Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility
VITEK®2, MALDI-TOF MS and 16S rRNA sequencing were evaluated for the identification of aerobic endospore-forming bacteria (AEB) from a pharmaceutical facility. MALDI-TOF MS demonstrated higher accuracy compared to VITEK®2, although both databases were insufficient to identify AEB species. Sequencing...
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| Veröffentlicht in: | Journal of microbiological methods 2022-03, Vol.194, p.106419-106419, Article 106419 |
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| container_title | Journal of microbiological methods |
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| creator | Costa, Luciana Veloso da Miranda, Rebeca Vitoria da Silva Lage de Fonseca, Erica Louro da Gonçalves, Natalia Pedra Reis, Cristhiane Moura Falavina dos Frazão, Adriana Marques Cruz, Fernanda Ventura Brandão, Marcelo Luiz Lima Ramos, Juliana Nunes Vieira, Verônica Viana |
| description | VITEK®2, MALDI-TOF MS and 16S rRNA sequencing were evaluated for the identification of aerobic endospore-forming bacteria (AEB) from a pharmaceutical facility. MALDI-TOF MS demonstrated higher accuracy compared to VITEK®2, although both databases were insufficient to identify AEB species. Sequencing was the best methodology, but unable to identify closely related species.
[Display omitted]
•VITEK® 2 was not effective for AEB identification.•VITEK® 2 and MALDI-TOF MS databases are not updated with most AEB species.1•16S rRNA sequencing was the best method for AEB identification.•Bacillus and Paenibacillus were the main isolated genera.•Five possible new species were isolated. |
| doi_str_mv | 10.1016/j.mimet.2022.106419 |
| format | Article |
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[Display omitted]
•VITEK® 2 was not effective for AEB identification.•VITEK® 2 and MALDI-TOF MS databases are not updated with most AEB species.1•16S rRNA sequencing was the best method for AEB identification.•Bacillus and Paenibacillus were the main isolated genera.•Five possible new species were isolated.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2022.106419</identifier><identifier>PMID: 35074480</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>16S rRNA ; Bacterial identification ; MALDI-TOF MS ; Pharmaceutical industry ; Phenotypic characterization</subject><ispartof>Journal of microbiological methods, 2022-03, Vol.194, p.106419-106419, Article 106419</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-47b0bd4478f826abb7ca8218fd5b4b7dda3ea92125a25654e98f7a4e1c05f1a03</citedby><cites>FETCH-LOGICAL-c359t-47b0bd4478f826abb7ca8218fd5b4b7dda3ea92125a25654e98f7a4e1c05f1a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2022.106419$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35074480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Costa, Luciana Veloso da</creatorcontrib><creatorcontrib>Miranda, Rebeca Vitoria da Silva Lage de</creatorcontrib><creatorcontrib>Fonseca, Erica Louro da</creatorcontrib><creatorcontrib>Gonçalves, Natalia Pedra</creatorcontrib><creatorcontrib>Reis, Cristhiane Moura Falavina dos</creatorcontrib><creatorcontrib>Frazão, Adriana Marques</creatorcontrib><creatorcontrib>Cruz, Fernanda Ventura</creatorcontrib><creatorcontrib>Brandão, Marcelo Luiz Lima</creatorcontrib><creatorcontrib>Ramos, Juliana Nunes</creatorcontrib><creatorcontrib>Vieira, Verônica Viana</creatorcontrib><title>Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>VITEK®2, MALDI-TOF MS and 16S rRNA sequencing were evaluated for the identification of aerobic endospore-forming bacteria (AEB) from a pharmaceutical facility. MALDI-TOF MS demonstrated higher accuracy compared to VITEK®2, although both databases were insufficient to identify AEB species. Sequencing was the best methodology, but unable to identify closely related species.
[Display omitted]
•VITEK® 2 was not effective for AEB identification.•VITEK® 2 and MALDI-TOF MS databases are not updated with most AEB species.1•16S rRNA sequencing was the best method for AEB identification.•Bacillus and Paenibacillus were the main isolated genera.•Five possible new species were isolated.</description><subject>16S rRNA</subject><subject>Bacterial identification</subject><subject>MALDI-TOF MS</subject><subject>Pharmaceutical industry</subject><subject>Phenotypic characterization</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhi0EotvCEyAhHzmQre04sffAYVVaWHVLJbpwtSbOuHiVxIudIPVF-hg8BE-Gly099mRp_M2M_vkIecPZnDNen27nve9xnAsmRK7Uki-ekRnXShS6rBbPySxTqlCMiyNynNKWMV6VUr8kR2XFlJSazcj9MiVMqcdhpMHR76vN-eWf31S8p1fL9cdVsbm-oFc3FIaWuqnr6C0OSHl9Q-PXL0ua8OeEg_XDLXUhUsAYGm8pDm1IuxCxyNV-_9uAHTF6oD6FDkbM02LoKdDdD4g9WJxGb6GjDqzv_Hj3irxw0CV8_fCekG8X55uzz8X6-tPqbLkubE44FlI1rGmlVNppUUPTKAtacO3aqpGNalsoERaCiwpEVVcSF9opkMgtqxwHVp6Qd4e5uxhylDSa3ieLXQcDhikZUQtRV0pIntHygNoYUorozC76HuKd4czshZit-SfE7IWYg5Dc9fZhwdT02D72_DeQgQ8HAHPMXx6jSdbnm2LrI9rRtME_ueAvNnKd1w</recordid><startdate>202203</startdate><enddate>202203</enddate><creator>Costa, Luciana Veloso da</creator><creator>Miranda, Rebeca Vitoria da Silva Lage de</creator><creator>Fonseca, Erica Louro da</creator><creator>Gonçalves, Natalia Pedra</creator><creator>Reis, Cristhiane Moura Falavina dos</creator><creator>Frazão, Adriana Marques</creator><creator>Cruz, Fernanda Ventura</creator><creator>Brandão, Marcelo Luiz Lima</creator><creator>Ramos, Juliana Nunes</creator><creator>Vieira, Verônica Viana</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202203</creationdate><title>Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility</title><author>Costa, Luciana Veloso da ; Miranda, Rebeca Vitoria da Silva Lage de ; Fonseca, Erica Louro da ; Gonçalves, Natalia Pedra ; Reis, Cristhiane Moura Falavina dos ; Frazão, Adriana Marques ; Cruz, Fernanda Ventura ; Brandão, Marcelo Luiz Lima ; Ramos, Juliana Nunes ; Vieira, Verônica Viana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-47b0bd4478f826abb7ca8218fd5b4b7dda3ea92125a25654e98f7a4e1c05f1a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>16S rRNA</topic><topic>Bacterial identification</topic><topic>MALDI-TOF MS</topic><topic>Pharmaceutical industry</topic><topic>Phenotypic characterization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Costa, Luciana Veloso da</creatorcontrib><creatorcontrib>Miranda, Rebeca Vitoria da Silva Lage de</creatorcontrib><creatorcontrib>Fonseca, Erica Louro da</creatorcontrib><creatorcontrib>Gonçalves, Natalia Pedra</creatorcontrib><creatorcontrib>Reis, Cristhiane Moura Falavina dos</creatorcontrib><creatorcontrib>Frazão, Adriana Marques</creatorcontrib><creatorcontrib>Cruz, Fernanda Ventura</creatorcontrib><creatorcontrib>Brandão, Marcelo Luiz Lima</creatorcontrib><creatorcontrib>Ramos, Juliana Nunes</creatorcontrib><creatorcontrib>Vieira, Verônica Viana</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Costa, Luciana Veloso da</au><au>Miranda, Rebeca Vitoria da Silva Lage de</au><au>Fonseca, Erica Louro da</au><au>Gonçalves, Natalia Pedra</au><au>Reis, Cristhiane Moura Falavina dos</au><au>Frazão, Adriana Marques</au><au>Cruz, Fernanda Ventura</au><au>Brandão, Marcelo Luiz Lima</au><au>Ramos, Juliana Nunes</au><au>Vieira, Verônica Viana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2022-03</date><risdate>2022</risdate><volume>194</volume><spage>106419</spage><epage>106419</epage><pages>106419-106419</pages><artnum>106419</artnum><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>VITEK®2, MALDI-TOF MS and 16S rRNA sequencing were evaluated for the identification of aerobic endospore-forming bacteria (AEB) from a pharmaceutical facility. MALDI-TOF MS demonstrated higher accuracy compared to VITEK®2, although both databases were insufficient to identify AEB species. Sequencing was the best methodology, but unable to identify closely related species.
[Display omitted]
•VITEK® 2 was not effective for AEB identification.•VITEK® 2 and MALDI-TOF MS databases are not updated with most AEB species.1•16S rRNA sequencing was the best method for AEB identification.•Bacillus and Paenibacillus were the main isolated genera.•Five possible new species were isolated.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>35074480</pmid><doi>10.1016/j.mimet.2022.106419</doi><tpages>1</tpages></addata></record> |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | 16S rRNA Bacterial identification MALDI-TOF MS Pharmaceutical industry Phenotypic characterization |
| title | Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility |
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