Variability in the composition of porcine mucosal heparan sulfates
Commercial porcine intestinal mucosal heparan sulfate (HS) is a valuable material for research into its biological functions. As it is usually produced as a side-stream of pharmaceutical heparin manufacture, its chemical composition may vary from batch to batch. We analysed the composition and struc...
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| Veröffentlicht in: | Carbohydrate polymers 2022-04, Vol.282, p.119081-119081, Article 119081 |
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| creator | Sargison, Liam Smith, Raymond A.A. Carnachan, Susan M. Daines, Alison M. Brackovic, Amira Kidgell, Joel T. Nurcombe, Victor Cool, Simon M. Sims, Ian M. Hinkley, Simon F.R. |
| description | Commercial porcine intestinal mucosal heparan sulfate (HS) is a valuable material for research into its biological functions. As it is usually produced as a side-stream of pharmaceutical heparin manufacture, its chemical composition may vary from batch to batch. We analysed the composition and structure of nine batches of HS from the same manufacturer. Statistical analysis of the disaccharide compositions placed these batches in three categories: group A had high GlcNAc and GlcNS, and low GlcN typical of HS; group B had high GlcN and GlcNS, and low GlcNAc; group C had high di- and trisulfated, and low unsulfated and monosulfated disaccharide repeats. These batches could be placed in the same categories based on their 1H NMR spectra and molecular weights. Anticoagulant and growth factor binding activities of these HS batches did not fit within these same groups but were related to the proportions of more highly sulfated disaccharide repeats.
[Display omitted]
•The composition and structure of commercial batches of heparan sulfates were determined by HPLC, SEC and NMR.•Batches of commercial heparan sulfate have different anticoagulant and growth factor binding activity.•Our research highlights the need to determine the composition and structure of HS to understand its biological activity |
| doi_str_mv | 10.1016/j.carbpol.2021.119081 |
| format | Article |
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[Display omitted]
•The composition and structure of commercial batches of heparan sulfates were determined by HPLC, SEC and NMR.•Batches of commercial heparan sulfate have different anticoagulant and growth factor binding activity.•Our research highlights the need to determine the composition and structure of HS to understand its biological activity</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2021.119081</identifier><identifier>PMID: 35123736</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Anticoagulant activity ; Anticoagulants - chemistry ; Disaccharide analysis ; Disaccharides - analysis ; Factor Xa - chemistry ; Growth factor binding ; Heparan sulfate ; Heparitin Sulfate - chemistry ; Intercellular Signaling Peptides and Proteins - chemistry ; Intestinal Mucosa - chemistry ; Nuclear magnetic resonance spectroscopy ; Size-exclusion chromatography ; Swine</subject><ispartof>Carbohydrate polymers, 2022-04, Vol.282, p.119081-119081, Article 119081</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-5f6b4b22ff3c305e9aa4cf07cbcd4d49d8bb363853063ff0de3ea39445f8f4e73</citedby><cites>FETCH-LOGICAL-c365t-5f6b4b22ff3c305e9aa4cf07cbcd4d49d8bb363853063ff0de3ea39445f8f4e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861721014685$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35123736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sargison, Liam</creatorcontrib><creatorcontrib>Smith, Raymond A.A.</creatorcontrib><creatorcontrib>Carnachan, Susan M.</creatorcontrib><creatorcontrib>Daines, Alison M.</creatorcontrib><creatorcontrib>Brackovic, Amira</creatorcontrib><creatorcontrib>Kidgell, Joel T.</creatorcontrib><creatorcontrib>Nurcombe, Victor</creatorcontrib><creatorcontrib>Cool, Simon M.</creatorcontrib><creatorcontrib>Sims, Ian M.</creatorcontrib><creatorcontrib>Hinkley, Simon F.R.</creatorcontrib><title>Variability in the composition of porcine mucosal heparan sulfates</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>Commercial porcine intestinal mucosal heparan sulfate (HS) is a valuable material for research into its biological functions. As it is usually produced as a side-stream of pharmaceutical heparin manufacture, its chemical composition may vary from batch to batch. We analysed the composition and structure of nine batches of HS from the same manufacturer. Statistical analysis of the disaccharide compositions placed these batches in three categories: group A had high GlcNAc and GlcNS, and low GlcN typical of HS; group B had high GlcN and GlcNS, and low GlcNAc; group C had high di- and trisulfated, and low unsulfated and monosulfated disaccharide repeats. These batches could be placed in the same categories based on their 1H NMR spectra and molecular weights. Anticoagulant and growth factor binding activities of these HS batches did not fit within these same groups but were related to the proportions of more highly sulfated disaccharide repeats.
[Display omitted]
•The composition and structure of commercial batches of heparan sulfates were determined by HPLC, SEC and NMR.•Batches of commercial heparan sulfate have different anticoagulant and growth factor binding activity.•Our research highlights the need to determine the composition and structure of HS to understand its biological activity</description><subject>Animals</subject><subject>Anticoagulant activity</subject><subject>Anticoagulants - chemistry</subject><subject>Disaccharide analysis</subject><subject>Disaccharides - analysis</subject><subject>Factor Xa - chemistry</subject><subject>Growth factor binding</subject><subject>Heparan sulfate</subject><subject>Heparitin Sulfate - chemistry</subject><subject>Intercellular Signaling Peptides and Proteins - chemistry</subject><subject>Intestinal Mucosa - chemistry</subject><subject>Nuclear magnetic resonance spectroscopy</subject><subject>Size-exclusion chromatography</subject><subject>Swine</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EglL4CSCPLAl27DjJhKDiS6rEAqyW7ZxVV0kc7ASp_55UKazcDbc8753uQeiKkpQSKm63qVFB975JM5LRlNKKlPQILWhZVAllnB-jBaGcJ6WgxRk6j3FLphKUnKIzltOMFUws0MOnCk5p17hhh12Hhw1g49veRzc432Fvce-DcR3gdjQ-qgZvoFdBdTiOjVUDxAt0YlUT4fIwl-jj6fF99ZKs355fV_frxDCRD0luheY6y6xlhpEcKqW4saQw2tS85lVdas0EK3NGBLOW1MBAsYrz3JaWQ8GW6Gbe2wf_NUIcZOuigaZRHfgxykxMnXFR0gnNZ9QEH2MAK_vgWhV2khK51ye38qBP7vXJWd-Uuz6cGHUL9V_q19cE3M0ATI9-OwgyGgedgdoFMIOsvfvnxA_B8oQQ</recordid><startdate>20220415</startdate><enddate>20220415</enddate><creator>Sargison, Liam</creator><creator>Smith, Raymond A.A.</creator><creator>Carnachan, Susan M.</creator><creator>Daines, Alison M.</creator><creator>Brackovic, Amira</creator><creator>Kidgell, Joel T.</creator><creator>Nurcombe, Victor</creator><creator>Cool, Simon M.</creator><creator>Sims, Ian M.</creator><creator>Hinkley, Simon F.R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220415</creationdate><title>Variability in the composition of porcine mucosal heparan sulfates</title><author>Sargison, Liam ; Smith, Raymond A.A. ; Carnachan, Susan M. ; Daines, Alison M. ; Brackovic, Amira ; Kidgell, Joel T. ; Nurcombe, Victor ; Cool, Simon M. ; Sims, Ian M. ; Hinkley, Simon F.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-5f6b4b22ff3c305e9aa4cf07cbcd4d49d8bb363853063ff0de3ea39445f8f4e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Anticoagulant activity</topic><topic>Anticoagulants - chemistry</topic><topic>Disaccharide analysis</topic><topic>Disaccharides - analysis</topic><topic>Factor Xa - chemistry</topic><topic>Growth factor binding</topic><topic>Heparan sulfate</topic><topic>Heparitin Sulfate - chemistry</topic><topic>Intercellular Signaling Peptides and Proteins - chemistry</topic><topic>Intestinal Mucosa - chemistry</topic><topic>Nuclear magnetic resonance spectroscopy</topic><topic>Size-exclusion chromatography</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sargison, Liam</creatorcontrib><creatorcontrib>Smith, Raymond A.A.</creatorcontrib><creatorcontrib>Carnachan, Susan M.</creatorcontrib><creatorcontrib>Daines, Alison M.</creatorcontrib><creatorcontrib>Brackovic, Amira</creatorcontrib><creatorcontrib>Kidgell, Joel T.</creatorcontrib><creatorcontrib>Nurcombe, Victor</creatorcontrib><creatorcontrib>Cool, Simon M.</creatorcontrib><creatorcontrib>Sims, Ian M.</creatorcontrib><creatorcontrib>Hinkley, Simon F.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sargison, Liam</au><au>Smith, Raymond A.A.</au><au>Carnachan, Susan M.</au><au>Daines, Alison M.</au><au>Brackovic, Amira</au><au>Kidgell, Joel T.</au><au>Nurcombe, Victor</au><au>Cool, Simon M.</au><au>Sims, Ian M.</au><au>Hinkley, Simon F.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variability in the composition of porcine mucosal heparan sulfates</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2022-04-15</date><risdate>2022</risdate><volume>282</volume><spage>119081</spage><epage>119081</epage><pages>119081-119081</pages><artnum>119081</artnum><issn>0144-8617</issn><eissn>1879-1344</eissn><abstract>Commercial porcine intestinal mucosal heparan sulfate (HS) is a valuable material for research into its biological functions. As it is usually produced as a side-stream of pharmaceutical heparin manufacture, its chemical composition may vary from batch to batch. We analysed the composition and structure of nine batches of HS from the same manufacturer. Statistical analysis of the disaccharide compositions placed these batches in three categories: group A had high GlcNAc and GlcNS, and low GlcN typical of HS; group B had high GlcN and GlcNS, and low GlcNAc; group C had high di- and trisulfated, and low unsulfated and monosulfated disaccharide repeats. These batches could be placed in the same categories based on their 1H NMR spectra and molecular weights. Anticoagulant and growth factor binding activities of these HS batches did not fit within these same groups but were related to the proportions of more highly sulfated disaccharide repeats.
[Display omitted]
•The composition and structure of commercial batches of heparan sulfates were determined by HPLC, SEC and NMR.•Batches of commercial heparan sulfate have different anticoagulant and growth factor binding activity.•Our research highlights the need to determine the composition and structure of HS to understand its biological activity</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>35123736</pmid><doi>10.1016/j.carbpol.2021.119081</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Anticoagulant activity Anticoagulants - chemistry Disaccharide analysis Disaccharides - analysis Factor Xa - chemistry Growth factor binding Heparan sulfate Heparitin Sulfate - chemistry Intercellular Signaling Peptides and Proteins - chemistry Intestinal Mucosa - chemistry Nuclear magnetic resonance spectroscopy Size-exclusion chromatography Swine |
| title | Variability in the composition of porcine mucosal heparan sulfates |
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