Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane
Protein-membrane interactions play important roles in signal transductions and functional regulation of membrane proteins. Here, we design a molecular dynamometer (MDM) for analyzing protein-membrane interaction on living cells. The MDM is constructed by assembling an artificial lipid bilayer and al...
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| Veröffentlicht in: | Chemical science (Cambridge) 2021-11, Vol.12 (43), p.14389-14395 |
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| creator | Liu, Huipu Chen, Yunlong Wang, Jiawei Yang, Yuanjiao Ju, Huangxian |
| description | Protein-membrane interactions play important roles in signal transductions and functional regulation of membrane proteins. Here, we design a molecular dynamometer (MDM) for analyzing protein-membrane interaction on living cells. The MDM is constructed by assembling an artificial lipid bilayer and alkylated Cy3-DNA azide (azide-Cy3-C
x
) on a silica bubble. After a functional aptamer is covalently anchored onto the corresponding target protein on a living cell through UV irradiation, azide-Cy3-C
x
is conjugated with the aptamer through a click reaction to produce a "tug-of-war" between the MDM and the cell due to the buoyancy of the silica bubble. This induces the detachment of the protein from the cell membrane or the alkane terminal from the MDM enabling sub-piconewton embedding force measurement by changing the alkane chain length and simple fluorescence analysis. The successful analysis of embedding force variation of a protein on the cell membrane upon post-translational modifications demonstrates the practicability and expansibility of this method for mechanics-related research in biological systems.
A molecular dynamometer is designed to analyze the variation of sub-piconewton interaction between a specific protein and the membrane on living cells. |
| doi_str_mv | 10.1039/d1sc03059k |
| format | Article |
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x
) on a silica bubble. After a functional aptamer is covalently anchored onto the corresponding target protein on a living cell through UV irradiation, azide-Cy3-C
x
is conjugated with the aptamer through a click reaction to produce a "tug-of-war" between the MDM and the cell due to the buoyancy of the silica bubble. This induces the detachment of the protein from the cell membrane or the alkane terminal from the MDM enabling sub-piconewton embedding force measurement by changing the alkane chain length and simple fluorescence analysis. The successful analysis of embedding force variation of a protein on the cell membrane upon post-translational modifications demonstrates the practicability and expansibility of this method for mechanics-related research in biological systems.
A molecular dynamometer is designed to analyze the variation of sub-piconewton interaction between a specific protein and the membrane on living cells.</description><identifier>ISSN: 2041-6520</identifier><identifier>EISSN: 2041-6539</identifier><identifier>DOI: 10.1039/d1sc03059k</identifier><identifier>PMID: 34880990</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Alkanes ; Alkylation ; Cell membranes ; Cells (biology) ; Chemistry ; Dynamometers ; Embedding ; Fluorescence ; Force measurement ; Lipids ; Proteins ; Silicon dioxide ; Ultraviolet radiation</subject><ispartof>Chemical science (Cambridge), 2021-11, Vol.12 (43), p.14389-14395</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><rights>This journal is © The Royal Society of Chemistry 2021 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-62446b6351fb467f700e80976162318c70a2eaf53645539326a23c5fde7726803</citedby><cites>FETCH-LOGICAL-c405t-62446b6351fb467f700e80976162318c70a2eaf53645539326a23c5fde7726803</cites><orcidid>0000-0002-6741-5302 ; 0000-0002-3775-3028</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580102/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580102/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Liu, Huipu</creatorcontrib><creatorcontrib>Chen, Yunlong</creatorcontrib><creatorcontrib>Wang, Jiawei</creatorcontrib><creatorcontrib>Yang, Yuanjiao</creatorcontrib><creatorcontrib>Ju, Huangxian</creatorcontrib><title>Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane</title><title>Chemical science (Cambridge)</title><description>Protein-membrane interactions play important roles in signal transductions and functional regulation of membrane proteins. Here, we design a molecular dynamometer (MDM) for analyzing protein-membrane interaction on living cells. The MDM is constructed by assembling an artificial lipid bilayer and alkylated Cy3-DNA azide (azide-Cy3-C
x
) on a silica bubble. After a functional aptamer is covalently anchored onto the corresponding target protein on a living cell through UV irradiation, azide-Cy3-C
x
is conjugated with the aptamer through a click reaction to produce a "tug-of-war" between the MDM and the cell due to the buoyancy of the silica bubble. This induces the detachment of the protein from the cell membrane or the alkane terminal from the MDM enabling sub-piconewton embedding force measurement by changing the alkane chain length and simple fluorescence analysis. The successful analysis of embedding force variation of a protein on the cell membrane upon post-translational modifications demonstrates the practicability and expansibility of this method for mechanics-related research in biological systems.
A molecular dynamometer is designed to analyze the variation of sub-piconewton interaction between a specific protein and the membrane on living cells.</description><subject>Alkanes</subject><subject>Alkylation</subject><subject>Cell membranes</subject><subject>Cells (biology)</subject><subject>Chemistry</subject><subject>Dynamometers</subject><subject>Embedding</subject><subject>Fluorescence</subject><subject>Force measurement</subject><subject>Lipids</subject><subject>Proteins</subject><subject>Silicon dioxide</subject><subject>Ultraviolet radiation</subject><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdklFvFCEQx4mxsU3ti-8mJL4Yk9UBFnbXB5PmtNW0iQ_WZ8JycEfdhRXYXs7v4Hcu12vOWF5mAj_-mf_MIPSKwHsCrPuwJEkDA979eoZOKNSkEpx1zw85hWN0ltItlMMY4bR5gY5Z3bbQdXCC_t7MqyrYaqPiRzyGweh5UBEvt16NYTTZxITVSjmfMh7cnfMrrM0wJGxDxMqrYftnd5fmvpqcDt5scvDY-fJR6exKHixWOE1GO-s0nmLIxnm8cXmN89o8qOHRjH1U3rxER1YNyZw9xlP08-LLzeJrdf398tvi_LrSNfBcCVrXoheME9vXorENgCl-GkEEZaTVDShqlOVM1Lw0g1GhKNPcLk3TUNECO0Wf9rrT3I9mqY3PUQ1yim5UcSuDcvL_F-_WchXuZMtbIECLwNtHgRh-zyZlObq0s1JMhDlJWgqBglJW0DdP0Nswx9K5QvGO80YAIYV6t6d0DClFYw_FEJC7QcvP5MfiYdBXBX69h2PSB-7fIrB7j16k3Q</recordid><startdate>20211110</startdate><enddate>20211110</enddate><creator>Liu, Huipu</creator><creator>Chen, Yunlong</creator><creator>Wang, Jiawei</creator><creator>Yang, Yuanjiao</creator><creator>Ju, Huangxian</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6741-5302</orcidid><orcidid>https://orcid.org/0000-0002-3775-3028</orcidid></search><sort><creationdate>20211110</creationdate><title>Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane</title><author>Liu, Huipu ; Chen, Yunlong ; Wang, Jiawei ; Yang, Yuanjiao ; Ju, Huangxian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-62446b6351fb467f700e80976162318c70a2eaf53645539326a23c5fde7726803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alkanes</topic><topic>Alkylation</topic><topic>Cell membranes</topic><topic>Cells (biology)</topic><topic>Chemistry</topic><topic>Dynamometers</topic><topic>Embedding</topic><topic>Fluorescence</topic><topic>Force measurement</topic><topic>Lipids</topic><topic>Proteins</topic><topic>Silicon dioxide</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Huipu</creatorcontrib><creatorcontrib>Chen, Yunlong</creatorcontrib><creatorcontrib>Wang, Jiawei</creatorcontrib><creatorcontrib>Yang, Yuanjiao</creatorcontrib><creatorcontrib>Ju, Huangxian</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Huipu</au><au>Chen, Yunlong</au><au>Wang, Jiawei</au><au>Yang, Yuanjiao</au><au>Ju, Huangxian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane</atitle><jtitle>Chemical science (Cambridge)</jtitle><date>2021-11-10</date><risdate>2021</risdate><volume>12</volume><issue>43</issue><spage>14389</spage><epage>14395</epage><pages>14389-14395</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>Protein-membrane interactions play important roles in signal transductions and functional regulation of membrane proteins. Here, we design a molecular dynamometer (MDM) for analyzing protein-membrane interaction on living cells. The MDM is constructed by assembling an artificial lipid bilayer and alkylated Cy3-DNA azide (azide-Cy3-C
x
) on a silica bubble. After a functional aptamer is covalently anchored onto the corresponding target protein on a living cell through UV irradiation, azide-Cy3-C
x
is conjugated with the aptamer through a click reaction to produce a "tug-of-war" between the MDM and the cell due to the buoyancy of the silica bubble. This induces the detachment of the protein from the cell membrane or the alkane terminal from the MDM enabling sub-piconewton embedding force measurement by changing the alkane chain length and simple fluorescence analysis. The successful analysis of embedding force variation of a protein on the cell membrane upon post-translational modifications demonstrates the practicability and expansibility of this method for mechanics-related research in biological systems.
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| ispartof | Chemical science (Cambridge), 2021-11, Vol.12 (43), p.14389-14395 |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Alkanes Alkylation Cell membranes Cells (biology) Chemistry Dynamometers Embedding Fluorescence Force measurement Lipids Proteins Silicon dioxide Ultraviolet radiation |
| title | Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane |
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