Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms
Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms ( E...
Gespeichert in:
| Veröffentlicht in: | Environmental science and pollution research international 2022-06, Vol.29 (26), p.40012-40028 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 40028 |
|---|---|
| container_issue | 26 |
| container_start_page | 40012 |
| container_title | Environmental science and pollution research international |
| container_volume | 29 |
| creator | He, Falin Yu, Hanmei Shi, Huijian Li, Xiangxiang Chu, Shanshan Huo, Chengqian Liu, Rutao |
| description | Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (
Eisenia fetida
) in natural soil matrix. PHE exhibited a relatively high toxicity to
E. fetida
in natural soil, with the LC
50
determined to be 56.68 mg kg
−1
after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in
E. fetida
, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in
E. fetida
responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5 mg kg
−1
) and habitat function loss (10 mg kg
−1
). Histological observations indicated that the intestine, body wall, and seminal vesicle in
E. fetida
were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward
E. fetida
at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirmed that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in
E. fetida
. These effects can trigger behavior changes and damage histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the eco-toxicity potential of exogenous environmental pollutants in a real soil environment. |
| doi_str_mv | 10.1007/s11356-022-18990-w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2625268448</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2625268448</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-dca85bed9046e2c7d7c6f52cf76105e15958d174013668109f8b9dec03a22da13</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi0EokPhBVggS2xY1OBbnGQJVblIldjAOvLYJxNXiT34OB14Hx4UT6eAxIKVJZ_v_32sj5Dngr8WnLdvUAjVGMalZKLre84OD8hGGKFZq_v-IdnwXmsmlNZn5AniDeeS97J9TM5UU6OqUxvy8x1M9jakbOcLOgUsaW_LlOa0C-54tYMIJbgLaqOnKe9sDLiwQ_BAM-A-RQSkJdH9BNHGMuXKsxD96qDy34O3JdwCxVJppCHSq4AQg6VjrfWWgs1lOqS83A2jLWvdhGIKM12Cy8klXPApeTTaGeHZ_XlOvr6_-nL5kV1__vDp8u01c6ptCvPOds0WfM-1Aela3zozNtKNrRG8AdH0TedFq7lQxnSC92O37T04rqyU3gp1Tl6devc5fVsBy7AEdDDPNkJacZBGNtJ0WncVffkPepPWHOt2lTKmFYKbY6E8UfUniBnGYZ_DYvOPQfDh6HA4ORyqw-HO4XCooRf31et2Af8n8ltaBdQJwDqKO8h_3_5P7S8mnquE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2666711061</pqid></control><display><type>article</type><title>Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>He, Falin ; Yu, Hanmei ; Shi, Huijian ; Li, Xiangxiang ; Chu, Shanshan ; Huo, Chengqian ; Liu, Rutao</creator><creatorcontrib>He, Falin ; Yu, Hanmei ; Shi, Huijian ; Li, Xiangxiang ; Chu, Shanshan ; Huo, Chengqian ; Liu, Rutao</creatorcontrib><description>Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (
Eisenia fetida
) in natural soil matrix. PHE exhibited a relatively high toxicity to
E. fetida
in natural soil, with the LC
50
determined to be 56.68 mg kg
−1
after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in
E. fetida
, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in
E. fetida
responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5 mg kg
−1
) and habitat function loss (10 mg kg
−1
). Histological observations indicated that the intestine, body wall, and seminal vesicle in
E. fetida
were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward
E. fetida
at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirmed that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in
E. fetida
. These effects can trigger behavior changes and damage histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the eco-toxicity potential of exogenous environmental pollutants in a real soil environment.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-022-18990-w</identifier><identifier>PMID: 35113383</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Antioxidants ; Antioxidants - metabolism ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Biomarkers ; Body wall ; Carbonyls ; Carboxylesterase ; Catalase ; Catalase - metabolism ; Contaminants ; Contamination ; Damage ; DNA damage ; Earth and Environmental Science ; Ecotoxicology ; Eisenia fetida ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Exposure ; Free radicals ; Genotoxicity ; Glutathione ; Glutathione transferase ; Intestine ; Lipid peroxidation ; Lipids ; Malondialdehyde - metabolism ; Microcosms ; Oligochaeta ; Oxidative Stress ; Peroxidase ; Peroxidation ; Phenanthrene ; Phenanthrenes - metabolism ; Pollutants ; Reactive Oxygen Species - metabolism ; Research Article ; Seminal vesicle ; Soil - chemistry ; Soil contamination ; Soil environment ; Soil Pollutants - metabolism ; Soil pollution ; Soil quality ; Soils ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Toxicity ; Waste Water Technology ; Water Management ; Water Pollution Control ; Worms</subject><ispartof>Environmental science and pollution research international, 2022-06, Vol.29 (26), p.40012-40028</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-dca85bed9046e2c7d7c6f52cf76105e15958d174013668109f8b9dec03a22da13</citedby><cites>FETCH-LOGICAL-c375t-dca85bed9046e2c7d7c6f52cf76105e15958d174013668109f8b9dec03a22da13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-022-18990-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-022-18990-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35113383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Falin</creatorcontrib><creatorcontrib>Yu, Hanmei</creatorcontrib><creatorcontrib>Shi, Huijian</creatorcontrib><creatorcontrib>Li, Xiangxiang</creatorcontrib><creatorcontrib>Chu, Shanshan</creatorcontrib><creatorcontrib>Huo, Chengqian</creatorcontrib><creatorcontrib>Liu, Rutao</creatorcontrib><title>Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (
Eisenia fetida
) in natural soil matrix. PHE exhibited a relatively high toxicity to
E. fetida
in natural soil, with the LC
50
determined to be 56.68 mg kg
−1
after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in
E. fetida
, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in
E. fetida
responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5 mg kg
−1
) and habitat function loss (10 mg kg
−1
). Histological observations indicated that the intestine, body wall, and seminal vesicle in
E. fetida
were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward
E. fetida
at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirmed that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in
E. fetida
. These effects can trigger behavior changes and damage histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the eco-toxicity potential of exogenous environmental pollutants in a real soil environment.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Biomarkers</subject><subject>Body wall</subject><subject>Carbonyls</subject><subject>Carboxylesterase</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Contaminants</subject><subject>Contamination</subject><subject>Damage</subject><subject>DNA damage</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Eisenia fetida</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Exposure</subject><subject>Free radicals</subject><subject>Genotoxicity</subject><subject>Glutathione</subject><subject>Glutathione transferase</subject><subject>Intestine</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Malondialdehyde - metabolism</subject><subject>Microcosms</subject><subject>Oligochaeta</subject><subject>Oxidative Stress</subject><subject>Peroxidase</subject><subject>Peroxidation</subject><subject>Phenanthrene</subject><subject>Phenanthrenes - metabolism</subject><subject>Pollutants</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Research Article</subject><subject>Seminal vesicle</subject><subject>Soil - chemistry</subject><subject>Soil contamination</subject><subject>Soil environment</subject><subject>Soil Pollutants - metabolism</subject><subject>Soil pollution</subject><subject>Soil quality</subject><subject>Soils</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Toxicity</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>Worms</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kctu1DAUhi0EokPhBVggS2xY1OBbnGQJVblIldjAOvLYJxNXiT34OB14Hx4UT6eAxIKVJZ_v_32sj5Dngr8WnLdvUAjVGMalZKLre84OD8hGGKFZq_v-IdnwXmsmlNZn5AniDeeS97J9TM5UU6OqUxvy8x1M9jakbOcLOgUsaW_LlOa0C-54tYMIJbgLaqOnKe9sDLiwQ_BAM-A-RQSkJdH9BNHGMuXKsxD96qDy34O3JdwCxVJppCHSq4AQg6VjrfWWgs1lOqS83A2jLWvdhGIKM12Cy8klXPApeTTaGeHZ_XlOvr6_-nL5kV1__vDp8u01c6ptCvPOds0WfM-1Aela3zozNtKNrRG8AdH0TedFq7lQxnSC92O37T04rqyU3gp1Tl6devc5fVsBy7AEdDDPNkJacZBGNtJ0WncVffkPepPWHOt2lTKmFYKbY6E8UfUniBnGYZ_DYvOPQfDh6HA4ORyqw-HO4XCooRf31et2Af8n8ltaBdQJwDqKO8h_3_5P7S8mnquE</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>He, Falin</creator><creator>Yu, Hanmei</creator><creator>Shi, Huijian</creator><creator>Li, Xiangxiang</creator><creator>Chu, Shanshan</creator><creator>Huo, Chengqian</creator><creator>Liu, Rutao</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20220601</creationdate><title>Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms</title><author>He, Falin ; Yu, Hanmei ; Shi, Huijian ; Li, Xiangxiang ; Chu, Shanshan ; Huo, Chengqian ; Liu, Rutao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-dca85bed9046e2c7d7c6f52cf76105e15958d174013668109f8b9dec03a22da13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - metabolism</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Biomarkers</topic><topic>Body wall</topic><topic>Carbonyls</topic><topic>Carboxylesterase</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Contaminants</topic><topic>Contamination</topic><topic>Damage</topic><topic>DNA damage</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Eisenia fetida</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Exposure</topic><topic>Free radicals</topic><topic>Genotoxicity</topic><topic>Glutathione</topic><topic>Glutathione transferase</topic><topic>Intestine</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Malondialdehyde - metabolism</topic><topic>Microcosms</topic><topic>Oligochaeta</topic><topic>Oxidative Stress</topic><topic>Peroxidase</topic><topic>Peroxidation</topic><topic>Phenanthrene</topic><topic>Phenanthrenes - metabolism</topic><topic>Pollutants</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Research Article</topic><topic>Seminal vesicle</topic><topic>Soil - chemistry</topic><topic>Soil contamination</topic><topic>Soil environment</topic><topic>Soil Pollutants - metabolism</topic><topic>Soil pollution</topic><topic>Soil quality</topic><topic>Soils</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Toxicity</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Falin</creatorcontrib><creatorcontrib>Yu, Hanmei</creatorcontrib><creatorcontrib>Shi, Huijian</creatorcontrib><creatorcontrib>Li, Xiangxiang</creatorcontrib><creatorcontrib>Chu, Shanshan</creatorcontrib><creatorcontrib>Huo, Chengqian</creatorcontrib><creatorcontrib>Liu, Rutao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Falin</au><au>Yu, Hanmei</au><au>Shi, Huijian</au><au>Li, Xiangxiang</au><au>Chu, Shanshan</au><au>Huo, Chengqian</au><au>Liu, Rutao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>29</volume><issue>26</issue><spage>40012</spage><epage>40028</epage><pages>40012-40028</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (
Eisenia fetida
) in natural soil matrix. PHE exhibited a relatively high toxicity to
E. fetida
in natural soil, with the LC
50
determined to be 56.68 mg kg
−1
after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in
E. fetida
, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in
E. fetida
responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5 mg kg
−1
) and habitat function loss (10 mg kg
−1
). Histological observations indicated that the intestine, body wall, and seminal vesicle in
E. fetida
were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward
E. fetida
at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirmed that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in
E. fetida
. These effects can trigger behavior changes and damage histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the eco-toxicity potential of exogenous environmental pollutants in a real soil environment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35113383</pmid><doi>10.1007/s11356-022-18990-w</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-1344 |
| ispartof | Environmental science and pollution research international, 2022-06, Vol.29 (26), p.40012-40028 |
| issn | 0944-1344 1614-7499 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2625268448 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Antioxidants Antioxidants - metabolism Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Biomarkers Body wall Carbonyls Carboxylesterase Catalase Catalase - metabolism Contaminants Contamination Damage DNA damage Earth and Environmental Science Ecotoxicology Eisenia fetida Environment Environmental Chemistry Environmental Health Environmental science Exposure Free radicals Genotoxicity Glutathione Glutathione transferase Intestine Lipid peroxidation Lipids Malondialdehyde - metabolism Microcosms Oligochaeta Oxidative Stress Peroxidase Peroxidation Phenanthrene Phenanthrenes - metabolism Pollutants Reactive Oxygen Species - metabolism Research Article Seminal vesicle Soil - chemistry Soil contamination Soil environment Soil Pollutants - metabolism Soil pollution Soil quality Soils Superoxide dismutase Superoxide Dismutase - metabolism Toxicity Waste Water Technology Water Management Water Pollution Control Worms |
| title | Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T22%3A09%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Behavioral,%20histopathological,%20genetic,%20and%20organism-wide%20responses%20to%20phenanthrene-induced%20oxidative%20stress%20in%20Eisenia%20fetida%20earthworms%20in%20natural%20soil%20microcosms&rft.jtitle=Environmental%20science%20and%20pollution%20research%20international&rft.au=He,%20Falin&rft.date=2022-06-01&rft.volume=29&rft.issue=26&rft.spage=40012&rft.epage=40028&rft.pages=40012-40028&rft.issn=0944-1344&rft.eissn=1614-7499&rft_id=info:doi/10.1007/s11356-022-18990-w&rft_dat=%3Cproquest_cross%3E2625268448%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2666711061&rft_id=info:pmid/35113383&rfr_iscdi=true |