A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively
Ca 2 + plays an important role as an intracellular second messenger in the growth and development of cardiomyocytes (CMs), which can be visualized by calcium imaging and be quantified as calcium transient. Based on calcium imaging, the widely applied measurement method for cellular calcium transient...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2022-05, Vol.194 (5), p.2236-2250 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Gao, Lu Ye, Jing Xiao, Zhenghua He, Ling Zhang, Jing Zhang, Han Yang, Gang |
| description | Ca
2
+
plays an important role as an intracellular second messenger in the growth and development of cardiomyocytes (CMs), which can be visualized by calcium imaging and be quantified as calcium transient. Based on calcium imaging, the widely applied measurement method for cellular calcium transient requires laborious and inefficient calibration experiments, as well as affected by photobleaching. In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% of the image area. This result indicated that the new method provided a new concept of cellular
Ca
2
+
concentration quantification as well as a rapid and adaptive method for monitoring cellular calcium transient. |
| doi_str_mv | 10.1007/s12010-021-03771-x |
| format | Article |
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2
+
plays an important role as an intracellular second messenger in the growth and development of cardiomyocytes (CMs), which can be visualized by calcium imaging and be quantified as calcium transient. Based on calcium imaging, the widely applied measurement method for cellular calcium transient requires laborious and inefficient calibration experiments, as well as affected by photobleaching. In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% of the image area. This result indicated that the new method provided a new concept of cellular
Ca
2
+
concentration quantification as well as a rapid and adaptive method for monitoring cellular calcium transient.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-021-03771-x</identifier><identifier>PMID: 35066748</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biotechnology ; Calcium ; Calcium imaging ; Calcium ions ; Calibration ; Cardiomyocytes ; Chemistry ; Chemistry and Materials Science ; Correlation coefficient ; Correlation coefficients ; Image acquisition ; Image processing ; Mathematical analysis ; Measurement methods ; Monitoring ; Monitoring methods ; Neonates ; Original Article ; Photobleaching ; Photochemical reactions</subject><ispartof>Applied biochemistry and biotechnology, 2022-05, Vol.194 (5), p.2236-2250</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-c9ccd31566fb6adc3836f682fbdc5b25d69b3d5dc7cdf06f394a75d9108481013</cites><orcidid>0000-0002-2353-895X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-021-03771-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-021-03771-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35066748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Lu</creatorcontrib><creatorcontrib>Ye, Jing</creatorcontrib><creatorcontrib>Xiao, Zhenghua</creatorcontrib><creatorcontrib>He, Ling</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Zhang, Han</creatorcontrib><creatorcontrib>Yang, Gang</creatorcontrib><title>A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Ca
2
+
plays an important role as an intracellular second messenger in the growth and development of cardiomyocytes (CMs), which can be visualized by calcium imaging and be quantified as calcium transient. Based on calcium imaging, the widely applied measurement method for cellular calcium transient requires laborious and inefficient calibration experiments, as well as affected by photobleaching. In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% of the image area. This result indicated that the new method provided a new concept of cellular
Ca
2
+
concentration quantification as well as a rapid and adaptive method for monitoring cellular calcium transient.</description><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Calcium</subject><subject>Calcium imaging</subject><subject>Calcium ions</subject><subject>Calibration</subject><subject>Cardiomyocytes</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Image acquisition</subject><subject>Image processing</subject><subject>Mathematical analysis</subject><subject>Measurement methods</subject><subject>Monitoring</subject><subject>Monitoring methods</subject><subject>Neonates</subject><subject>Original Article</subject><subject>Photobleaching</subject><subject>Photochemical reactions</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LAzEURYMotlb_gAsZcONmNB-TZLIsg1WhxU1dh0ySkZT5qMmM2H9v6lQFF64ePM6973EAuETwFkHI7wLCEMEUYpRCwjlKP47AFFEq4kqgYzCFmJMU41xMwFkIGwgRzik_BRNCIWM8y6dgPU8KVbvSq951bbrw1iYrq8LgbWPbPqk6n6y61vWdd-1rUti6Hmrl9yHthiZZe9UGF8mQzI3a9u7d1rtzcFKpOtiLw5yBl8X9unhMl88PT8V8mWqCWZ9qobUhiDJWlUwZTXLCKpbjqjSalpgaJkpiqNFcmwqyiohMcWoEgnmWI4jIDNyMvVvfvQ029LJxQccXVWu7IUjMMM44YxmM6PUfdNMNvo3fRYoKIkR0Eik8Utp3IXhbya13jfI7iaDcO5ejcxmdyy_n8iOGrg7VQ9lY8xP5lhwBMgJhu5do_e_tf2o_AZBQjMs</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Gao, Lu</creator><creator>Ye, Jing</creator><creator>Xiao, Zhenghua</creator><creator>He, Ling</creator><creator>Zhang, Jing</creator><creator>Zhang, Han</creator><creator>Yang, Gang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2353-895X</orcidid></search><sort><creationdate>20220501</creationdate><title>A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively</title><author>Gao, Lu ; Ye, Jing ; Xiao, Zhenghua ; He, Ling ; Zhang, Jing ; Zhang, Han ; Yang, Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-c9ccd31566fb6adc3836f682fbdc5b25d69b3d5dc7cdf06f394a75d9108481013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Calcium</topic><topic>Calcium imaging</topic><topic>Calcium ions</topic><topic>Calibration</topic><topic>Cardiomyocytes</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Image acquisition</topic><topic>Image processing</topic><topic>Mathematical analysis</topic><topic>Measurement methods</topic><topic>Monitoring</topic><topic>Monitoring methods</topic><topic>Neonates</topic><topic>Original Article</topic><topic>Photobleaching</topic><topic>Photochemical reactions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Lu</creatorcontrib><creatorcontrib>Ye, Jing</creatorcontrib><creatorcontrib>Xiao, Zhenghua</creatorcontrib><creatorcontrib>He, Ling</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Zhang, Han</creatorcontrib><creatorcontrib>Yang, Gang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Lu</au><au>Ye, Jing</au><au>Xiao, Zhenghua</au><au>He, Ling</au><au>Zhang, Jing</au><au>Zhang, Han</au><au>Yang, Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>194</volume><issue>5</issue><spage>2236</spage><epage>2250</epage><pages>2236-2250</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Ca
2
+
plays an important role as an intracellular second messenger in the growth and development of cardiomyocytes (CMs), which can be visualized by calcium imaging and be quantified as calcium transient. Based on calcium imaging, the widely applied measurement method for cellular calcium transient requires laborious and inefficient calibration experiments, as well as affected by photobleaching. In this study, we presented a calibration-free method, based on calcium imaging, to calculate cellular calcium transient and correct photobleaching directly from the target video. We also set up image acquisition and calculation system on custom software, applied to calcium transients monitoring of neonatal rat cardiomyocytes. Results showed that the effect of the new method was similar to that of the traditional one with a Pearson correlation coefficient of 0.99 ± 0.01. Moreover, the residual sum of squares of the two methods was only 26.31 ± 26.28 when the area of the region of interest was greater than 8% of the image area. This result indicated that the new method provided a new concept of cellular
Ca
2
+
concentration quantification as well as a rapid and adaptive method for monitoring cellular calcium transient.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35066748</pmid><doi>10.1007/s12010-021-03771-x</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-2353-895X</orcidid></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2022-05, Vol.194 (5), p.2236-2250 |
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| subjects | Biochemistry Biotechnology Calcium Calcium imaging Calcium ions Calibration Cardiomyocytes Chemistry Chemistry and Materials Science Correlation coefficient Correlation coefficients Image acquisition Image processing Mathematical analysis Measurement methods Monitoring Monitoring methods Neonates Original Article Photobleaching Photochemical reactions |
| title | A Calibration-Free Measurement for Monitoring Cellular Calcium Transients Adaptively |
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