Topical Effects of N -Acetyl Cysteine and Doxycycline on Inflammatory and Angiogenic Factors in the Rat Model of Alkali-Burned Cornea

The aim of this study was to analyze the single and combined effects of -acetyl cysteine (NAC) and doxycycline (Dox) on the inflammatory and angiogenic factors in the rat model of alkali-burned cornea. Rats were treated with a single and combined 0.5% NAC and 12.5 μg/mL Dox eye drops and evaluated o...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of interferon & cytokine research 2022-02, Vol.42 (2), p.82-89
Hauptverfasser:	Khoshdel, Ali Reza, Emami Aleagha, Omid, Shahriary, Alireza, Aghamollaei, Hossein, Najjar Asiabani, Farshad
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcysteine Acetylcysteine - metabolism Acetylcysteine - pharmacology Alkalies - metabolism Alkalies - pharmacology Angiogenesis Angiogenesis Inducing Agents - metabolism Angiogenesis Inducing Agents - pharmacology Animals Antibiotics Antioxidants Burns, Chemical - complications Burns, Chemical - drug therapy Burns, Chemical - metabolism Catalase Combined treatment Complications Cornea Cornea - metabolism Corneal Neovascularization - etiology Corneal Neovascularization - genetics Cysteine Disease Models, Animal Doxycycline Doxycycline - metabolism Doxycycline - pharmacology Enzymatic activity Enzyme activity Enzymes Eye Burns - chemically induced Eye Burns - complications Eye Burns - drug therapy Gelatinase A Gelatinase B Gene expression Genes Glutathione Glutathione peroxidase Inflammation mRNA Peroxidase Polymerase chain reaction Rats Sodium hydroxide Sodium Hydroxide - metabolism Sodium Hydroxide - pharmacology Superoxide dismutase Superoxide Dismutase - metabolism Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α Vascular endothelial growth factor Vascularization
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The aim of this study was to analyze the single and combined effects of -acetyl cysteine (NAC) and doxycycline (Dox) on the inflammatory and angiogenic factors in the rat model of alkali-burned cornea. Rats were treated with a single and combined 0.5% NAC and 12.5 μg/mL Dox eye drops and evaluated on days 3, 7, and 28. In the corneas of various groups, the activity of Catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) enzymes was assessed. The expression of inflammatory factors ( , , and ) and angiogenic factors ( , and ) was measured using real-time polymerase chain reaction. The antioxidant enzyme activities decreased substantially 3 days after injury with sodium hydroxide (NaOH). NAC and combined NAC+ Dox topical treatments increased the SOD enzyme activity on day 28 (
ISSN:	1079-9907 1557-7465
DOI:	10.1089/jir.2021.0150