Structure‐based engineering of a short‐chain cis‐prenyltransferase to biosynthesize nonnatural all‐cis‐polyisoprenoids: molecular mechanisms for primer substrate recognition and ultimate product chain‐length determination

Most cis‐prenyltransferases (cPTs) use all‐trans‐oligoprenyl diphosphate, such as (E,E)‐farnesyl diphosphate (FPP, C15), but scarcely accept dimethylallyl diphosphate (DMAPP, C5), as an allylic diphosphate primer in consecutive cis‐condensations of isopentenyl diphosphate. Consequently, naturally oc...

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Veröffentlicht in:	The FEBS journal 2022-08, Vol.289 (15), p.4602-4621
Hauptverfasser:	Kutsukawa, Ryo, Imaizumi, Riki, Suenaga‐Hiromori, Miki, Takeshita, Kohei, Sakai, Naoki, Misawa, Shuto, Yamamoto, Masaki, Yamaguchi, Haruhiko, Miyagi‐Inoue, Yukino, Waki, Toshiyuki, Kataoka, Kunishige, Nakayama, Toru, Yamashita, Satoshi, Takahashi, Seiji
Format:	Artikel
Sprache:	eng
Schlagworte:	Chains cis‐prenyltransferase Crystal structure Diphosphates Domains Enzymatic synthesis Hydrophobicity Isopentenyl diphosphate Isoprene isoprenoid Molecular modelling Mutants neryl diphosphate synthase Prenyltransferases Solanaceae Solanum lycopersicum Substrate preferences Substrates Tomatoes
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