplished. In the next, colony formation assay and flow cytometry analysis were done to determine whether or not the EPS had anti-colonogenic and cell death effects, respectively. [Display omitted]
•Exopolysaccharide from GUMS16 strain was proved to be a K562 growth inhibitor.•EPS increased the expression of apoptotic genes in K562 cells.•The expression of anti-apoptotic genes was downregulated in EPS treated K562 cells.•The anti-oxidant and anti-colonogenic properties of EPS from GUMS16 strain was introduced.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>35217074</pmid><doi>10.1016/j.ijbiomac.2022.02.113</doi><tpages>8</tpages></addata></record> |
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| ispartof | International journal of biological macromolecules, 2022-05, Vol.206, p.21-28 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antioxidant Antioxidants - metabolism Antiproliferative Apoptosis Exopolysaccharides Humans Hydroxyurea K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Rhodotorula - genetics Rhodotorula - metabolism Rhodotorula mucilaginosa |
| title | Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line |
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