Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants
The emergence of vancomycin-resistant Enterococcus faecium (Efm) harboring vanA gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment p...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2022-04, Vol.28 (4), p.444-452 |
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| container_title | Microbial drug resistance (Larchmont, N.Y.) |
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| creator | Farias, Beatriz Oliveira de Bianco, Kayo Nascimento, Ana Paula Alves Gonçalves de Brito, Andressa Silva Moreira, Thais Costa Clementino, Maysa Mandetta |
| description | The emergence of vancomycin-resistant
Enterococcus faecium
(Efm) harboring
vanA
gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of
vanA
-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight
vanA
-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as
aph(3′)-IIIa
,
ant(6′)-Ia
,
erm(B)
, and
msrC
, some of them being allocated in plasmids. The virulence profile was predominantly constituted by
efaAfm
and
acm
genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of
vanA
-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs. |
| doi_str_mv | 10.1089/mdr.2021.0254 |
| format | Article |
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Enterococcus faecium
(Efm) harboring
vanA
gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of
vanA
-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight
vanA
-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as
aph(3′)-IIIa
,
ant(6′)-Ia
,
erm(B)
, and
msrC
, some of them being allocated in plasmids. The virulence profile was predominantly constituted by
efaAfm
and
acm
genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of
vanA
-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2021.0254</identifier><identifier>PMID: 35172112</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Antibiotic resistance ; Antibiotics ; Antimicrobial resistance ; Drug resistance ; Enterococcus faecium ; Epidemiology ; Gene sequencing ; Genes ; Genomic analysis ; Genomics ; Multidrug resistance ; Multidrug resistant organisms ; Multilocus sequence typing ; Nosocomial infection ; Opportunist infection ; Pathogens ; Plasmids ; Public health ; Sewage ; Sewage disposal ; VanA gene ; Vancomycin ; Virulence ; Wastewater treatment ; Wastewater treatment plants ; Water treatment ; Whole genome sequencing</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2022-04, Vol.28 (4), p.444-452</ispartof><rights>2022, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Apr 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-88c2f45e3b96a51ee29cf8eaec5346be7282b995f21640aaff76270a142652ff3</citedby><cites>FETCH-LOGICAL-c404t-88c2f45e3b96a51ee29cf8eaec5346be7282b995f21640aaff76270a142652ff3</cites><orcidid>0000-0001-6320-712X ; 0000-0002-7520-4097</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35172112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farias, Beatriz Oliveira de</creatorcontrib><creatorcontrib>Bianco, Kayo</creatorcontrib><creatorcontrib>Nascimento, Ana Paula Alves</creatorcontrib><creatorcontrib>Gonçalves de Brito, Andressa Silva</creatorcontrib><creatorcontrib>Moreira, Thais Costa</creatorcontrib><creatorcontrib>Clementino, Maysa Mandetta</creatorcontrib><title>Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>The emergence of vancomycin-resistant
Enterococcus faecium
(Efm) harboring
vanA
gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of
vanA
-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight
vanA
-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as
aph(3′)-IIIa
,
ant(6′)-Ia
,
erm(B)
, and
msrC
, some of them being allocated in plasmids. The virulence profile was predominantly constituted by
efaAfm
and
acm
genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of
vanA
-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs.</description><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Drug resistance</subject><subject>Enterococcus faecium</subject><subject>Epidemiology</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomic analysis</subject><subject>Genomics</subject><subject>Multidrug resistance</subject><subject>Multidrug resistant organisms</subject><subject>Multilocus sequence typing</subject><subject>Nosocomial infection</subject><subject>Opportunist infection</subject><subject>Pathogens</subject><subject>Plasmids</subject><subject>Public health</subject><subject>Sewage</subject><subject>Sewage disposal</subject><subject>VanA gene</subject><subject>Vancomycin</subject><subject>Virulence</subject><subject>Wastewater treatment</subject><subject>Wastewater treatment plants</subject><subject>Water treatment</subject><subject>Whole genome sequencing</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkUtLxDAURoMoPkaXbiXgxk3HvNsuh2EcBUURxWVJMzdSaRsnSRX_vSkzunDjKuFycnL5PoROKZlSUpSX3cpPGWF0SpgUO-iQlpxmhRDFbrqTXGWKleIAHYXwRgiRVPF9dMAlzRml7BCtl9C7rjF41uv2KzQBO4vvhjY2Kz-8Zo-QRlH3ES_6CN4ZZ8wQsNVgmqHD19rXzjf9K_7Q_QwnF2DrXYdfdIjwqdMT_ORBxw6S4qFNonCM9qxuA5xszwl6vlo8za-z2_vlzXx2mxlBRMyKwjArJPC6VFpSAFYaW0D6WHKhashZweqylJZRJYjW1uaK5URTwZRk1vIJuth4371bDxBi1TXBQJuWADeEiqVgCkWkIgk9_4O-ucGnQEZKcpbnVPBEZRvKeBeCB1u9-6bT_quipBq7qFIX1dhFNXaR-LOtdag7WP3SP-EngG-Acaz7vm2gBh__0X4Dz5SWog</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Farias, Beatriz Oliveira de</creator><creator>Bianco, Kayo</creator><creator>Nascimento, Ana Paula Alves</creator><creator>Gonçalves de Brito, Andressa Silva</creator><creator>Moreira, Thais Costa</creator><creator>Clementino, Maysa Mandetta</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6320-712X</orcidid><orcidid>https://orcid.org/0000-0002-7520-4097</orcidid></search><sort><creationdate>20220401</creationdate><title>Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants</title><author>Farias, Beatriz Oliveira de ; Bianco, Kayo ; Nascimento, Ana Paula Alves ; Gonçalves de Brito, Andressa Silva ; Moreira, Thais Costa ; Clementino, Maysa Mandetta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-88c2f45e3b96a51ee29cf8eaec5346be7282b995f21640aaff76270a142652ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Drug resistance</topic><topic>Enterococcus faecium</topic><topic>Epidemiology</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genomic analysis</topic><topic>Genomics</topic><topic>Multidrug resistance</topic><topic>Multidrug resistant organisms</topic><topic>Multilocus sequence typing</topic><topic>Nosocomial infection</topic><topic>Opportunist infection</topic><topic>Pathogens</topic><topic>Plasmids</topic><topic>Public health</topic><topic>Sewage</topic><topic>Sewage disposal</topic><topic>VanA gene</topic><topic>Vancomycin</topic><topic>Virulence</topic><topic>Wastewater treatment</topic><topic>Wastewater treatment plants</topic><topic>Water treatment</topic><topic>Whole genome sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farias, Beatriz Oliveira de</creatorcontrib><creatorcontrib>Bianco, Kayo</creatorcontrib><creatorcontrib>Nascimento, Ana Paula Alves</creatorcontrib><creatorcontrib>Gonçalves de Brito, Andressa Silva</creatorcontrib><creatorcontrib>Moreira, Thais Costa</creatorcontrib><creatorcontrib>Clementino, Maysa Mandetta</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farias, Beatriz Oliveira de</au><au>Bianco, Kayo</au><au>Nascimento, Ana Paula Alves</au><au>Gonçalves de Brito, Andressa Silva</au><au>Moreira, Thais Costa</au><au>Clementino, Maysa Mandetta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2022-04-01</date><risdate>2022</risdate><volume>28</volume><issue>4</issue><spage>444</spage><epage>452</epage><pages>444-452</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>The emergence of vancomycin-resistant
Enterococcus faecium
(Efm) harboring
vanA
gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of
vanA
-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight
vanA
-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as
aph(3′)-IIIa
,
ant(6′)-Ia
,
erm(B)
, and
msrC
, some of them being allocated in plasmids. The virulence profile was predominantly constituted by
efaAfm
and
acm
genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of
vanA
-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>35172112</pmid><doi>10.1089/mdr.2021.0254</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6320-712X</orcidid><orcidid>https://orcid.org/0000-0002-7520-4097</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2022-04, Vol.28 (4), p.444-452 |
| issn | 1076-6294 1931-8448 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Antibiotic resistance Antibiotics Antimicrobial resistance Drug resistance Enterococcus faecium Epidemiology Gene sequencing Genes Genomic analysis Genomics Multidrug resistance Multidrug resistant organisms Multilocus sequence typing Nosocomial infection Opportunist infection Pathogens Plasmids Public health Sewage Sewage disposal VanA gene Vancomycin Virulence Wastewater treatment Wastewater treatment plants Water treatment Whole genome sequencing |
| title | Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants |
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