Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis
Purpose S. stercoralis diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this stu...
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| Veröffentlicht in: | Acta parasitologica 2022-06, Vol.67 (2), p.732-739 |
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| creator | Oliveira, Cíntia de Lima de Souza, Joelma Nascimento Souza, Alex Bruno da Silva Barreto, Nilo Manoel Pereira Vieira Ribeiro, Irlana Dias Sampaio, Larissa Mota Araújo, Weslei Almeida Costa dos Santos, Juliane Silva Batista Teixeira, Márcia Cristina Aquino Soares, Neci Matos |
| description | Purpose
S. stercoralis
diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this study was to evaluate the sensitivity of TF-Test® and Coproplus®, compared with APC, BM and Spontaneous Sedimentation (SS) methods.
Methods
One-hundred and forty male alcoholic patients, who provided three stools samples collected on alternate days, were included in this study. The most frequently found parasite was
S. stercoralis
, 20% (28/140), and the most sensitive method was APC, 96.4% (27/28), followed by BM, 89.3% (25/28) and SS, 57.1% (16/28). TF-Test® and Coproplus® presented a sensitivity of 46.4 (13/28) and 39.3% (11/28), respectively. In samples with a parasitic load of 1–10 larvae/g of feces, which occurred in 39.3% (11/28) of the infected patients, both the TF-Test® and Coproplus® methods demonstrated sensitivities of 18.2% (2/11), while APC and BM methods reached a sensitivity of 100% (11/11) (
p
|
| doi_str_mv | 10.1007/s11686-021-00484-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2621659601</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2621659601</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-9e3b87ac2e8ca5b2e0d1fdfe613a9e5e73d921c8b13fbd0b4ab9934cb09f71373</originalsourceid><addsrcrecordid>eNp9kU1uFDEQhS1ERELgAiwiS2zYmPin2-1eoskPIyViwbC23O7ypKMee-LqjjSX4hCcLE4mBIlFVi7LX716rkfIJ8G_Cs6bUxRCG824FIzzylRMvSFHwrSaCVOLt6WWijNppDgk7xFvC6SNMe_IoaoLL011RGAZIQTw03APERBpCnR1wVaA05_f1MWeLtI2p-04Y7lfw3STeqRDpD-nnOJ6N6ahB6Q4QfYpu3FAuoxPeinSs8GtY8IBP5CD4EaEj8_nMfl1cb5afGdXPy6Xi29XzCupJ9aC6kzjvATjXd1J4L0IfQAtlGuhhkb1rRTedEKFrudd5bq2VZXveBsaoRp1TL7sdYvlu7n8wW4G9DCOLkKa0Uotha5bzUVBP_-H3qY5x-KuUNooaer6kZJ7yueEmCHYbR42Lu-s4PYxBLsPwZYQ7FMIVpWmk2fpudtA_9Lyd-sFUHsAy1NcQ_43-xXZB4uglLs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2668328551</pqid></control><display><type>article</type><title>Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis</title><source>Springer Nature - Complete Springer Journals</source><creator>Oliveira, Cíntia de Lima ; de Souza, Joelma Nascimento ; Souza, Alex Bruno da Silva ; Barreto, Nilo Manoel Pereira Vieira ; Ribeiro, Irlana Dias ; Sampaio, Larissa Mota ; Araújo, Weslei Almeida Costa ; dos Santos, Juliane Silva Batista ; Teixeira, Márcia Cristina Aquino ; Soares, Neci Matos</creator><creatorcontrib>Oliveira, Cíntia de Lima ; de Souza, Joelma Nascimento ; Souza, Alex Bruno da Silva ; Barreto, Nilo Manoel Pereira Vieira ; Ribeiro, Irlana Dias ; Sampaio, Larissa Mota ; Araújo, Weslei Almeida Costa ; dos Santos, Juliane Silva Batista ; Teixeira, Márcia Cristina Aquino ; Soares, Neci Matos</creatorcontrib><description>Purpose
S. stercoralis
diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this study was to evaluate the sensitivity of TF-Test® and Coproplus®, compared with APC, BM and Spontaneous Sedimentation (SS) methods.
Methods
One-hundred and forty male alcoholic patients, who provided three stools samples collected on alternate days, were included in this study. The most frequently found parasite was
S. stercoralis
, 20% (28/140), and the most sensitive method was APC, 96.4% (27/28), followed by BM, 89.3% (25/28) and SS, 57.1% (16/28). TF-Test® and Coproplus® presented a sensitivity of 46.4 (13/28) and 39.3% (11/28), respectively. In samples with a parasitic load of 1–10 larvae/g of feces, which occurred in 39.3% (11/28) of the infected patients, both the TF-Test® and Coproplus® methods demonstrated sensitivities of 18.2% (2/11), while APC and BM methods reached a sensitivity of 100% (11/11) (
p
< 0.05). For other intestinal helminth infections, TF-Test® and Coproplus® sensitivities were 22.2 (4/18) and 11.1% (2/18), respectively, this being lower than the SS, 66.7% (12/18) (
p
< 0.05). On the other hand, for protozoa infection diagnosis, TF-Test® and Coproplus® presented the highest sensitivities, 62.2 (46/74) and 43.2% (32/74), respectively.
Conclusion
TF-Test® and Coproplus® methods presented the lowest sensitivities for
S. stercoralis
and other helminth infection diagnosis; therefore, they can be indicated for use in parasitological diagnosis, only when associated with other more effective methods of helminth identification.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1007/s11686-021-00484-3</identifier><identifier>PMID: 35048284</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Systematics/Taxonomy/Biogeography ; Biomedical and Life Sciences ; Biomedicine ; Diagnosis ; Ecology ; Feces ; Infections ; Larvae ; Medical Microbiology ; Microbiology ; Original Paper ; Parasites ; Parasitic diseases ; Parasitology ; Protozoa ; Sensitivity analysis</subject><ispartof>Acta parasitologica, 2022-06, Vol.67 (2), p.732-739</ispartof><rights>The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2021</rights><rights>2021. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.</rights><rights>The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-9e3b87ac2e8ca5b2e0d1fdfe613a9e5e73d921c8b13fbd0b4ab9934cb09f71373</cites><orcidid>0000-0001-5369-5561 ; 0000-0003-3459-3599 ; 0000-0003-2621-5256 ; 0000-0002-6088-4296 ; 0000-0001-6491-6702 ; 0000-0003-0477-5092 ; 0000-0002-5355-8695 ; 0000-0003-1409-9884 ; 0000-0002-3631-3223 ; 0000-0002-1397-1362</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11686-021-00484-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11686-021-00484-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35048284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira, Cíntia de Lima</creatorcontrib><creatorcontrib>de Souza, Joelma Nascimento</creatorcontrib><creatorcontrib>Souza, Alex Bruno da Silva</creatorcontrib><creatorcontrib>Barreto, Nilo Manoel Pereira Vieira</creatorcontrib><creatorcontrib>Ribeiro, Irlana Dias</creatorcontrib><creatorcontrib>Sampaio, Larissa Mota</creatorcontrib><creatorcontrib>Araújo, Weslei Almeida Costa</creatorcontrib><creatorcontrib>dos Santos, Juliane Silva Batista</creatorcontrib><creatorcontrib>Teixeira, Márcia Cristina Aquino</creatorcontrib><creatorcontrib>Soares, Neci Matos</creatorcontrib><title>Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>Purpose
S. stercoralis
diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this study was to evaluate the sensitivity of TF-Test® and Coproplus®, compared with APC, BM and Spontaneous Sedimentation (SS) methods.
Methods
One-hundred and forty male alcoholic patients, who provided three stools samples collected on alternate days, were included in this study. The most frequently found parasite was
S. stercoralis
, 20% (28/140), and the most sensitive method was APC, 96.4% (27/28), followed by BM, 89.3% (25/28) and SS, 57.1% (16/28). TF-Test® and Coproplus® presented a sensitivity of 46.4 (13/28) and 39.3% (11/28), respectively. In samples with a parasitic load of 1–10 larvae/g of feces, which occurred in 39.3% (11/28) of the infected patients, both the TF-Test® and Coproplus® methods demonstrated sensitivities of 18.2% (2/11), while APC and BM methods reached a sensitivity of 100% (11/11) (
p
< 0.05). For other intestinal helminth infections, TF-Test® and Coproplus® sensitivities were 22.2 (4/18) and 11.1% (2/18), respectively, this being lower than the SS, 66.7% (12/18) (
p
< 0.05). On the other hand, for protozoa infection diagnosis, TF-Test® and Coproplus® presented the highest sensitivities, 62.2 (46/74) and 43.2% (32/74), respectively.
Conclusion
TF-Test® and Coproplus® methods presented the lowest sensitivities for
S. stercoralis
and other helminth infection diagnosis; therefore, they can be indicated for use in parasitological diagnosis, only when associated with other more effective methods of helminth identification.</description><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Diagnosis</subject><subject>Ecology</subject><subject>Feces</subject><subject>Infections</subject><subject>Larvae</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Protozoa</subject><subject>Sensitivity analysis</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kU1uFDEQhS1ERELgAiwiS2zYmPin2-1eoskPIyViwbC23O7ypKMee-LqjjSX4hCcLE4mBIlFVi7LX716rkfIJ8G_Cs6bUxRCG824FIzzylRMvSFHwrSaCVOLt6WWijNppDgk7xFvC6SNMe_IoaoLL011RGAZIQTw03APERBpCnR1wVaA05_f1MWeLtI2p-04Y7lfw3STeqRDpD-nnOJ6N6ahB6Q4QfYpu3FAuoxPeinSs8GtY8IBP5CD4EaEj8_nMfl1cb5afGdXPy6Xi29XzCupJ9aC6kzjvATjXd1J4L0IfQAtlGuhhkb1rRTedEKFrudd5bq2VZXveBsaoRp1TL7sdYvlu7n8wW4G9DCOLkKa0Uotha5bzUVBP_-H3qY5x-KuUNooaer6kZJ7yueEmCHYbR42Lu-s4PYxBLsPwZYQ7FMIVpWmk2fpudtA_9Lyd-sFUHsAy1NcQ_43-xXZB4uglLs</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Oliveira, Cíntia de Lima</creator><creator>de Souza, Joelma Nascimento</creator><creator>Souza, Alex Bruno da Silva</creator><creator>Barreto, Nilo Manoel Pereira Vieira</creator><creator>Ribeiro, Irlana Dias</creator><creator>Sampaio, Larissa Mota</creator><creator>Araújo, Weslei Almeida Costa</creator><creator>dos Santos, Juliane Silva Batista</creator><creator>Teixeira, Márcia Cristina Aquino</creator><creator>Soares, Neci Matos</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5369-5561</orcidid><orcidid>https://orcid.org/0000-0003-3459-3599</orcidid><orcidid>https://orcid.org/0000-0003-2621-5256</orcidid><orcidid>https://orcid.org/0000-0002-6088-4296</orcidid><orcidid>https://orcid.org/0000-0001-6491-6702</orcidid><orcidid>https://orcid.org/0000-0003-0477-5092</orcidid><orcidid>https://orcid.org/0000-0002-5355-8695</orcidid><orcidid>https://orcid.org/0000-0003-1409-9884</orcidid><orcidid>https://orcid.org/0000-0002-3631-3223</orcidid><orcidid>https://orcid.org/0000-0002-1397-1362</orcidid></search><sort><creationdate>20220601</creationdate><title>Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis</title><author>Oliveira, Cíntia de Lima ; de Souza, Joelma Nascimento ; Souza, Alex Bruno da Silva ; Barreto, Nilo Manoel Pereira Vieira ; Ribeiro, Irlana Dias ; Sampaio, Larissa Mota ; Araújo, Weslei Almeida Costa ; dos Santos, Juliane Silva Batista ; Teixeira, Márcia Cristina Aquino ; Soares, Neci Matos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-9e3b87ac2e8ca5b2e0d1fdfe613a9e5e73d921c8b13fbd0b4ab9934cb09f71373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Diagnosis</topic><topic>Ecology</topic><topic>Feces</topic><topic>Infections</topic><topic>Larvae</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Parasitology</topic><topic>Protozoa</topic><topic>Sensitivity analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Cíntia de Lima</creatorcontrib><creatorcontrib>de Souza, Joelma Nascimento</creatorcontrib><creatorcontrib>Souza, Alex Bruno da Silva</creatorcontrib><creatorcontrib>Barreto, Nilo Manoel Pereira Vieira</creatorcontrib><creatorcontrib>Ribeiro, Irlana Dias</creatorcontrib><creatorcontrib>Sampaio, Larissa Mota</creatorcontrib><creatorcontrib>Araújo, Weslei Almeida Costa</creatorcontrib><creatorcontrib>dos Santos, Juliane Silva Batista</creatorcontrib><creatorcontrib>Teixeira, Márcia Cristina Aquino</creatorcontrib><creatorcontrib>Soares, Neci Matos</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Cíntia de Lima</au><au>de Souza, Joelma Nascimento</au><au>Souza, Alex Bruno da Silva</au><au>Barreto, Nilo Manoel Pereira Vieira</au><au>Ribeiro, Irlana Dias</au><au>Sampaio, Larissa Mota</au><au>Araújo, Weslei Almeida Costa</au><au>dos Santos, Juliane Silva Batista</au><au>Teixeira, Márcia Cristina Aquino</au><au>Soares, Neci Matos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>67</volume><issue>2</issue><spage>732</spage><epage>739</epage><pages>732-739</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Purpose
S. stercoralis
diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this study was to evaluate the sensitivity of TF-Test® and Coproplus®, compared with APC, BM and Spontaneous Sedimentation (SS) methods.
Methods
One-hundred and forty male alcoholic patients, who provided three stools samples collected on alternate days, were included in this study. The most frequently found parasite was
S. stercoralis
, 20% (28/140), and the most sensitive method was APC, 96.4% (27/28), followed by BM, 89.3% (25/28) and SS, 57.1% (16/28). TF-Test® and Coproplus® presented a sensitivity of 46.4 (13/28) and 39.3% (11/28), respectively. In samples with a parasitic load of 1–10 larvae/g of feces, which occurred in 39.3% (11/28) of the infected patients, both the TF-Test® and Coproplus® methods demonstrated sensitivities of 18.2% (2/11), while APC and BM methods reached a sensitivity of 100% (11/11) (
p
< 0.05). For other intestinal helminth infections, TF-Test® and Coproplus® sensitivities were 22.2 (4/18) and 11.1% (2/18), respectively, this being lower than the SS, 66.7% (12/18) (
p
< 0.05). On the other hand, for protozoa infection diagnosis, TF-Test® and Coproplus® presented the highest sensitivities, 62.2 (46/74) and 43.2% (32/74), respectively.
Conclusion
TF-Test® and Coproplus® methods presented the lowest sensitivities for
S. stercoralis
and other helminth infection diagnosis; therefore, they can be indicated for use in parasitological diagnosis, only when associated with other more effective methods of helminth identification.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>35048284</pmid><doi>10.1007/s11686-021-00484-3</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5369-5561</orcidid><orcidid>https://orcid.org/0000-0003-3459-3599</orcidid><orcidid>https://orcid.org/0000-0003-2621-5256</orcidid><orcidid>https://orcid.org/0000-0002-6088-4296</orcidid><orcidid>https://orcid.org/0000-0001-6491-6702</orcidid><orcidid>https://orcid.org/0000-0003-0477-5092</orcidid><orcidid>https://orcid.org/0000-0002-5355-8695</orcidid><orcidid>https://orcid.org/0000-0003-1409-9884</orcidid><orcidid>https://orcid.org/0000-0002-3631-3223</orcidid><orcidid>https://orcid.org/0000-0002-1397-1362</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Acta parasitologica, 2022-06, Vol.67 (2), p.732-739 |
| issn | 1230-2821 1896-1851 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2621659601 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Systematics/Taxonomy/Biogeography Biomedical and Life Sciences Biomedicine Diagnosis Ecology Feces Infections Larvae Medical Microbiology Microbiology Original Paper Parasites Parasitic diseases Parasitology Protozoa Sensitivity analysis |
| title | Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis |
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