Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae)
BACKGROUND RNA‐mediated interference (RNAi) has become a promising biopesticide technology with which to direct sequence‐specific gene knockdown of key targets in the potato psyllid (PoP) Bactericera cockerelli, resulting in significant mortality. In this study, three strategically selected target g...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2022-06, Vol.78 (6), p.2204-2214 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2214 |
|---|---|
| container_issue | 6 |
| container_start_page | 2204 |
| container_title | Pest management science |
| container_volume | 78 |
| creator | Paredes‐Montero, Jorge R Arif, Usman Brown, Judith K |
| description | BACKGROUND
RNA‐mediated interference (RNAi) has become a promising biopesticide technology with which to direct sequence‐specific gene knockdown of key targets in the potato psyllid (PoP) Bactericera cockerelli, resulting in significant mortality. In this study, three strategically selected target genes, ATF4, C7 and D24, essential for the biosynthesis and regulation of ecdysteroids, were evaluated for knockdown and mortality using oral delivery of individual, paired and all three double‐stranded RNAs (dsRNAs), in five replicated experiments. Knockdown was determined as the fold‐change in gene expression using a quantitative polymerase chain reaction.
RESULTS
Knockdown of the D24 target, at 39%–45%, resulted in 51% PoP mortality by 10 days post‐ingestion (dpi) of dsRNA. Knockdown of C7, at 38%–61%, resulted in 53% mortality by 10 dpi, whereas dsD24 ingestion resulted in 65% mortality by 10 dpi when dsD24 and dsC7 were co‐delivered. Three phenotypes, INCOMEC, PREMEC and SWOLLEN, were observed at a frequency of 4%–12%, and are consistent with incomplete ecdysis in immature and/or adult PoP. Adult PoP exhibiting INCOMEC survived for several days but were unable to mate or fly, whereas SWOLLEN and PREMEC were lethal to the immature instars. Knockdown of ATF4 did not result in the mortality or malformations in immature and adult PoP.
CONCLUSIONS
Compared with knockdown of individual D24 and C7 targets, significantly greater RNAi penetrance was achieved following delivery of combined dsRNAs. The highest knockdown that resulted in incomplete ecdysis and/or mortality was obtained for targets with predicted involvement in the same or interacting pathway(s). Knockdown of ATF4 was apparently “rescued” by uncharacterized compensatory gene(s) or effects. © 2022 Society of Chemical Industry.
RNA interference‐mediated knockdown of genes implicated in the synthesis of ecdysteroids from phytosterols resulted in impaired molting and high mortality in the potato psyllid, Bactericera cockerelli. |
| doi_str_mv | 10.1002/ps.6848 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2631864335</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2631864335</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3788-da79e360f58328de677bd30b37a966d0dab58f9b439802f490c0e4a0747a258f3</originalsourceid><addsrcrecordid>eNp10VFr1TAUB_AgiptT_AYS8MENuTNp2jTxTcd0w4GCE3wraXJ6b2ab1JyWUT_BPvZyvdseBJ8Scn78c-BPyEvOjjljxbsRj6Uq1SOyz6tCrkqt1eOHu_q5R54hXjHGtNbFU7InKq4512yf3HwJ0f5y8TrQ2FGwbsEJUvSO4hKmDaBHuoYASBPg3E9IfaB-GI1P4OgQ-8mHNTXB0Y1fb_JDmkzvp2XLPhqbs7yFZKjNv0CCvvf08AwGP-aJeU8vk49_vDNw9Jw86UyP8OLuPCA_Pp1enpytLr5-Pj_5cLGyolZq5UytQUjWVUoUyoGs69YJ1oraaCkdc6atVKfbUmjFiq7UzDIoDavL2hR5Ig7I4S53TPH3DDg1g0ebFzMB4oxNIQVXshSiyvT1P_Qqzink7bKSlSy04jqrNztlU0RM0DVj8oNJS8NZsy2nGbHZlpPlq7u8uR3APbj7NjJ4uwPXvoflfznNt-9_424BGNKY7g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2665629819</pqid></control><display><type>article</type><title>Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae)</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Paredes‐Montero, Jorge R ; Arif, Usman ; Brown, Judith K</creator><creatorcontrib>Paredes‐Montero, Jorge R ; Arif, Usman ; Brown, Judith K</creatorcontrib><description>BACKGROUND
RNA‐mediated interference (RNAi) has become a promising biopesticide technology with which to direct sequence‐specific gene knockdown of key targets in the potato psyllid (PoP) Bactericera cockerelli, resulting in significant mortality. In this study, three strategically selected target genes, ATF4, C7 and D24, essential for the biosynthesis and regulation of ecdysteroids, were evaluated for knockdown and mortality using oral delivery of individual, paired and all three double‐stranded RNAs (dsRNAs), in five replicated experiments. Knockdown was determined as the fold‐change in gene expression using a quantitative polymerase chain reaction.
RESULTS
Knockdown of the D24 target, at 39%–45%, resulted in 51% PoP mortality by 10 days post‐ingestion (dpi) of dsRNA. Knockdown of C7, at 38%–61%, resulted in 53% mortality by 10 dpi, whereas dsD24 ingestion resulted in 65% mortality by 10 dpi when dsD24 and dsC7 were co‐delivered. Three phenotypes, INCOMEC, PREMEC and SWOLLEN, were observed at a frequency of 4%–12%, and are consistent with incomplete ecdysis in immature and/or adult PoP. Adult PoP exhibiting INCOMEC survived for several days but were unable to mate or fly, whereas SWOLLEN and PREMEC were lethal to the immature instars. Knockdown of ATF4 did not result in the mortality or malformations in immature and adult PoP.
CONCLUSIONS
Compared with knockdown of individual D24 and C7 targets, significantly greater RNAi penetrance was achieved following delivery of combined dsRNAs. The highest knockdown that resulted in incomplete ecdysis and/or mortality was obtained for targets with predicted involvement in the same or interacting pathway(s). Knockdown of ATF4 was apparently “rescued” by uncharacterized compensatory gene(s) or effects. © 2022 Society of Chemical Industry.
RNA interference‐mediated knockdown of genes implicated in the synthesis of ecdysteroids from phytosterols resulted in impaired molting and high mortality in the potato psyllid, Bactericera cockerelli.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.6848</identifier><identifier>PMID: 35191190</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Asian citrus psyllid ; Bactericera cockerelli ; biopesticide ; Biosynthesis ; Double-stranded RNA ; Ecdysteroids ; Gene expression ; Genes ; Hemiptera - genetics ; Ingestion ; Instars ; Molting ; Molting - genetics ; Mortality ; Pesticides ; Phenotypes ; Polymerase chain reaction ; potato psyllid ; reverse genetics ; RNA, Double-Stranded ; RNA-mediated interference ; Solanum tuberosum - genetics</subject><ispartof>Pest management science, 2022-06, Vol.78 (6), p.2204-2214</ispartof><rights>2022 Society of Chemical Industry.</rights><rights>Copyright © 2022 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3788-da79e360f58328de677bd30b37a966d0dab58f9b439802f490c0e4a0747a258f3</citedby><cites>FETCH-LOGICAL-c3788-da79e360f58328de677bd30b37a966d0dab58f9b439802f490c0e4a0747a258f3</cites><orcidid>0000-0002-5495-2379 ; 0000-0003-4669-8443</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.6848$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.6848$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35191190$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paredes‐Montero, Jorge R</creatorcontrib><creatorcontrib>Arif, Usman</creatorcontrib><creatorcontrib>Brown, Judith K</creatorcontrib><title>Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae)</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
RNA‐mediated interference (RNAi) has become a promising biopesticide technology with which to direct sequence‐specific gene knockdown of key targets in the potato psyllid (PoP) Bactericera cockerelli, resulting in significant mortality. In this study, three strategically selected target genes, ATF4, C7 and D24, essential for the biosynthesis and regulation of ecdysteroids, were evaluated for knockdown and mortality using oral delivery of individual, paired and all three double‐stranded RNAs (dsRNAs), in five replicated experiments. Knockdown was determined as the fold‐change in gene expression using a quantitative polymerase chain reaction.
RESULTS
Knockdown of the D24 target, at 39%–45%, resulted in 51% PoP mortality by 10 days post‐ingestion (dpi) of dsRNA. Knockdown of C7, at 38%–61%, resulted in 53% mortality by 10 dpi, whereas dsD24 ingestion resulted in 65% mortality by 10 dpi when dsD24 and dsC7 were co‐delivered. Three phenotypes, INCOMEC, PREMEC and SWOLLEN, were observed at a frequency of 4%–12%, and are consistent with incomplete ecdysis in immature and/or adult PoP. Adult PoP exhibiting INCOMEC survived for several days but were unable to mate or fly, whereas SWOLLEN and PREMEC were lethal to the immature instars. Knockdown of ATF4 did not result in the mortality or malformations in immature and adult PoP.
CONCLUSIONS
Compared with knockdown of individual D24 and C7 targets, significantly greater RNAi penetrance was achieved following delivery of combined dsRNAs. The highest knockdown that resulted in incomplete ecdysis and/or mortality was obtained for targets with predicted involvement in the same or interacting pathway(s). Knockdown of ATF4 was apparently “rescued” by uncharacterized compensatory gene(s) or effects. © 2022 Society of Chemical Industry.
RNA interference‐mediated knockdown of genes implicated in the synthesis of ecdysteroids from phytosterols resulted in impaired molting and high mortality in the potato psyllid, Bactericera cockerelli.</description><subject>Animals</subject><subject>Asian citrus psyllid</subject><subject>Bactericera cockerelli</subject><subject>biopesticide</subject><subject>Biosynthesis</subject><subject>Double-stranded RNA</subject><subject>Ecdysteroids</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Hemiptera - genetics</subject><subject>Ingestion</subject><subject>Instars</subject><subject>Molting</subject><subject>Molting - genetics</subject><subject>Mortality</subject><subject>Pesticides</subject><subject>Phenotypes</subject><subject>Polymerase chain reaction</subject><subject>potato psyllid</subject><subject>reverse genetics</subject><subject>RNA, Double-Stranded</subject><subject>RNA-mediated interference</subject><subject>Solanum tuberosum - genetics</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10VFr1TAUB_AgiptT_AYS8MENuTNp2jTxTcd0w4GCE3wraXJ6b2ab1JyWUT_BPvZyvdseBJ8Scn78c-BPyEvOjjljxbsRj6Uq1SOyz6tCrkqt1eOHu_q5R54hXjHGtNbFU7InKq4512yf3HwJ0f5y8TrQ2FGwbsEJUvSO4hKmDaBHuoYASBPg3E9IfaB-GI1P4OgQ-8mHNTXB0Y1fb_JDmkzvp2XLPhqbs7yFZKjNv0CCvvf08AwGP-aJeU8vk49_vDNw9Jw86UyP8OLuPCA_Pp1enpytLr5-Pj_5cLGyolZq5UytQUjWVUoUyoGs69YJ1oraaCkdc6atVKfbUmjFiq7UzDIoDavL2hR5Ig7I4S53TPH3DDg1g0ebFzMB4oxNIQVXshSiyvT1P_Qqzink7bKSlSy04jqrNztlU0RM0DVj8oNJS8NZsy2nGbHZlpPlq7u8uR3APbj7NjJ4uwPXvoflfznNt-9_424BGNKY7g</recordid><startdate>202206</startdate><enddate>202206</enddate><creator>Paredes‐Montero, Jorge R</creator><creator>Arif, Usman</creator><creator>Brown, Judith K</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5495-2379</orcidid><orcidid>https://orcid.org/0000-0003-4669-8443</orcidid></search><sort><creationdate>202206</creationdate><title>Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae)</title><author>Paredes‐Montero, Jorge R ; Arif, Usman ; Brown, Judith K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3788-da79e360f58328de677bd30b37a966d0dab58f9b439802f490c0e4a0747a258f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Asian citrus psyllid</topic><topic>Bactericera cockerelli</topic><topic>biopesticide</topic><topic>Biosynthesis</topic><topic>Double-stranded RNA</topic><topic>Ecdysteroids</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hemiptera - genetics</topic><topic>Ingestion</topic><topic>Instars</topic><topic>Molting</topic><topic>Molting - genetics</topic><topic>Mortality</topic><topic>Pesticides</topic><topic>Phenotypes</topic><topic>Polymerase chain reaction</topic><topic>potato psyllid</topic><topic>reverse genetics</topic><topic>RNA, Double-Stranded</topic><topic>RNA-mediated interference</topic><topic>Solanum tuberosum - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paredes‐Montero, Jorge R</creatorcontrib><creatorcontrib>Arif, Usman</creatorcontrib><creatorcontrib>Brown, Judith K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paredes‐Montero, Jorge R</au><au>Arif, Usman</au><au>Brown, Judith K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae)</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2022-06</date><risdate>2022</risdate><volume>78</volume><issue>6</issue><spage>2204</spage><epage>2214</epage><pages>2204-2214</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
RNA‐mediated interference (RNAi) has become a promising biopesticide technology with which to direct sequence‐specific gene knockdown of key targets in the potato psyllid (PoP) Bactericera cockerelli, resulting in significant mortality. In this study, three strategically selected target genes, ATF4, C7 and D24, essential for the biosynthesis and regulation of ecdysteroids, were evaluated for knockdown and mortality using oral delivery of individual, paired and all three double‐stranded RNAs (dsRNAs), in five replicated experiments. Knockdown was determined as the fold‐change in gene expression using a quantitative polymerase chain reaction.
RESULTS
Knockdown of the D24 target, at 39%–45%, resulted in 51% PoP mortality by 10 days post‐ingestion (dpi) of dsRNA. Knockdown of C7, at 38%–61%, resulted in 53% mortality by 10 dpi, whereas dsD24 ingestion resulted in 65% mortality by 10 dpi when dsD24 and dsC7 were co‐delivered. Three phenotypes, INCOMEC, PREMEC and SWOLLEN, were observed at a frequency of 4%–12%, and are consistent with incomplete ecdysis in immature and/or adult PoP. Adult PoP exhibiting INCOMEC survived for several days but were unable to mate or fly, whereas SWOLLEN and PREMEC were lethal to the immature instars. Knockdown of ATF4 did not result in the mortality or malformations in immature and adult PoP.
CONCLUSIONS
Compared with knockdown of individual D24 and C7 targets, significantly greater RNAi penetrance was achieved following delivery of combined dsRNAs. The highest knockdown that resulted in incomplete ecdysis and/or mortality was obtained for targets with predicted involvement in the same or interacting pathway(s). Knockdown of ATF4 was apparently “rescued” by uncharacterized compensatory gene(s) or effects. © 2022 Society of Chemical Industry.
RNA interference‐mediated knockdown of genes implicated in the synthesis of ecdysteroids from phytosterols resulted in impaired molting and high mortality in the potato psyllid, Bactericera cockerelli.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>35191190</pmid><doi>10.1002/ps.6848</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-5495-2379</orcidid><orcidid>https://orcid.org/0000-0003-4669-8443</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2022-06, Vol.78 (6), p.2204-2214 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2631864335 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animals Asian citrus psyllid Bactericera cockerelli biopesticide Biosynthesis Double-stranded RNA Ecdysteroids Gene expression Genes Hemiptera - genetics Ingestion Instars Molting Molting - genetics Mortality Pesticides Phenotypes Polymerase chain reaction potato psyllid reverse genetics RNA, Double-Stranded RNA-mediated interference Solanum tuberosum - genetics |
| title | Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T01%3A33%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Knockdown%20of%20ecdysteroid%20synthesis%20genes%20results%20in%20impaired%20molting%20and%20high%20mortality%20in%20Bactericera%20cockerelli%20(Hemiptera:%20Triozidae)&rft.jtitle=Pest%20management%20science&rft.au=Paredes%E2%80%90Montero,%20Jorge%20R&rft.date=2022-06&rft.volume=78&rft.issue=6&rft.spage=2204&rft.epage=2214&rft.pages=2204-2214&rft.issn=1526-498X&rft.eissn=1526-4998&rft_id=info:doi/10.1002/ps.6848&rft_dat=%3Cproquest_cross%3E2631864335%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2665629819&rft_id=info:pmid/35191190&rfr_iscdi=true |