Immune tumoral microenvironment in gliomas: focus on CD3+ T cells, Vδ1+ T cells, and microglia/macrophages
Gliomas are histologically defined as low-grade gliomas (LGG) and high-grade gliomas (HGG). The most common type of HGG is the glioblastoma (GBM). We aimed to determine the immunological characteristics of CD3 T-cells, Vδ1 T-cells, and microglia/macrophages infiltrating brain gliomas. We collected 2...
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| Veröffentlicht in: | Immunologic research 2022-04, Vol.70 (2), p.224-239 |
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| creator | Belghali, Moulay Yassine Admou, Brahim Brahimi, Maroua Khouchani, Mouna Ba-M’hamed, Saadia |
| description | Gliomas are histologically defined as low-grade gliomas (LGG) and high-grade gliomas (HGG). The most common type of HGG is the glioblastoma (GBM). We aimed to determine the immunological characteristics of CD3 T-cells, Vδ1 T-cells, and microglia/macrophages infiltrating brain gliomas. We collected 24 formalin-fixed paraffin-embedded samples issued from 19 cases of GBM and 5 cases of LGG. An immunohistochemical analysis was performed using anti-CD3, anti-Vδ1, and anti-iba-1 antibodies. Labelling indexes (LI) of CD3 and Vδ1 were evaluated quantitatively, and other CD3, Vδ1, and iba-1 staining characteristics were evaluated qualitatively. The median age of patients was 49 years in GBM and 52 years in LGG. The sex ratio was 1.4 and GBM predominated in males (
p
= 0.05). In GBM, the medians of CD3-LI and Vδ1-LI were 30 and 3.5 respectively. CD3-LI inversely correlated with survival in GBM cases (
r
= − 0.543;
p
= 0.016). CD3 staining intensity correlated with CD3-LI (
p
|
| doi_str_mv | 10.1007/s12026-022-09260-5 |
| format | Article |
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p
= 0.05). In GBM, the medians of CD3-LI and Vδ1-LI were 30 and 3.5 respectively. CD3-LI inversely correlated with survival in GBM cases (
r
= − 0.543;
p
= 0.016). CD3 staining intensity correlated with CD3-LI (
p
< 0.0001) and with the survival in GBM cases (
p
= 0.003). Compared to LGG, the CD3-LI, the intensity of intra-tumoral Vδ1 staining, and the amount of iba-1 were higher in GBM (
p
= 0.042;
p
= 0.014; and
p
= 0.001 respectively). The iba-1 organization was more amoeboid in older patients and more branched in younger patients (
p
= 0.028) and tended to be more amoeboid in cases with high iba-1 amount (
p
= 0.09). Our results suggest that a high level of CD3-LI and a strong intra-tumoral infiltration of Vδ1 T-cells as well as a high involvement of TAM can be considered potential markers of poor prognosis of GBM. However, this requires further studies on more balanced GBM-LGG sample, including an expanded panel of biomarkers.</description><identifier>ISSN: 0257-277X</identifier><identifier>EISSN: 1559-0755</identifier><identifier>DOI: 10.1007/s12026-022-09260-5</identifier><identifier>PMID: 35006549</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aged ; Allergology ; Antibodies ; Biomarkers ; Biomedical and Life Sciences ; Biomedicine ; Brain Neoplasms ; CD3 antigen ; Glioblastoma ; Glioblastoma - pathology ; Glioma ; Humans ; Immunology ; Internal Medicine ; Labeling ; Lymphocytes ; Lymphocytes T ; Macrophages ; Macrophages - pathology ; Male ; Medical prognosis ; Medicine/Public Health ; Metastases ; Microenvironments ; Microglia ; Microglia - pathology ; Middle Aged ; Original Article ; Paraffin ; Paraffins ; Sex ratio ; Staining ; Survival ; T-Lymphocytes - pathology ; Tumor Microenvironment</subject><ispartof>Immunologic research, 2022-04, Vol.70 (2), p.224-239</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f2fedaac6db94cab4c793647488abb123d7cace17555bf6de81114de34f5106f3</citedby><cites>FETCH-LOGICAL-c375t-f2fedaac6db94cab4c793647488abb123d7cace17555bf6de81114de34f5106f3</cites><orcidid>0000-0003-4098-8992</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12026-022-09260-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12026-022-09260-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35006549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belghali, Moulay Yassine</creatorcontrib><creatorcontrib>Admou, Brahim</creatorcontrib><creatorcontrib>Brahimi, Maroua</creatorcontrib><creatorcontrib>Khouchani, Mouna</creatorcontrib><creatorcontrib>Ba-M’hamed, Saadia</creatorcontrib><title>Immune tumoral microenvironment in gliomas: focus on CD3+ T cells, Vδ1+ T cells, and microglia/macrophages</title><title>Immunologic research</title><addtitle>Immunol Res</addtitle><addtitle>Immunol Res</addtitle><description>Gliomas are histologically defined as low-grade gliomas (LGG) and high-grade gliomas (HGG). The most common type of HGG is the glioblastoma (GBM). We aimed to determine the immunological characteristics of CD3 T-cells, Vδ1 T-cells, and microglia/macrophages infiltrating brain gliomas. We collected 24 formalin-fixed paraffin-embedded samples issued from 19 cases of GBM and 5 cases of LGG. An immunohistochemical analysis was performed using anti-CD3, anti-Vδ1, and anti-iba-1 antibodies. Labelling indexes (LI) of CD3 and Vδ1 were evaluated quantitatively, and other CD3, Vδ1, and iba-1 staining characteristics were evaluated qualitatively. The median age of patients was 49 years in GBM and 52 years in LGG. The sex ratio was 1.4 and GBM predominated in males (
p
= 0.05). In GBM, the medians of CD3-LI and Vδ1-LI were 30 and 3.5 respectively. CD3-LI inversely correlated with survival in GBM cases (
r
= − 0.543;
p
= 0.016). CD3 staining intensity correlated with CD3-LI (
p
< 0.0001) and with the survival in GBM cases (
p
= 0.003). Compared to LGG, the CD3-LI, the intensity of intra-tumoral Vδ1 staining, and the amount of iba-1 were higher in GBM (
p
= 0.042;
p
= 0.014; and
p
= 0.001 respectively). The iba-1 organization was more amoeboid in older patients and more branched in younger patients (
p
= 0.028) and tended to be more amoeboid in cases with high iba-1 amount (
p
= 0.09). Our results suggest that a high level of CD3-LI and a strong intra-tumoral infiltration of Vδ1 T-cells as well as a high involvement of TAM can be considered potential markers of poor prognosis of GBM. However, this requires further studies on more balanced GBM-LGG sample, including an expanded panel of biomarkers.</description><subject>Aged</subject><subject>Allergology</subject><subject>Antibodies</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain Neoplasms</subject><subject>CD3 antigen</subject><subject>Glioblastoma</subject><subject>Glioblastoma - pathology</subject><subject>Glioma</subject><subject>Humans</subject><subject>Immunology</subject><subject>Internal Medicine</subject><subject>Labeling</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Macrophages - pathology</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medicine/Public Health</subject><subject>Metastases</subject><subject>Microenvironments</subject><subject>Microglia</subject><subject>Microglia - pathology</subject><subject>Middle Aged</subject><subject>Original Article</subject><subject>Paraffin</subject><subject>Paraffins</subject><subject>Sex ratio</subject><subject>Staining</subject><subject>Survival</subject><subject>T-Lymphocytes - pathology</subject><subject>Tumor Microenvironment</subject><issn>0257-277X</issn><issn>1559-0755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kctO3DAUhi0EgumUF2CBLLFBKun47qQ7NIWChNTNtGJnOY4zzRDbU3tSiffiOXgmPIRC1QUrH-t85z-XH4AjjD5jhOQsYYKIKBAhBaqIQAXfARPMeVUgyfkumCDCZUGkvD0AH1JaIYQFY3QfHFCOkOCsmoC7a-cGb-FmcCHqHrrOxGD9ny4G76zfwM7DZd8Fp9MX2AYzJBg8nH-ln-ACGtv36Qz-fHzA_3y1b0aZXKdnTudo_UsvbfoI9lrdJ3v48k7Bj8uLxfyquPn-7Xp-flMYKvmmaElrG62NaOqKGV0zIysqmGRlqesaE9pIo43FeUdet6KxJcaYNZaylmMkWjoFp6PuOobfg00b5bq0HU57G4akiMAlx4wJntGT_9BVGKLP02WKlrkHZjJTZKTyKilF26p17JyO9wojtbVCjVaobIV6tkJtpY9fpIfa2ea15O_tM0BHIOWUX9r41vsd2Se12pQk</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Belghali, Moulay Yassine</creator><creator>Admou, Brahim</creator><creator>Brahimi, Maroua</creator><creator>Khouchani, Mouna</creator><creator>Ba-M’hamed, Saadia</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4098-8992</orcidid></search><sort><creationdate>20220401</creationdate><title>Immune tumoral microenvironment in gliomas: focus on CD3+ T cells, Vδ1+ T cells, and microglia/macrophages</title><author>Belghali, Moulay Yassine ; 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The most common type of HGG is the glioblastoma (GBM). We aimed to determine the immunological characteristics of CD3 T-cells, Vδ1 T-cells, and microglia/macrophages infiltrating brain gliomas. We collected 24 formalin-fixed paraffin-embedded samples issued from 19 cases of GBM and 5 cases of LGG. An immunohistochemical analysis was performed using anti-CD3, anti-Vδ1, and anti-iba-1 antibodies. Labelling indexes (LI) of CD3 and Vδ1 were evaluated quantitatively, and other CD3, Vδ1, and iba-1 staining characteristics were evaluated qualitatively. The median age of patients was 49 years in GBM and 52 years in LGG. The sex ratio was 1.4 and GBM predominated in males (
p
= 0.05). In GBM, the medians of CD3-LI and Vδ1-LI were 30 and 3.5 respectively. CD3-LI inversely correlated with survival in GBM cases (
r
= − 0.543;
p
= 0.016). CD3 staining intensity correlated with CD3-LI (
p
< 0.0001) and with the survival in GBM cases (
p
= 0.003). Compared to LGG, the CD3-LI, the intensity of intra-tumoral Vδ1 staining, and the amount of iba-1 were higher in GBM (
p
= 0.042;
p
= 0.014; and
p
= 0.001 respectively). The iba-1 organization was more amoeboid in older patients and more branched in younger patients (
p
= 0.028) and tended to be more amoeboid in cases with high iba-1 amount (
p
= 0.09). Our results suggest that a high level of CD3-LI and a strong intra-tumoral infiltration of Vδ1 T-cells as well as a high involvement of TAM can be considered potential markers of poor prognosis of GBM. However, this requires further studies on more balanced GBM-LGG sample, including an expanded panel of biomarkers.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35006549</pmid><doi>10.1007/s12026-022-09260-5</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0003-4098-8992</orcidid></addata></record> |
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| subjects | Aged Allergology Antibodies Biomarkers Biomedical and Life Sciences Biomedicine Brain Neoplasms CD3 antigen Glioblastoma Glioblastoma - pathology Glioma Humans Immunology Internal Medicine Labeling Lymphocytes Lymphocytes T Macrophages Macrophages - pathology Male Medical prognosis Medicine/Public Health Metastases Microenvironments Microglia Microglia - pathology Middle Aged Original Article Paraffin Paraffins Sex ratio Staining Survival T-Lymphocytes - pathology Tumor Microenvironment |
| title | Immune tumoral microenvironment in gliomas: focus on CD3+ T cells, Vδ1+ T cells, and microglia/macrophages |
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