Molecularly pure miktoarm spherical nucleic acids: preparation and usage as a scaffold for abiotic intracellular catalysis
We present a fullerene-based strategy that allows the synthesis of molecularly pure miktoarm spherical nucleic acids (SNAs) with diverse structures, which, with post-functionalization, could serve as efficient scaffolds for intracellular catalysis. The SNA structure promotes cell permeability, nucle...
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| Veröffentlicht in: | Chemical science (Cambridge) 2021-12, Vol.12 (48), p.15843-15848 |
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| container_title | Chemical science (Cambridge) |
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| creator | Zhang, Bohan Bai, Silei Chao, Xiangyu Wu, Tong Chen, Zhiyong Cheng, Zehong Xiao, Yue Zhang, Ke Bai, Yugang |
| description | We present a fullerene-based strategy that allows the synthesis of molecularly pure miktoarm spherical nucleic acids (SNAs) with diverse structures, which, with post-functionalization, could serve as efficient scaffolds for intracellular catalysis. The SNA structure promotes cell permeability, nucleic acid stability, and catalytic efficiency, making the platform ideal for
in cellulo
reactions. Consequently, the tris(triazole)-bearing miktoarm SNA was able to effectively mediate intracellular copper-catalyzed alkyne-azide cycloaddition at nanomolar level of copper, and facilitate the same reaction in live zebrafish.
Biocompatible nano-constructs, with definite molecular structures and programmable subunits, can potentially be used as biocatalysts with modular functional moieties. |
| doi_str_mv | 10.1039/d1sc04833c |
| format | Article |
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in cellulo
reactions. Consequently, the tris(triazole)-bearing miktoarm SNA was able to effectively mediate intracellular copper-catalyzed alkyne-azide cycloaddition at nanomolar level of copper, and facilitate the same reaction in live zebrafish.
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in cellulo
reactions. Consequently, the tris(triazole)-bearing miktoarm SNA was able to effectively mediate intracellular copper-catalyzed alkyne-azide cycloaddition at nanomolar level of copper, and facilitate the same reaction in live zebrafish.
Biocompatible nano-constructs, with definite molecular structures and programmable subunits, can potentially be used as biocatalysts with modular functional moieties.</description><subject>Alkynes</subject><subject>Catalysis</subject><subject>Chemistry</subject><subject>Copper</subject><subject>Cycloaddition</subject><subject>Nucleic acids</subject><subject>Scaffolds</subject><subject>Zebrafish</subject><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkktv1TAQhSMEolXbDXuQJTYI6Ra_4jgskNDlUaQiFsDamkwmrYsTBztBuvz6JtxyeXjjkeab4zM6LopHgp8LruoXrcjItVUK7xXHkmuxMaWq7x9qyY-Ks5xv-HKUEqWsHhZHquRSC26Pi58fYyCcA6SwY-OciPX-2xQh9SyP15Q8QmDDjIE8MkDf5pdsTDRCgsnHgcHQsjnDFTHIDFhG6LoYWtbFxKDxcVrG_DAlQAphfYYhTBB22efT4kEHIdPZ3X1SfH339sv2YnP56f2H7evLDWppp03ZaCqN5YJTVUvTWGN402krhTJAoCXUQtS11Mq2gMhVZwiNVWQqbFpp1Unxaq87zk1PLdJqJ7gx-R7SzkXw7t_O4K_dVfzhrKlkJdUi8OxOIMXvM-XJ9T6v-8BAcc5OGlGXWlZVvaBP_0Nv4pyGZb2VWgOQFV-o53sKU8w5UXcwI7hbU3VvxOftr1S3C_zkb_sH9HeGC_B4D6SMh-6fb6FuAcrTqJo</recordid><startdate>20211215</startdate><enddate>20211215</enddate><creator>Zhang, Bohan</creator><creator>Bai, Silei</creator><creator>Chao, Xiangyu</creator><creator>Wu, Tong</creator><creator>Chen, Zhiyong</creator><creator>Cheng, Zehong</creator><creator>Xiao, Yue</creator><creator>Zhang, Ke</creator><creator>Bai, Yugang</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9834-7517</orcidid></search><sort><creationdate>20211215</creationdate><title>Molecularly pure miktoarm spherical nucleic acids: preparation and usage as a scaffold for abiotic intracellular catalysis</title><author>Zhang, Bohan ; Bai, Silei ; Chao, Xiangyu ; Wu, Tong ; Chen, Zhiyong ; Cheng, Zehong ; Xiao, Yue ; Zhang, Ke ; Bai, Yugang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-5b4e568010e7926b8660bf482136aea42a911992438dacc03f6ec683e67cbd283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alkynes</topic><topic>Catalysis</topic><topic>Chemistry</topic><topic>Copper</topic><topic>Cycloaddition</topic><topic>Nucleic acids</topic><topic>Scaffolds</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Bohan</creatorcontrib><creatorcontrib>Bai, Silei</creatorcontrib><creatorcontrib>Chao, Xiangyu</creatorcontrib><creatorcontrib>Wu, Tong</creatorcontrib><creatorcontrib>Chen, Zhiyong</creatorcontrib><creatorcontrib>Cheng, Zehong</creatorcontrib><creatorcontrib>Xiao, Yue</creatorcontrib><creatorcontrib>Zhang, Ke</creatorcontrib><creatorcontrib>Bai, Yugang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Bohan</au><au>Bai, Silei</au><au>Chao, Xiangyu</au><au>Wu, Tong</au><au>Chen, Zhiyong</au><au>Cheng, Zehong</au><au>Xiao, Yue</au><au>Zhang, Ke</au><au>Bai, Yugang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecularly pure miktoarm spherical nucleic acids: preparation and usage as a scaffold for abiotic intracellular catalysis</atitle><jtitle>Chemical science (Cambridge)</jtitle><addtitle>Chem Sci</addtitle><date>2021-12-15</date><risdate>2021</risdate><volume>12</volume><issue>48</issue><spage>15843</spage><epage>15848</epage><pages>15843-15848</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>We present a fullerene-based strategy that allows the synthesis of molecularly pure miktoarm spherical nucleic acids (SNAs) with diverse structures, which, with post-functionalization, could serve as efficient scaffolds for intracellular catalysis. The SNA structure promotes cell permeability, nucleic acid stability, and catalytic efficiency, making the platform ideal for
in cellulo
reactions. Consequently, the tris(triazole)-bearing miktoarm SNA was able to effectively mediate intracellular copper-catalyzed alkyne-azide cycloaddition at nanomolar level of copper, and facilitate the same reaction in live zebrafish.
Biocompatible nano-constructs, with definite molecular structures and programmable subunits, can potentially be used as biocatalysts with modular functional moieties.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35024108</pmid><doi>10.1039/d1sc04833c</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-9834-7517</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Alkynes Catalysis Chemistry Copper Cycloaddition Nucleic acids Scaffolds Zebrafish |
| title | Molecularly pure miktoarm spherical nucleic acids: preparation and usage as a scaffold for abiotic intracellular catalysis |
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