High-resolution structure of phosphoketolase from Bifidobacterium longum determined by cryo-EM single-particle analysis

[Display omitted] •3D structure of Bifidobacterium longum phosphoketolase was determined at 2.1 Å resolution by the cryo-EM single particle analysis.•Structural difference between cryo-EM and XRD structures: almost identical except for a small loop (QN-loop) near the substrate binding site.•QN-loop...

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Veröffentlicht in:	Journal of structural biology 2022-06, Vol.214 (2), p.107842-107842, Article 107842
Hauptverfasser:	Nakata, Kunio, Miyazaki, Naoyuki, Yamaguchi, Hiroki, Hirose, Mika, Kashiwagi, Tatsuki, Kutumbarao, Nidamarthi H.V., Miyashita, Osamu, Tama, Florence, Miyano, Hiroshi, Mizukoshi, Toshimi, Iwasaki, Kenji
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde-Lyases - chemistry Atomic resolution Bifidobacterium longum - enzymology Conformational change Cryo-EM Cryoelectron Microscopy - methods Escherichia coli Models, Molecular Multi-conformer Phosphoketolase Single-particle analysis Thiamine Pyrophosphate Water
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