Repurposing the Antibacterial Activity of EtoposideA Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite
Drug repurposing has been gaining increasing interest recently due to the reduction in development cost and reduced development timelines. Here, we report the antibacterial activity of the anticancer drug etoposide investigated in combination with the eggshell-derived hydroxyapatite (EHA). Hydroxyap...
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| Veröffentlicht in: | ACS biomaterials science & engineering 2022-02, Vol.8 (2), p.682-693 |
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| creator | Ganesan, Vidhya Meiyazhagan, Gowri Devaraj, Muthu Kandasamy, Sangeetha Manogaran, Prasath Suresh Kumar, Govindan Raji, Govindan Kattimani, Vivekanand S Easwaradas Kreedapathy, Girija |
| description | Drug repurposing has been gaining increasing interest recently due to the reduction in development cost and reduced development timelines. Here, we report the antibacterial activity of the anticancer drug etoposide investigated in combination with the eggshell-derived hydroxyapatite (EHA). Hydroxyapatite (HA) is a well-known bioactive material with enhanced osteoconductivity and possesses superior drug delivery properties. In the present work, we have synthesized etoposide-loaded EHA by the wet precipitation method. The physicochemical characterization of the samples confirmed the composition and amount of drug encapsulation. Screening for antibacterial activity confirmed the antibacterial effect of etoposide against Staphylococcus aureus. Biofilm formation test on pristine and etoposide-loaded samples showed the inhibition of biofilm formation on etoposide loading, which was further studied by confocal laser scanning microscopy (CLSM) and colony forming units (CFUs). It has been found that etoposide-loaded HA exhibited a sustained release of the drug upto 168 h. Analysis of the inhibition mechanism of etoposide against S. aureus revealed damage to the cell membrane and has been quantified using flow cytometry by the uptake of propidium iodide. Etoposide-loaded eggshell-derived HA (EHA-ET) exhibited excellent bioactivity and cytocompatibility against mouse fibroblast cells (L929) and supressed the growth of osteosarcoma cells (MG-63). Our studies reveal that the EHA-ET has a great potential for treating osteosarcoma and osteomyelitis. |
| doi_str_mv | 10.1021/acsbiomaterials.1c01481 |
| format | Article |
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Here, we report the antibacterial activity of the anticancer drug etoposide investigated in combination with the eggshell-derived hydroxyapatite (EHA). Hydroxyapatite (HA) is a well-known bioactive material with enhanced osteoconductivity and possesses superior drug delivery properties. In the present work, we have synthesized etoposide-loaded EHA by the wet precipitation method. The physicochemical characterization of the samples confirmed the composition and amount of drug encapsulation. Screening for antibacterial activity confirmed the antibacterial effect of etoposide against Staphylococcus aureus. Biofilm formation test on pristine and etoposide-loaded samples showed the inhibition of biofilm formation on etoposide loading, which was further studied by confocal laser scanning microscopy (CLSM) and colony forming units (CFUs). It has been found that etoposide-loaded HA exhibited a sustained release of the drug upto 168 h. Analysis of the inhibition mechanism of etoposide against S. aureus revealed damage to the cell membrane and has been quantified using flow cytometry by the uptake of propidium iodide. Etoposide-loaded eggshell-derived HA (EHA-ET) exhibited excellent bioactivity and cytocompatibility against mouse fibroblast cells (L929) and supressed the growth of osteosarcoma cells (MG-63). Our studies reveal that the EHA-ET has a great potential for treating osteosarcoma and osteomyelitis.</description><identifier>ISSN: 2373-9878</identifier><identifier>EISSN: 2373-9878</identifier><identifier>DOI: 10.1021/acsbiomaterials.1c01481</identifier><identifier>PMID: 35050575</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Bio-interactions and Biocompatibility ; Drug Repositioning ; Durapatite - chemistry ; Durapatite - pharmacology ; Egg Shell ; Mice ; Staphylococcus aureus</subject><ispartof>ACS biomaterials science & engineering, 2022-02, Vol.8 (2), p.682-693</ispartof><rights>2022 American Chemical Society</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a357t-584e381d6301452298ed8a23e3b4187a0d3b7cae6762d2b36ffa2740dbf002de3</citedby><cites>FETCH-LOGICAL-a357t-584e381d6301452298ed8a23e3b4187a0d3b7cae6762d2b36ffa2740dbf002de3</cites><orcidid>0000-0002-3528-2082 ; 0000-0003-4441-6107</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsbiomaterials.1c01481$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsbiomaterials.1c01481$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35050575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ganesan, Vidhya</creatorcontrib><creatorcontrib>Meiyazhagan, Gowri</creatorcontrib><creatorcontrib>Devaraj, Muthu</creatorcontrib><creatorcontrib>Kandasamy, Sangeetha</creatorcontrib><creatorcontrib>Manogaran, Prasath</creatorcontrib><creatorcontrib>Suresh Kumar, Govindan</creatorcontrib><creatorcontrib>Raji, Govindan</creatorcontrib><creatorcontrib>Kattimani, Vivekanand S</creatorcontrib><creatorcontrib>Easwaradas Kreedapathy, Girija</creatorcontrib><title>Repurposing the Antibacterial Activity of EtoposideA Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite</title><title>ACS biomaterials science & engineering</title><addtitle>ACS Biomater. Sci. Eng</addtitle><description>Drug repurposing has been gaining increasing interest recently due to the reduction in development cost and reduced development timelines. Here, we report the antibacterial activity of the anticancer drug etoposide investigated in combination with the eggshell-derived hydroxyapatite (EHA). Hydroxyapatite (HA) is a well-known bioactive material with enhanced osteoconductivity and possesses superior drug delivery properties. In the present work, we have synthesized etoposide-loaded EHA by the wet precipitation method. The physicochemical characterization of the samples confirmed the composition and amount of drug encapsulation. Screening for antibacterial activity confirmed the antibacterial effect of etoposide against Staphylococcus aureus. Biofilm formation test on pristine and etoposide-loaded samples showed the inhibition of biofilm formation on etoposide loading, which was further studied by confocal laser scanning microscopy (CLSM) and colony forming units (CFUs). It has been found that etoposide-loaded HA exhibited a sustained release of the drug upto 168 h. Analysis of the inhibition mechanism of etoposide against S. aureus revealed damage to the cell membrane and has been quantified using flow cytometry by the uptake of propidium iodide. Etoposide-loaded eggshell-derived HA (EHA-ET) exhibited excellent bioactivity and cytocompatibility against mouse fibroblast cells (L929) and supressed the growth of osteosarcoma cells (MG-63). Our studies reveal that the EHA-ET has a great potential for treating osteosarcoma and osteomyelitis.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bio-interactions and Biocompatibility</subject><subject>Drug Repositioning</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Egg Shell</subject><subject>Mice</subject><subject>Staphylococcus aureus</subject><issn>2373-9878</issn><issn>2373-9878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtOwzAURS0EAgRsATxkkuJPErvDqpSPVAkJwThy4pfWqImD7QAdsgq2wqpYAy4tCDFBHtiDc4_9fBE6oWRACaNnqvKlsY0K4Ixa-AGtCE0l3UL7jAueDKWQ27_Oe-jI-wdCCOUyS9N0F-3xjMQlsn30egtd7zrrTTvDYQ541AZTqmrtxqMqmCcTltjWeBLsitPw8fY-wuM5NDYGnOqgD6bC566fYdPisW1K06pgbIufTZjjyWzm57BYJOfR-QQaXy21sy9L1UUowCHaqeMUcLTZD9D9xeRufJVMby6vx6NpongmQpLJFLikOudx2IyxoQQtFePAy5RKoYjmpagU5CJnmpU8r2vFREp0WRPCNPADdLr2ds4-9uBD0RhfxXepFmzvC5YzxoQcZjKiYo1WznrvoC46ZxrllgUlxaqC4k8FxaaCmDzeXNKXDeif3PeHR4CvgWgoHmzv2q_4P9pPa5Ccqw</recordid><startdate>20220214</startdate><enddate>20220214</enddate><creator>Ganesan, Vidhya</creator><creator>Meiyazhagan, Gowri</creator><creator>Devaraj, Muthu</creator><creator>Kandasamy, Sangeetha</creator><creator>Manogaran, Prasath</creator><creator>Suresh Kumar, Govindan</creator><creator>Raji, Govindan</creator><creator>Kattimani, Vivekanand S</creator><creator>Easwaradas Kreedapathy, Girija</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3528-2082</orcidid><orcidid>https://orcid.org/0000-0003-4441-6107</orcidid></search><sort><creationdate>20220214</creationdate><title>Repurposing the Antibacterial Activity of EtoposideA Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite</title><author>Ganesan, Vidhya ; Meiyazhagan, Gowri ; Devaraj, Muthu ; Kandasamy, Sangeetha ; Manogaran, Prasath ; Suresh Kumar, Govindan ; Raji, Govindan ; Kattimani, Vivekanand S ; Easwaradas Kreedapathy, Girija</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a357t-584e381d6301452298ed8a23e3b4187a0d3b7cae6762d2b36ffa2740dbf002de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bio-interactions and Biocompatibility</topic><topic>Drug Repositioning</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Egg Shell</topic><topic>Mice</topic><topic>Staphylococcus aureus</topic><toplevel>online_resources</toplevel><creatorcontrib>Ganesan, Vidhya</creatorcontrib><creatorcontrib>Meiyazhagan, Gowri</creatorcontrib><creatorcontrib>Devaraj, Muthu</creatorcontrib><creatorcontrib>Kandasamy, Sangeetha</creatorcontrib><creatorcontrib>Manogaran, Prasath</creatorcontrib><creatorcontrib>Suresh Kumar, Govindan</creatorcontrib><creatorcontrib>Raji, Govindan</creatorcontrib><creatorcontrib>Kattimani, Vivekanand S</creatorcontrib><creatorcontrib>Easwaradas Kreedapathy, Girija</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS biomaterials science & engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ganesan, Vidhya</au><au>Meiyazhagan, Gowri</au><au>Devaraj, Muthu</au><au>Kandasamy, Sangeetha</au><au>Manogaran, Prasath</au><au>Suresh Kumar, Govindan</au><au>Raji, Govindan</au><au>Kattimani, Vivekanand S</au><au>Easwaradas Kreedapathy, Girija</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repurposing the Antibacterial Activity of EtoposideA Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite</atitle><jtitle>ACS biomaterials science & engineering</jtitle><addtitle>ACS Biomater. 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Biofilm formation test on pristine and etoposide-loaded samples showed the inhibition of biofilm formation on etoposide loading, which was further studied by confocal laser scanning microscopy (CLSM) and colony forming units (CFUs). It has been found that etoposide-loaded HA exhibited a sustained release of the drug upto 168 h. Analysis of the inhibition mechanism of etoposide against S. aureus revealed damage to the cell membrane and has been quantified using flow cytometry by the uptake of propidium iodide. Etoposide-loaded eggshell-derived HA (EHA-ET) exhibited excellent bioactivity and cytocompatibility against mouse fibroblast cells (L929) and supressed the growth of osteosarcoma cells (MG-63). 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| subjects | Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Bio-interactions and Biocompatibility Drug Repositioning Durapatite - chemistry Durapatite - pharmacology Egg Shell Mice Staphylococcus aureus |
| title | Repurposing the Antibacterial Activity of EtoposideA Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite |
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