Staphylococcus aureus surface protein G (sasG) allelic variants: correlation between biofilm formation and their prevalence in methicillin-resistant S. aureus (MRSA) clones
Methicillin-resistant Staphylococcus aureus (MRSA) may persist for long periods due to biofilm formation. The objective of this study was to describe biofilm formation in association with the presence of S. aureus surface protein G (sasG) and its allelic variants in MRSA bacteraemia isolates from en...
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| Veröffentlicht in: | Research in microbiology 2022-03, Vol.173 (3), p.103921-103921, Article 103921 |
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| creator | Carrera-Salinas, Anna González-Díaz, Aida Vázquez-Sánchez, Daniel Antonio Camoez, Mariana Niubó, Jordi Càmara, Jordi Ardanuy, Carmen Martí, Sara Domínguez, M Ángeles Garcia, M. Marco, F. Chaves, F. Cercenado, E. Tapiol, J. Xercavins, M. Fontanals, D. Loza, E. Rodríguez-López, F. Olarte, I. Mirelis, B. Ruiz de Gopegui, E. Lepe, J.A. Larrosa, N. |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) may persist for long periods due to biofilm formation. The objective of this study was to describe biofilm formation in association with the presence of S. aureus surface protein G (sasG) and its allelic variants in MRSA bacteraemia isolates from endemic (CC5, CC8, CC22) and sporadic clones in Spain (2008–2015). Crystal violet staining was used to assess biofilm formation; DNA microarray, RT-qPCR, and long-read whole genome sequencing were applied to determine the presence, expression and structure of sasG, respectively. The endemic CC5 and CC8 clones produced more biofilm than the sporadic clones; these endemic clones carried sasG allelic variant 1. Otherwise, sporadic clones, with less biofilm formation, showed either an absence of sasG (65%) or the presence of allelic variant 2 (35%). Variants 1 and 2 differed in the expression of sasG (1.56 ± 1.20 and 0.37 ± 0.32, respectively). The analysis of a large cohort of closed S. aureus genomes available on the NCBI database confirmed the distribution of the two allelic variants with low amino acid identity (68.1%) among endemic and sporadic clones. SasG variant 1 present in the major CC5 and CC8 clones was correlated with increased biofilm formation and may represent an important virulence determinant. |
| doi_str_mv | 10.1016/j.resmic.2022.103921 |
| format | Article |
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The objective of this study was to describe biofilm formation in association with the presence of S. aureus surface protein G (sasG) and its allelic variants in MRSA bacteraemia isolates from endemic (CC5, CC8, CC22) and sporadic clones in Spain (2008–2015). Crystal violet staining was used to assess biofilm formation; DNA microarray, RT-qPCR, and long-read whole genome sequencing were applied to determine the presence, expression and structure of sasG, respectively. The endemic CC5 and CC8 clones produced more biofilm than the sporadic clones; these endemic clones carried sasG allelic variant 1. Otherwise, sporadic clones, with less biofilm formation, showed either an absence of sasG (65%) or the presence of allelic variant 2 (35%). Variants 1 and 2 differed in the expression of sasG (1.56 ± 1.20 and 0.37 ± 0.32, respectively). The analysis of a large cohort of closed S. aureus genomes available on the NCBI database confirmed the distribution of the two allelic variants with low amino acid identity (68.1%) among endemic and sporadic clones. SasG variant 1 present in the major CC5 and CC8 clones was correlated with increased biofilm formation and may represent an important virulence determinant.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/j.resmic.2022.103921</identifier><identifier>PMID: 35091030</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Anti-Bacterial Agents - pharmacology ; Biofilms ; Clone Cells ; Humans ; Membrane Proteins ; Methicillin Resistance ; Methicillin-Resistant Staphylococcus aureus - genetics ; Microbial Sensitivity Tests ; Prevalence ; SasG protein ; Sepsis ; Staphylococcal Infections - epidemiology ; Staphylococcus aureus</subject><ispartof>Research in microbiology, 2022-03, Vol.173 (3), p.103921-103921, Article 103921</ispartof><rights>2022 Institut Pasteur</rights><rights>Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. 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The objective of this study was to describe biofilm formation in association with the presence of S. aureus surface protein G (sasG) and its allelic variants in MRSA bacteraemia isolates from endemic (CC5, CC8, CC22) and sporadic clones in Spain (2008–2015). Crystal violet staining was used to assess biofilm formation; DNA microarray, RT-qPCR, and long-read whole genome sequencing were applied to determine the presence, expression and structure of sasG, respectively. The endemic CC5 and CC8 clones produced more biofilm than the sporadic clones; these endemic clones carried sasG allelic variant 1. Otherwise, sporadic clones, with less biofilm formation, showed either an absence of sasG (65%) or the presence of allelic variant 2 (35%). Variants 1 and 2 differed in the expression of sasG (1.56 ± 1.20 and 0.37 ± 0.32, respectively). The analysis of a large cohort of closed S. aureus genomes available on the NCBI database confirmed the distribution of the two allelic variants with low amino acid identity (68.1%) among endemic and sporadic clones. 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The objective of this study was to describe biofilm formation in association with the presence of S. aureus surface protein G (sasG) and its allelic variants in MRSA bacteraemia isolates from endemic (CC5, CC8, CC22) and sporadic clones in Spain (2008–2015). Crystal violet staining was used to assess biofilm formation; DNA microarray, RT-qPCR, and long-read whole genome sequencing were applied to determine the presence, expression and structure of sasG, respectively. The endemic CC5 and CC8 clones produced more biofilm than the sporadic clones; these endemic clones carried sasG allelic variant 1. Otherwise, sporadic clones, with less biofilm formation, showed either an absence of sasG (65%) or the presence of allelic variant 2 (35%). Variants 1 and 2 differed in the expression of sasG (1.56 ± 1.20 and 0.37 ± 0.32, respectively). The analysis of a large cohort of closed S. aureus genomes available on the NCBI database confirmed the distribution of the two allelic variants with low amino acid identity (68.1%) among endemic and sporadic clones. SasG variant 1 present in the major CC5 and CC8 clones was correlated with increased biofilm formation and may represent an important virulence determinant.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>35091030</pmid><doi>10.1016/j.resmic.2022.103921</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7517-6874</orcidid><orcidid>https://orcid.org/0000-0003-0225-607X</orcidid><orcidid>https://orcid.org/0000-0003-4205-1781</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Research in microbiology, 2022-03, Vol.173 (3), p.103921-103921, Article 103921 |
| issn | 0923-2508 1769-7123 |
| language | eng |
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| subjects | Anti-Bacterial Agents - pharmacology Biofilms Clone Cells Humans Membrane Proteins Methicillin Resistance Methicillin-Resistant Staphylococcus aureus - genetics Microbial Sensitivity Tests Prevalence SasG protein Sepsis Staphylococcal Infections - epidemiology Staphylococcus aureus |
| title | Staphylococcus aureus surface protein G (sasG) allelic variants: correlation between biofilm formation and their prevalence in methicillin-resistant S. aureus (MRSA) clones |
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