Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS2 and AuNPs coupling with HRP enzyme signal amplification
A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated 3D-flower-like MoS 2 (3D MoS 2 -rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles (S1-AuN...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2022-01, Vol.189 (1), p.49-49, Article 49 |
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| container_title | Mikrochimica acta (1966) |
| container_volume | 189 |
| creator | Dong, Jiangbo Yang, Huisi Zhao, Jiaying Wen, Li He, Congjuan Hu, Zhikun Li, Jiawei Huo, Danqun Hou, Changjun |
| description | A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated 3D-flower-like MoS
2
(3D MoS
2
-rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles (S1-AuNPs-HRP) as signal amplification probes. Herein, 3D MoS
2
-rGO nanocomposites not only had a large specific surface area and excellent conductivity, but also provided more attachment sites for electrodepositing AuNPs. In the presence of target miRNA, a sandwich structure was formed, and the determination of the miRNA-21 was carried out by measuring the DPV response of H
2
O
2
mediated by hydroquinone (HQ) at a potential of + 0.052 V (vs AgCl reference electrode). Under the optimal experimental conditions, the as-prepared biosensor enabled the ultrasensitive detection of miRNA-21 from 5 fM to 0.5 μM with the low detection limit of 0.54 fM (
S
/
N
= 3), comparable or lower than previous reported methods for miRNA-21 detection, which benefited from the synergistic amplification of 3D MoS
2
-rGO and AuNPs-HRP. The prepared biosensor showed satisfactory selectivity, reproducibility, and stability towards miRNA-21 detection. The biosensor was feasible for accurate and quantitative detection of miRNA-21 in normal human serum samples with RSD below 5.86%, which showed a great potential in clinical analysis and disease diagnosis.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-021-05141-0 |
| format | Article |
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2
(3D MoS
2
-rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles (S1-AuNPs-HRP) as signal amplification probes. Herein, 3D MoS
2
-rGO nanocomposites not only had a large specific surface area and excellent conductivity, but also provided more attachment sites for electrodepositing AuNPs. In the presence of target miRNA, a sandwich structure was formed, and the determination of the miRNA-21 was carried out by measuring the DPV response of H
2
O
2
mediated by hydroquinone (HQ) at a potential of + 0.052 V (vs AgCl reference electrode). Under the optimal experimental conditions, the as-prepared biosensor enabled the ultrasensitive detection of miRNA-21 from 5 fM to 0.5 μM with the low detection limit of 0.54 fM (
S
/
N
= 3), comparable or lower than previous reported methods for miRNA-21 detection, which benefited from the synergistic amplification of 3D MoS
2
-rGO and AuNPs-HRP. The prepared biosensor showed satisfactory selectivity, reproducibility, and stability towards miRNA-21 detection. The biosensor was feasible for accurate and quantitative detection of miRNA-21 in normal human serum samples with RSD below 5.86%, which showed a great potential in clinical analysis and disease diagnosis.
Graphical abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-021-05141-0</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Amplification ; Analytical Chemistry ; Biosensors ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Graphene ; Hydrogen peroxide ; Hydroquinone ; Microengineering ; MicroRNAs ; Molybdenum disulfide ; Nanochemistry ; Nanocomposites ; Nanoparticles ; Nanotechnology ; Original Paper ; Peroxidase ; Ribonucleic acid ; RNA ; Sandwich structures ; Selectivity ; Silver chloride ; Substrates ; Three dimensional flow</subject><ispartof>Mikrochimica acta (1966), 2022-01, Vol.189 (1), p.49-49, Article 49</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-d9ab169da30903dd0b920cbd7e2c216d69c2e65f07c710b46f0dbc08c4dae20b3</citedby><cites>FETCH-LOGICAL-c352t-d9ab169da30903dd0b920cbd7e2c216d69c2e65f07c710b46f0dbc08c4dae20b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-021-05141-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-021-05141-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Dong, Jiangbo</creatorcontrib><creatorcontrib>Yang, Huisi</creatorcontrib><creatorcontrib>Zhao, Jiaying</creatorcontrib><creatorcontrib>Wen, Li</creatorcontrib><creatorcontrib>He, Congjuan</creatorcontrib><creatorcontrib>Hu, Zhikun</creatorcontrib><creatorcontrib>Li, Jiawei</creatorcontrib><creatorcontrib>Huo, Danqun</creatorcontrib><creatorcontrib>Hou, Changjun</creatorcontrib><title>Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS2 and AuNPs coupling with HRP enzyme signal amplification</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated 3D-flower-like MoS
2
(3D MoS
2
-rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles (S1-AuNPs-HRP) as signal amplification probes. Herein, 3D MoS
2
-rGO nanocomposites not only had a large specific surface area and excellent conductivity, but also provided more attachment sites for electrodepositing AuNPs. In the presence of target miRNA, a sandwich structure was formed, and the determination of the miRNA-21 was carried out by measuring the DPV response of H
2
O
2
mediated by hydroquinone (HQ) at a potential of + 0.052 V (vs AgCl reference electrode). Under the optimal experimental conditions, the as-prepared biosensor enabled the ultrasensitive detection of miRNA-21 from 5 fM to 0.5 μM with the low detection limit of 0.54 fM (
S
/
N
= 3), comparable or lower than previous reported methods for miRNA-21 detection, which benefited from the synergistic amplification of 3D MoS
2
-rGO and AuNPs-HRP. The prepared biosensor showed satisfactory selectivity, reproducibility, and stability towards miRNA-21 detection. The biosensor was feasible for accurate and quantitative detection of miRNA-21 in normal human serum samples with RSD below 5.86%, which showed a great potential in clinical analysis and disease diagnosis.
Graphical abstract</description><subject>Amplification</subject><subject>Analytical Chemistry</subject><subject>Biosensors</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Graphene</subject><subject>Hydrogen peroxide</subject><subject>Hydroquinone</subject><subject>Microengineering</subject><subject>MicroRNAs</subject><subject>Molybdenum disulfide</subject><subject>Nanochemistry</subject><subject>Nanocomposites</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Peroxidase</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sandwich structures</subject><subject>Selectivity</subject><subject>Silver chloride</subject><subject>Substrates</subject><subject>Three dimensional flow</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kU9v1DAQxSNEJZaWL8DJEhcuhrGd2OS4Kn-KVErVlrPl2JNdl8QOdqJl-RZ8YwxBQuLQy8zh_d7TaF5VPWfwigGo1xlAQk2BMwoNq8t8VG1YLSRtQInH1QaASyqk4k-qpznfAzAleb2pft6a4A7e7ul8nJCM3qZ4c7UlnY8ZQ46JdCajIzGQXTLTHgOS-N07JD7YmKaYzFxk8Zb2QzxgooP_iuRTvOWkBJPtcnWdiY3LNPiwIwc_78nFzTXB8OM4Isl-F8xAzFjk3lsz-xjOqpPeDBmf_d2n1Zf37-7OL-jl5w8fz7eX1IqGz9S1pmOydUZAC8I56FoOtnMKueVMOtlajrLpQVnFoKtlD66z8MbWziCHTpxWL9fcKcVvC-ZZjz5bHAYTMC5Zc8kUV20DvKAv_kPv45LK5SslWNOALBRfqfLCnBP2ekp-NOmoGejfLem1JV1a0n9a0lBMYjXlAocdpn_RD7h-AVjKllA</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Dong, Jiangbo</creator><creator>Yang, Huisi</creator><creator>Zhao, Jiaying</creator><creator>Wen, Li</creator><creator>He, Congjuan</creator><creator>Hu, Zhikun</creator><creator>Li, Jiawei</creator><creator>Huo, Danqun</creator><creator>Hou, Changjun</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20220101</creationdate><title>Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS2 and AuNPs coupling with HRP enzyme signal amplification</title><author>Dong, Jiangbo ; Yang, Huisi ; Zhao, Jiaying ; Wen, Li ; He, Congjuan ; Hu, Zhikun ; Li, Jiawei ; Huo, Danqun ; Hou, Changjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-d9ab169da30903dd0b920cbd7e2c216d69c2e65f07c710b46f0dbc08c4dae20b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amplification</topic><topic>Analytical Chemistry</topic><topic>Biosensors</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Graphene</topic><topic>Hydrogen peroxide</topic><topic>Hydroquinone</topic><topic>Microengineering</topic><topic>MicroRNAs</topic><topic>Molybdenum disulfide</topic><topic>Nanochemistry</topic><topic>Nanocomposites</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Peroxidase</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Sandwich structures</topic><topic>Selectivity</topic><topic>Silver chloride</topic><topic>Substrates</topic><topic>Three dimensional flow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Jiangbo</creatorcontrib><creatorcontrib>Yang, Huisi</creatorcontrib><creatorcontrib>Zhao, Jiaying</creatorcontrib><creatorcontrib>Wen, Li</creatorcontrib><creatorcontrib>He, Congjuan</creatorcontrib><creatorcontrib>Hu, Zhikun</creatorcontrib><creatorcontrib>Li, Jiawei</creatorcontrib><creatorcontrib>Huo, Danqun</creatorcontrib><creatorcontrib>Hou, Changjun</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Jiangbo</au><au>Yang, Huisi</au><au>Zhao, Jiaying</au><au>Wen, Li</au><au>He, Congjuan</au><au>Hu, Zhikun</au><au>Li, Jiawei</au><au>Huo, Danqun</au><au>Hou, Changjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS2 and AuNPs coupling with HRP enzyme signal amplification</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2022-01-01</date><risdate>2022</risdate><volume>189</volume><issue>1</issue><spage>49</spage><epage>49</epage><pages>49-49</pages><artnum>49</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated 3D-flower-like MoS
2
(3D MoS
2
-rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles (S1-AuNPs-HRP) as signal amplification probes. Herein, 3D MoS
2
-rGO nanocomposites not only had a large specific surface area and excellent conductivity, but also provided more attachment sites for electrodepositing AuNPs. In the presence of target miRNA, a sandwich structure was formed, and the determination of the miRNA-21 was carried out by measuring the DPV response of H
2
O
2
mediated by hydroquinone (HQ) at a potential of + 0.052 V (vs AgCl reference electrode). Under the optimal experimental conditions, the as-prepared biosensor enabled the ultrasensitive detection of miRNA-21 from 5 fM to 0.5 μM with the low detection limit of 0.54 fM (
S
/
N
= 3), comparable or lower than previous reported methods for miRNA-21 detection, which benefited from the synergistic amplification of 3D MoS
2
-rGO and AuNPs-HRP. The prepared biosensor showed satisfactory selectivity, reproducibility, and stability towards miRNA-21 detection. The biosensor was feasible for accurate and quantitative detection of miRNA-21 in normal human serum samples with RSD below 5.86%, which showed a great potential in clinical analysis and disease diagnosis.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-021-05141-0</doi><tpages>1</tpages></addata></record> |
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| subjects | Amplification Analytical Chemistry Biosensors Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Graphene Hydrogen peroxide Hydroquinone Microengineering MicroRNAs Molybdenum disulfide Nanochemistry Nanocomposites Nanoparticles Nanotechnology Original Paper Peroxidase Ribonucleic acid RNA Sandwich structures Selectivity Silver chloride Substrates Three dimensional flow |
| title | Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS2 and AuNPs coupling with HRP enzyme signal amplification |
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