Association of insulin-like growth factor binding protein-7 promoter methylation with esophageal cancer in peripheral blood
Background The insulin-like growth factor (IGF) signaling pathway has an important role in many cancers, including esophageal cancer (EC). IGF-binding protein 7 (IGFBP7) is one of the proteins in this signaling pathway, and its role in cancer has not yet been fully clarified. In the present study, w...
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| Veröffentlicht in: | Molecular biology reports 2022-05, Vol.49 (5), p.3423-3431 |
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| creator | Kaya, Zehra Almalı, Necat Sahin, Elif Sena Duran, Seren Görgisen, Gökhan Ates, Can |
| description | Background
The insulin-like growth factor (IGF) signaling pathway has an important role in many cancers, including esophageal cancer (EC). IGF-binding protein 7 (IGFBP7) is one of the proteins in this signaling pathway, and its role in cancer has not yet been fully clarified. In the present study, we evaluated the clinical relevance of IGFBP7 methylation status and mRNA expression in EC patients compared to healthy controls. We also investigated whether IGFBP7 methylation status affects mRNA expression.
Methods
The study comprised 100 EC patients and 105 healthy controls. Methylation specific PCR (MSP) was used to examine IGFBP7's promoter methylation and real-time quantitative reverse transcription PCR (qRT-PCR) was used to assess IGFBP7 mRNA expression.
Results
The IGFBP7 promoter methylation was significantly higher in controls than in EC patients (p |
| doi_str_mv | 10.1007/s11033-022-07173-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2622659864</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2622659864</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-f9d3bf8a3f944a53a3feb7d3861acf8aba07c00fa959fbe7fa804cd157ea68c3</originalsourceid><addsrcrecordid>eNp9kUFv1DAQhS1ERZeWP8ABWeLCJdSO49g5VlWhSJW49G45znjXJbGDnaha9c8zbQpIHDj5afy955EfIe85-8wZUxeFcyZExeq6YoorUR1fkR2XKJpO6ddkxwTjVaMlPyVvS7lnjDVcyTfkVEimWi3rHXm8LCW5YJeQIk2ehljWMcRqDD-A7nN6WA7UW7ekTPsQhxD3dM5pAUTUk5pQZzrBcjiOW8hDQAuUNB_sHuxInY0OkRDpDDnMB8g47MeUhnNy4u1Y4N3LeUbuvlzfXd1Ut9-_fru6vK2cUHKpfDeI3msrfNc0VgoU0KtB6JZbh_PeMuUY87aTne9BeatZ4wb8CLCtduKMfNpicd2fK5TFTKE4GEcbIa3F1G1dt7LTbYPox3_Q-7TmiMshpYTiXHGNVL1RLqdSMngz5zDZfDScmadmzNaMwWbMczPmiKYPL9FrP8Hwx_K7CgTEBhS8invIf9_-T-wv5Dec9Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2673711718</pqid></control><display><type>article</type><title>Association of insulin-like growth factor binding protein-7 promoter methylation with esophageal cancer in peripheral blood</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Kaya, Zehra ; Almalı, Necat ; Sahin, Elif Sena ; Duran, Seren ; Görgisen, Gökhan ; Ates, Can</creator><creatorcontrib>Kaya, Zehra ; Almalı, Necat ; Sahin, Elif Sena ; Duran, Seren ; Görgisen, Gökhan ; Ates, Can</creatorcontrib><description>Background
The insulin-like growth factor (IGF) signaling pathway has an important role in many cancers, including esophageal cancer (EC). IGF-binding protein 7 (IGFBP7) is one of the proteins in this signaling pathway, and its role in cancer has not yet been fully clarified. In the present study, we evaluated the clinical relevance of IGFBP7 methylation status and mRNA expression in EC patients compared to healthy controls. We also investigated whether IGFBP7 methylation status affects mRNA expression.
Methods
The study comprised 100 EC patients and 105 healthy controls. Methylation specific PCR (MSP) was used to examine IGFBP7's promoter methylation and real-time quantitative reverse transcription PCR (qRT-PCR) was used to assess IGFBP7 mRNA expression.
Results
The IGFBP7 promoter methylation was significantly higher in controls than in EC patients (p < 0.05). IGFBP7 mRNA expression was significantly lower in EC patients compared to controls, especially in those over 55 years old (p < 0.0001). The globulin level and reflux were significantly higher in IGFBP7-unmethylated patients compared to IGFBP7 methylated patients (p = 0.01). In EC patients, however, there was no significant relationship between IGFBP7 mRNA expression and methylation in the peripheral blood (p = 0.33). In addition, neither IGFBP7 mRNA expression nor methylation were shown to be linked with survival (p > 0.05).
Conclusion
Our study indicated that promoter unmethylation and mRNA expression of the IGFBP7 promoter in peripheral blood could be different biomarkers for EC. Furthermore, unmethylation of the IGFBP7 promoter in EC patients was associated with reflux and elevated globulin levels. More studies with a larger number of cases is needed to confirm this association.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07173-y</identifier><identifier>PMID: 35076852</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Cancer ; DNA methylation ; DNA Methylation - genetics ; Esophageal cancer ; Esophageal Neoplasms - genetics ; Gene expression ; Globulins ; Globulins - genetics ; Histology ; Humans ; Insulin ; Insulin-Like Growth Factor Binding Proteins - genetics ; Insulin-Like Growth Factor Binding Proteins - metabolism ; Insulin-like growth factors ; Life Sciences ; Middle Aged ; Morphology ; Original Article ; Patients ; Peripheral blood ; Reverse transcription ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal transduction</subject><ispartof>Molecular biology reports, 2022-05, Vol.49 (5), p.3423-3431</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f9d3bf8a3f944a53a3feb7d3861acf8aba07c00fa959fbe7fa804cd157ea68c3</citedby><cites>FETCH-LOGICAL-c375t-f9d3bf8a3f944a53a3feb7d3861acf8aba07c00fa959fbe7fa804cd157ea68c3</cites><orcidid>0000-0001-6222-7882</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-022-07173-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-022-07173-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35076852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaya, Zehra</creatorcontrib><creatorcontrib>Almalı, Necat</creatorcontrib><creatorcontrib>Sahin, Elif Sena</creatorcontrib><creatorcontrib>Duran, Seren</creatorcontrib><creatorcontrib>Görgisen, Gökhan</creatorcontrib><creatorcontrib>Ates, Can</creatorcontrib><title>Association of insulin-like growth factor binding protein-7 promoter methylation with esophageal cancer in peripheral blood</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
The insulin-like growth factor (IGF) signaling pathway has an important role in many cancers, including esophageal cancer (EC). IGF-binding protein 7 (IGFBP7) is one of the proteins in this signaling pathway, and its role in cancer has not yet been fully clarified. In the present study, we evaluated the clinical relevance of IGFBP7 methylation status and mRNA expression in EC patients compared to healthy controls. We also investigated whether IGFBP7 methylation status affects mRNA expression.
Methods
The study comprised 100 EC patients and 105 healthy controls. Methylation specific PCR (MSP) was used to examine IGFBP7's promoter methylation and real-time quantitative reverse transcription PCR (qRT-PCR) was used to assess IGFBP7 mRNA expression.
Results
The IGFBP7 promoter methylation was significantly higher in controls than in EC patients (p < 0.05). IGFBP7 mRNA expression was significantly lower in EC patients compared to controls, especially in those over 55 years old (p < 0.0001). The globulin level and reflux were significantly higher in IGFBP7-unmethylated patients compared to IGFBP7 methylated patients (p = 0.01). In EC patients, however, there was no significant relationship between IGFBP7 mRNA expression and methylation in the peripheral blood (p = 0.33). In addition, neither IGFBP7 mRNA expression nor methylation were shown to be linked with survival (p > 0.05).
Conclusion
Our study indicated that promoter unmethylation and mRNA expression of the IGFBP7 promoter in peripheral blood could be different biomarkers for EC. Furthermore, unmethylation of the IGFBP7 promoter in EC patients was associated with reflux and elevated globulin levels. More studies with a larger number of cases is needed to confirm this association.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer</subject><subject>DNA methylation</subject><subject>DNA Methylation - genetics</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Gene expression</subject><subject>Globulins</subject><subject>Globulins - genetics</subject><subject>Histology</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin-Like Growth Factor Binding Proteins - genetics</subject><subject>Insulin-Like Growth Factor Binding Proteins - metabolism</subject><subject>Insulin-like growth factors</subject><subject>Life Sciences</subject><subject>Middle Aged</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Patients</subject><subject>Peripheral blood</subject><subject>Reverse transcription</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal transduction</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUFv1DAQhS1ERZeWP8ABWeLCJdSO49g5VlWhSJW49G45znjXJbGDnaha9c8zbQpIHDj5afy955EfIe85-8wZUxeFcyZExeq6YoorUR1fkR2XKJpO6ddkxwTjVaMlPyVvS7lnjDVcyTfkVEimWi3rHXm8LCW5YJeQIk2ehljWMcRqDD-A7nN6WA7UW7ekTPsQhxD3dM5pAUTUk5pQZzrBcjiOW8hDQAuUNB_sHuxInY0OkRDpDDnMB8g47MeUhnNy4u1Y4N3LeUbuvlzfXd1Ut9-_fru6vK2cUHKpfDeI3msrfNc0VgoU0KtB6JZbh_PeMuUY87aTne9BeatZ4wb8CLCtduKMfNpicd2fK5TFTKE4GEcbIa3F1G1dt7LTbYPox3_Q-7TmiMshpYTiXHGNVL1RLqdSMngz5zDZfDScmadmzNaMwWbMczPmiKYPL9FrP8Hwx_K7CgTEBhS8invIf9_-T-wv5Dec9Q</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Kaya, Zehra</creator><creator>Almalı, Necat</creator><creator>Sahin, Elif Sena</creator><creator>Duran, Seren</creator><creator>Görgisen, Gökhan</creator><creator>Ates, Can</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6222-7882</orcidid></search><sort><creationdate>20220501</creationdate><title>Association of insulin-like growth factor binding protein-7 promoter methylation with esophageal cancer in peripheral blood</title><author>Kaya, Zehra ; Almalı, Necat ; Sahin, Elif Sena ; Duran, Seren ; Görgisen, Gökhan ; Ates, Can</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f9d3bf8a3f944a53a3feb7d3861acf8aba07c00fa959fbe7fa804cd157ea68c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cancer</topic><topic>DNA methylation</topic><topic>DNA Methylation - genetics</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Gene expression</topic><topic>Globulins</topic><topic>Globulins - genetics</topic><topic>Histology</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin-Like Growth Factor Binding Proteins - genetics</topic><topic>Insulin-Like Growth Factor Binding Proteins - metabolism</topic><topic>Insulin-like growth factors</topic><topic>Life Sciences</topic><topic>Middle Aged</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Patients</topic><topic>Peripheral blood</topic><topic>Reverse transcription</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaya, Zehra</creatorcontrib><creatorcontrib>Almalı, Necat</creatorcontrib><creatorcontrib>Sahin, Elif Sena</creatorcontrib><creatorcontrib>Duran, Seren</creatorcontrib><creatorcontrib>Görgisen, Gökhan</creatorcontrib><creatorcontrib>Ates, Can</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaya, Zehra</au><au>Almalı, Necat</au><au>Sahin, Elif Sena</au><au>Duran, Seren</au><au>Görgisen, Gökhan</au><au>Ates, Can</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of insulin-like growth factor binding protein-7 promoter methylation with esophageal cancer in peripheral blood</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>49</volume><issue>5</issue><spage>3423</spage><epage>3431</epage><pages>3423-3431</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
The insulin-like growth factor (IGF) signaling pathway has an important role in many cancers, including esophageal cancer (EC). IGF-binding protein 7 (IGFBP7) is one of the proteins in this signaling pathway, and its role in cancer has not yet been fully clarified. In the present study, we evaluated the clinical relevance of IGFBP7 methylation status and mRNA expression in EC patients compared to healthy controls. We also investigated whether IGFBP7 methylation status affects mRNA expression.
Methods
The study comprised 100 EC patients and 105 healthy controls. Methylation specific PCR (MSP) was used to examine IGFBP7's promoter methylation and real-time quantitative reverse transcription PCR (qRT-PCR) was used to assess IGFBP7 mRNA expression.
Results
The IGFBP7 promoter methylation was significantly higher in controls than in EC patients (p < 0.05). IGFBP7 mRNA expression was significantly lower in EC patients compared to controls, especially in those over 55 years old (p < 0.0001). The globulin level and reflux were significantly higher in IGFBP7-unmethylated patients compared to IGFBP7 methylated patients (p = 0.01). In EC patients, however, there was no significant relationship between IGFBP7 mRNA expression and methylation in the peripheral blood (p = 0.33). In addition, neither IGFBP7 mRNA expression nor methylation were shown to be linked with survival (p > 0.05).
Conclusion
Our study indicated that promoter unmethylation and mRNA expression of the IGFBP7 promoter in peripheral blood could be different biomarkers for EC. Furthermore, unmethylation of the IGFBP7 promoter in EC patients was associated with reflux and elevated globulin levels. More studies with a larger number of cases is needed to confirm this association.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>35076852</pmid><doi>10.1007/s11033-022-07173-y</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6222-7882</orcidid></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Cancer DNA methylation DNA Methylation - genetics Esophageal cancer Esophageal Neoplasms - genetics Gene expression Globulins Globulins - genetics Histology Humans Insulin Insulin-Like Growth Factor Binding Proteins - genetics Insulin-Like Growth Factor Binding Proteins - metabolism Insulin-like growth factors Life Sciences Middle Aged Morphology Original Article Patients Peripheral blood Reverse transcription RNA, Messenger - genetics RNA, Messenger - metabolism Signal transduction |
| title | Association of insulin-like growth factor binding protein-7 promoter methylation with esophageal cancer in peripheral blood |
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