Combined bioreaction and separation in centrifugal fields using zonal rotors
Combined biosynthesis and product separation has been successfully performed for the first time using a zonal centrifugal bioreactor–separator. The biosynthesis of dextran polymer from sucrose using the dextransucrase enzyme was investigated in order to evaluate bioreactor performance. The bioreacto...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 1994-09, Vol.61 (1), p.19-29 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 61 |
| creator | Setford, Steven J. Barker, Phillip E. |
| description | Combined biosynthesis and product separation has been successfully performed for the first time using a zonal centrifugal bioreactor–separator. The biosynthesis of dextran polymer from sucrose using the dextransucrase enzyme was investigated in order to evaluate bioreactor performance. The bioreactor consisted of a bowl, fitted with a Reorienting gradient rotor which facilitated substrate and enzyme loading and the reproducible unloading of solutions from the system. This allowed the distribution of substrate, enzyme and product materials in the bowl at the end of each trial to be accurately determined. Studies have indicated that combined bioreaction–separation is possible using this system. However, viscosity build‐up in the bioreactor must be minimised in order to increase the yield of polymer product per unit time and improve product separation. |
| doi_str_mv | 10.1002/jctb.280610104 |
| format | Article |
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The biosynthesis of dextran polymer from sucrose using the dextransucrase enzyme was investigated in order to evaluate bioreactor performance. The bioreactor consisted of a bowl, fitted with a Reorienting gradient rotor which facilitated substrate and enzyme loading and the reproducible unloading of solutions from the system. This allowed the distribution of substrate, enzyme and product materials in the bowl at the end of each trial to be accurately determined. Studies have indicated that combined bioreaction–separation is possible using this system. However, viscosity build‐up in the bioreactor must be minimised in order to increase the yield of polymer product per unit time and improve product separation.</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.280610104</identifier><identifier>CODEN: JCTBDC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Biological and medical sciences ; bioreactor ; Biotechnology ; centrifugation ; dextran ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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Chem. Technol. Biotechnol</addtitle><description>Combined biosynthesis and product separation has been successfully performed for the first time using a zonal centrifugal bioreactor–separator. The biosynthesis of dextran polymer from sucrose using the dextransucrase enzyme was investigated in order to evaluate bioreactor performance. The bioreactor consisted of a bowl, fitted with a Reorienting gradient rotor which facilitated substrate and enzyme loading and the reproducible unloading of solutions from the system. This allowed the distribution of substrate, enzyme and product materials in the bowl at the end of each trial to be accurately determined. Studies have indicated that combined bioreaction–separation is possible using this system. However, viscosity build‐up in the bioreactor must be minimised in order to increase the yield of polymer product per unit time and improve product separation.</description><subject>Biological and medical sciences</subject><subject>bioreactor</subject><subject>Biotechnology</subject><subject>centrifugation</subject><subject>dextran</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Others</subject><subject>reactor-separator</subject><subject>separation</subject><subject>Various methods and equipments</subject><subject>zonal</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNo9kM1LAzEQxYMoWKtXz3sQb1snySbNHqVoFauC37eQzUdJ3SY12YL619va0tMwM7_3eDyETjEMMAC5mOmuGRABHAOGag_1MNTDsuIc9lEPCBclYUN2iI5yngEAF4T30GQU540P1hSNj8kq3fkYChVMke1CJfW_-lBoG7rk3XKq2sJ525pcLLMP0-I3htUpxS6mfIwOnGqzPdnOPnq9vnoZ3ZSTx_Ht6HJSakpZVzYWmDZA1TpppQ0DgS3UgmrshCa2dsbWCgO3hpIGE3DGac4qxirtBDa0j843vosUv5Y2d3Lus7Ztq4KNyywJp0Ax5StwsAF1ijkn6-Qi-blKPxKDXJcm16XJXWkrwdnWWWWtWpdU0D7vVJTUosZihZUbzOfOfu_eKn1KPqRDJt8fxrJ6I0_3188f8o7-AepufO4</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Setford, Steven J.</creator><creator>Barker, Phillip E.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>19940901</creationdate><title>Combined bioreaction and separation in centrifugal fields using zonal rotors</title><author>Setford, Steven J. ; Barker, Phillip E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-be05cd03a61014cd5081e0983c1f8c2e9fde9a106ed32b120fdfc654554cf81d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>bioreactor</topic><topic>Biotechnology</topic><topic>centrifugation</topic><topic>dextran</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Others</topic><topic>reactor-separator</topic><topic>separation</topic><topic>Various methods and equipments</topic><topic>zonal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Setford, Steven J.</creatorcontrib><creatorcontrib>Barker, Phillip E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Setford, Steven J.</au><au>Barker, Phillip E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined bioreaction and separation in centrifugal fields using zonal rotors</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><addtitle>J. Chem. Technol. Biotechnol</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>61</volume><issue>1</issue><spage>19</spage><epage>29</epage><pages>19-29</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><coden>JCTBDC</coden><abstract>Combined biosynthesis and product separation has been successfully performed for the first time using a zonal centrifugal bioreactor–separator. The biosynthesis of dextran polymer from sucrose using the dextransucrase enzyme was investigated in order to evaluate bioreactor performance. The bioreactor consisted of a bowl, fitted with a Reorienting gradient rotor which facilitated substrate and enzyme loading and the reproducible unloading of solutions from the system. This allowed the distribution of substrate, enzyme and product materials in the bowl at the end of each trial to be accurately determined. Studies have indicated that combined bioreaction–separation is possible using this system. However, viscosity build‐up in the bioreactor must be minimised in order to increase the yield of polymer product per unit time and improve product separation.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.280610104</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-2575 |
| ispartof | Journal of chemical technology and biotechnology (1986), 1994-09, Vol.61 (1), p.19-29 |
| issn | 0268-2575 1097-4660 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_26303136 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences bioreactor Biotechnology centrifugation dextran Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others reactor-separator separation Various methods and equipments zonal |
| title | Combined bioreaction and separation in centrifugal fields using zonal rotors |
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