Prolonged exposure of human spermatozoa in polyvinylpyrrolidone has detrimental effects on sperm biological characteristics
Polyvinylpyrrolidone (PVP) has been utilized in intracytoplasmic sperm injection (ICSI) for immobilization and manipulation of spermatozoa. This study aims to determine the suitable time that sperm cells could be safely exposed to PVP during ICSI procedure. Twenty‐five normal semen samples were prep...
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| Veröffentlicht in: | Andrologia 2022-07, Vol.54 (6), p.e14402-n/a |
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| creator | Sabour, Mojdeh Agha‐Rahimi, Azam Dehghani Ashkezari, Mahmood Seifati, Seyed Morteza Anbari, Fatemeh Nabi, Ali |
| description | Polyvinylpyrrolidone (PVP) has been utilized in intracytoplasmic sperm injection (ICSI) for immobilization and manipulation of spermatozoa. This study aims to determine the suitable time that sperm cells could be safely exposed to PVP during ICSI procedure. Twenty‐five normal semen samples were prepared using the swim‐up method and then were exposed to 10% PVP at different time intervals (15, 30 and 60 min). The effect of PVP on sperm parameters (viability and morphology), DNA fragmentation index (sperm chromatin dispersion test), chromatin quality (aniline blue, toluidine blue and chromomycin A3 staining), acrosome reaction, mitochondrial membrane potential and sperm ultrastructure was assessed at different time intervals. Our results showed that prolonged sperm exposure in PVP for 15, 30 and 60 min significantly affects viability and morphology with a concomitant increase in DNA fragmentation and abnormal chromatin structure, while the percentage of acrosome‐reacted spermatozoa was additionally increased. In addition, the spermatozoa with high mitochondrial membrane potential were significantly decreased compared to unexposed spermatozoa to PVP. In conclusion, the detrimental effects of PVP were increased significantly following sperm exposure in PVP after 15 min. Therefore, the sperm exposure to PVP should be limited to less than 15 min during ICSI procedure. |
| doi_str_mv | 10.1111/and.14402 |
| format | Article |
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This study aims to determine the suitable time that sperm cells could be safely exposed to PVP during ICSI procedure. Twenty‐five normal semen samples were prepared using the swim‐up method and then were exposed to 10% PVP at different time intervals (15, 30 and 60 min). The effect of PVP on sperm parameters (viability and morphology), DNA fragmentation index (sperm chromatin dispersion test), chromatin quality (aniline blue, toluidine blue and chromomycin A3 staining), acrosome reaction, mitochondrial membrane potential and sperm ultrastructure was assessed at different time intervals. Our results showed that prolonged sperm exposure in PVP for 15, 30 and 60 min significantly affects viability and morphology with a concomitant increase in DNA fragmentation and abnormal chromatin structure, while the percentage of acrosome‐reacted spermatozoa was additionally increased. In addition, the spermatozoa with high mitochondrial membrane potential were significantly decreased compared to unexposed spermatozoa to PVP. In conclusion, the detrimental effects of PVP were increased significantly following sperm exposure in PVP after 15 min. Therefore, the sperm exposure to PVP should be limited to less than 15 min during ICSI procedure.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/and.14402</identifier><identifier>PMID: 35212016</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Acrosome reaction ; Aniline ; Chromatin ; chromatin structure ; Deoxyribonucleic acid ; DNA ; DNA fragmentation ; Immobilization ; Membrane potential ; Mitochondria ; mitochondrial membrane potential ; Morphology ; Polyvinylpyrrolidone ; Sperm ; sperm DNA fragmentation ; sperm parameters ; Toluidine ; Ultrastructure</subject><ispartof>Andrologia, 2022-07, Vol.54 (6), p.e14402-n/a</ispartof><rights>2022 Wiley‐VCH GmbH</rights><rights>2022 Wiley-VCH GmbH.</rights><rights>Copyright © 2022 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-bdbd9a78c00fbc41e91a77a07225fe93d06e7167cd1cfd1f416213b6b0996b0e3</citedby><cites>FETCH-LOGICAL-c3532-bdbd9a78c00fbc41e91a77a07225fe93d06e7167cd1cfd1f416213b6b0996b0e3</cites><orcidid>0000-0002-7579-6192 ; 0000-0002-3794-6073 ; 0000-0001-6967-2305</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fand.14402$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fand.14402$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35212016$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabour, Mojdeh</creatorcontrib><creatorcontrib>Agha‐Rahimi, Azam</creatorcontrib><creatorcontrib>Dehghani Ashkezari, Mahmood</creatorcontrib><creatorcontrib>Seifati, Seyed Morteza</creatorcontrib><creatorcontrib>Anbari, Fatemeh</creatorcontrib><creatorcontrib>Nabi, Ali</creatorcontrib><title>Prolonged exposure of human spermatozoa in polyvinylpyrrolidone has detrimental effects on sperm biological characteristics</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Polyvinylpyrrolidone (PVP) has been utilized in intracytoplasmic sperm injection (ICSI) for immobilization and manipulation of spermatozoa. This study aims to determine the suitable time that sperm cells could be safely exposed to PVP during ICSI procedure. Twenty‐five normal semen samples were prepared using the swim‐up method and then were exposed to 10% PVP at different time intervals (15, 30 and 60 min). The effect of PVP on sperm parameters (viability and morphology), DNA fragmentation index (sperm chromatin dispersion test), chromatin quality (aniline blue, toluidine blue and chromomycin A3 staining), acrosome reaction, mitochondrial membrane potential and sperm ultrastructure was assessed at different time intervals. Our results showed that prolonged sperm exposure in PVP for 15, 30 and 60 min significantly affects viability and morphology with a concomitant increase in DNA fragmentation and abnormal chromatin structure, while the percentage of acrosome‐reacted spermatozoa was additionally increased. In addition, the spermatozoa with high mitochondrial membrane potential were significantly decreased compared to unexposed spermatozoa to PVP. In conclusion, the detrimental effects of PVP were increased significantly following sperm exposure in PVP after 15 min. Therefore, the sperm exposure to PVP should be limited to less than 15 min during ICSI procedure.</description><subject>Acrosome reaction</subject><subject>Aniline</subject><subject>Chromatin</subject><subject>chromatin structure</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fragmentation</subject><subject>Immobilization</subject><subject>Membrane potential</subject><subject>Mitochondria</subject><subject>mitochondrial membrane potential</subject><subject>Morphology</subject><subject>Polyvinylpyrrolidone</subject><subject>Sperm</subject><subject>sperm DNA fragmentation</subject><subject>sperm parameters</subject><subject>Toluidine</subject><subject>Ultrastructure</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kU1v1DAQhi0Eoqu2B_4AssQFDmn97c2xKhSQqrYHOEeOPe66SuxgJ0DKn8ewCwck5jBzmGfemdGL0AtKzmiNcxPdGRWCsCdoQwVvG8I0e4o2hBPeCKnaI3RaygOpIaTWQjxHR1wyyghVG_TjLqchxXtwGL5PqSwZcPJ4t4wm4jJBHs2cHpPBIeIpDevXENdhWnOdCi5FwDtTsIM5hxHibAYM3oOdC06HcdyHuuA-2NqzO5ONnSGHMgdbTtAzb4YCp4d6jD5fvft0-aG5vn3_8fLiurFcctb0rnet0VtLiO-toNBSo7UhmjHpoeWOKNBUaeuo9Y56QRWjvFc9aduagB-j13vdKacvC5S5G0OxMAwmQlpKxxTnW0larir66h_0IS051usqpRVlUm5lpd7sKZtTKRl8N9X_TV47SrpfpnTVlO63KZV9eVBc-hHcX_KPBRU43wPfwgDr_5W6i5u3e8mf7tyYPA</recordid><startdate>202207</startdate><enddate>202207</enddate><creator>Sabour, Mojdeh</creator><creator>Agha‐Rahimi, Azam</creator><creator>Dehghani Ashkezari, Mahmood</creator><creator>Seifati, Seyed Morteza</creator><creator>Anbari, Fatemeh</creator><creator>Nabi, Ali</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7579-6192</orcidid><orcidid>https://orcid.org/0000-0002-3794-6073</orcidid><orcidid>https://orcid.org/0000-0001-6967-2305</orcidid></search><sort><creationdate>202207</creationdate><title>Prolonged exposure of human spermatozoa in polyvinylpyrrolidone has detrimental effects on sperm biological characteristics</title><author>Sabour, Mojdeh ; Agha‐Rahimi, Azam ; Dehghani Ashkezari, Mahmood ; Seifati, Seyed Morteza ; Anbari, Fatemeh ; Nabi, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-bdbd9a78c00fbc41e91a77a07225fe93d06e7167cd1cfd1f416213b6b0996b0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acrosome reaction</topic><topic>Aniline</topic><topic>Chromatin</topic><topic>chromatin structure</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fragmentation</topic><topic>Immobilization</topic><topic>Membrane potential</topic><topic>Mitochondria</topic><topic>mitochondrial membrane potential</topic><topic>Morphology</topic><topic>Polyvinylpyrrolidone</topic><topic>Sperm</topic><topic>sperm DNA fragmentation</topic><topic>sperm parameters</topic><topic>Toluidine</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabour, Mojdeh</creatorcontrib><creatorcontrib>Agha‐Rahimi, Azam</creatorcontrib><creatorcontrib>Dehghani Ashkezari, Mahmood</creatorcontrib><creatorcontrib>Seifati, Seyed Morteza</creatorcontrib><creatorcontrib>Anbari, Fatemeh</creatorcontrib><creatorcontrib>Nabi, Ali</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabour, Mojdeh</au><au>Agha‐Rahimi, Azam</au><au>Dehghani Ashkezari, Mahmood</au><au>Seifati, Seyed Morteza</au><au>Anbari, Fatemeh</au><au>Nabi, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prolonged exposure of human spermatozoa in polyvinylpyrrolidone has detrimental effects on sperm biological characteristics</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2022-07</date><risdate>2022</risdate><volume>54</volume><issue>6</issue><spage>e14402</spage><epage>n/a</epage><pages>e14402-n/a</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Polyvinylpyrrolidone (PVP) has been utilized in intracytoplasmic sperm injection (ICSI) for immobilization and manipulation of spermatozoa. This study aims to determine the suitable time that sperm cells could be safely exposed to PVP during ICSI procedure. Twenty‐five normal semen samples were prepared using the swim‐up method and then were exposed to 10% PVP at different time intervals (15, 30 and 60 min). The effect of PVP on sperm parameters (viability and morphology), DNA fragmentation index (sperm chromatin dispersion test), chromatin quality (aniline blue, toluidine blue and chromomycin A3 staining), acrosome reaction, mitochondrial membrane potential and sperm ultrastructure was assessed at different time intervals. Our results showed that prolonged sperm exposure in PVP for 15, 30 and 60 min significantly affects viability and morphology with a concomitant increase in DNA fragmentation and abnormal chromatin structure, while the percentage of acrosome‐reacted spermatozoa was additionally increased. In addition, the spermatozoa with high mitochondrial membrane potential were significantly decreased compared to unexposed spermatozoa to PVP. In conclusion, the detrimental effects of PVP were increased significantly following sperm exposure in PVP after 15 min. Therefore, the sperm exposure to PVP should be limited to less than 15 min during ICSI procedure.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35212016</pmid><doi>10.1111/and.14402</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7579-6192</orcidid><orcidid>https://orcid.org/0000-0002-3794-6073</orcidid><orcidid>https://orcid.org/0000-0001-6967-2305</orcidid></addata></record> |
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| subjects | Acrosome reaction Aniline Chromatin chromatin structure Deoxyribonucleic acid DNA DNA fragmentation Immobilization Membrane potential Mitochondria mitochondrial membrane potential Morphology Polyvinylpyrrolidone Sperm sperm DNA fragmentation sperm parameters Toluidine Ultrastructure |
| title | Prolonged exposure of human spermatozoa in polyvinylpyrrolidone has detrimental effects on sperm biological characteristics |
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