Investigation of the expression level of long non-coding RNAs in dental follicles of impacted mandibular third molars
Objectives Dental follicle (DF) is made up of mesenchymal cells and fibers surrounding the enamel organ of a developing tooth. It has been shown that cystic and neoplastic lesions can develop from the pericoronal follicles of impacted third molars (ITMs). But the molecular transformation of DF tissu...
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| Veröffentlicht in: | Clinical oral investigations 2022-03, Vol.26 (3), p.2817-2825 |
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| creator | Ege, Bilal Koparal, Mahmut Kurt, Muhammed Yusuf Bozgeyik, Esra |
| description | Objectives
Dental follicle (DF) is made up of mesenchymal cells and fibers surrounding the enamel organ of a developing tooth. It has been shown that cystic and neoplastic lesions can develop from the pericoronal follicles of impacted third molars (ITMs). But the molecular transformation of DF tissues has not yet been uncovered and remains elusive. Accordingly, in the present study, we aimed to investigate the differential expression of lncRNA genes in DF tissues associated with asymptomatic impacted mandibular third molars (IMTMs) that do not show pathological pericoronal radiolucency in radiographic examination.
Material and methods
A total of 30 patients with unilateral mesioangular IMTMs were enrolled for the study. The expressions of lncRNA genes were determined in the DF and healthy gingival tissues obtained from study patients. For the determination of lncRNA expression levels, RNA was isolated from the obtained tissues, converted to cDNA samples, and analyzed by quantitative real-time PCR method.
Results
As a result, we found that the gene expression of MEG3 was increased about 10-fold in DF tissues compared to healthy gingival tissues (
p
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| doi_str_mv | 10.1007/s00784-021-04259-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2617278537</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2617278537</sourcerecordid><originalsourceid>FETCH-LOGICAL-c347t-baf020a8ae1c27fcd4e456d73e69f599224a83bc75a21673dca8d56bb3497c7d3</originalsourceid><addsrcrecordid>eNp9kMtO5DAQRS0EGl7zAyxQlmzM-JU4WSI0PCQE0gjWlmNXGiPHbuykRf_NfAtfNm4aZsnGvqq6daV7EDqh5JwSIn_l8rQCE0YxEazu8HoHHVDBG8ylpLsfmuGma-k-Osz5hRAqGsl_oH0uurZtOn6AVrdhBXlyCz25GKo4VNMzvP-Ft2WCnDcjDyvwm4WPYVGFGLCJ1hX55_4iVy5UFsKkfTVE753xkDdeNy61mcBWow7W9bPXqQS7VAax6HyM9gbtM_z8_I_Q09Xvx8sbfPdwfXt5cYcNF3LCvR4II7rVQA2Tg7ECRN1YyaHphrrrGBO65b2RtWa0VLNGt7Zu-r4UlEZafoTOtrnLFF_nUlSNLhvwXgeIc1asoZLJtuayWNnWalLMOcGglsmNOq0VJWrDW215q8JbffBW63J0-pk_9yPY_ydfgIuBbw25rMICknqJcwql83ex_wD24I7j</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2617278537</pqid></control><display><type>article</type><title>Investigation of the expression level of long non-coding RNAs in dental follicles of impacted mandibular third molars</title><source>MEDLINE</source><source>SpringerLink_现刊</source><creator>Ege, Bilal ; Koparal, Mahmut ; Kurt, Muhammed Yusuf ; Bozgeyik, Esra</creator><creatorcontrib>Ege, Bilal ; Koparal, Mahmut ; Kurt, Muhammed Yusuf ; Bozgeyik, Esra</creatorcontrib><description>Objectives
Dental follicle (DF) is made up of mesenchymal cells and fibers surrounding the enamel organ of a developing tooth. It has been shown that cystic and neoplastic lesions can develop from the pericoronal follicles of impacted third molars (ITMs). But the molecular transformation of DF tissues has not yet been uncovered and remains elusive. Accordingly, in the present study, we aimed to investigate the differential expression of lncRNA genes in DF tissues associated with asymptomatic impacted mandibular third molars (IMTMs) that do not show pathological pericoronal radiolucency in radiographic examination.
Material and methods
A total of 30 patients with unilateral mesioangular IMTMs were enrolled for the study. The expressions of lncRNA genes were determined in the DF and healthy gingival tissues obtained from study patients. For the determination of lncRNA expression levels, RNA was isolated from the obtained tissues, converted to cDNA samples, and analyzed by quantitative real-time PCR method.
Results
As a result, we found that the gene expression of MEG3 was increased about 10-fold in DF tissues compared to healthy gingival tissues (
p
< 0.0001). In addition, NORAD expression was found to be upregulated 4.2-fold (
p
= 0.0002) in DF tissues. Also, expression level of MALAT1 was found to be decreased 1.24-fold (
p
= 0.584) and TP73-AS1 increased 2.6-fold (
p
= 0.093) in DF tissues compared to healthy gingival tissues.
Conclusions
Consequently, present findings suggest that differentially expressed lncRNAs in DFs might be associated with the various levels of cellular events including osteogenic differentiation, DNA damage, and the transformation into odontogenic pathology.
Clinical relevance
Expression levels of MEG3 and NORAD lncRNA molecules may guide clinicians in the evaluation of asymptomatic ITM dental follicles that cannot be determined radiologically and during extraction of these teeth for prophylactic purposes.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-021-04259-y</identifier><identifier>PMID: 34988693</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Dental Sac - metabolism ; Dentistry ; Humans ; Medicine ; Molar, Third - pathology ; Original Article ; Osteogenesis ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Tooth, Impacted - diagnostic imaging ; Tooth, Impacted - genetics</subject><ispartof>Clinical oral investigations, 2022-03, Vol.26 (3), p.2817-2825</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-baf020a8ae1c27fcd4e456d73e69f599224a83bc75a21673dca8d56bb3497c7d3</citedby><cites>FETCH-LOGICAL-c347t-baf020a8ae1c27fcd4e456d73e69f599224a83bc75a21673dca8d56bb3497c7d3</cites><orcidid>0000-0003-1817-1230 ; 0000-0002-1360-276X ; 0000-0002-8726-3182 ; 0000-0002-1279-0893</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-021-04259-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-021-04259-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34988693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ege, Bilal</creatorcontrib><creatorcontrib>Koparal, Mahmut</creatorcontrib><creatorcontrib>Kurt, Muhammed Yusuf</creatorcontrib><creatorcontrib>Bozgeyik, Esra</creatorcontrib><title>Investigation of the expression level of long non-coding RNAs in dental follicles of impacted mandibular third molars</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
Dental follicle (DF) is made up of mesenchymal cells and fibers surrounding the enamel organ of a developing tooth. It has been shown that cystic and neoplastic lesions can develop from the pericoronal follicles of impacted third molars (ITMs). But the molecular transformation of DF tissues has not yet been uncovered and remains elusive. Accordingly, in the present study, we aimed to investigate the differential expression of lncRNA genes in DF tissues associated with asymptomatic impacted mandibular third molars (IMTMs) that do not show pathological pericoronal radiolucency in radiographic examination.
Material and methods
A total of 30 patients with unilateral mesioangular IMTMs were enrolled for the study. The expressions of lncRNA genes were determined in the DF and healthy gingival tissues obtained from study patients. For the determination of lncRNA expression levels, RNA was isolated from the obtained tissues, converted to cDNA samples, and analyzed by quantitative real-time PCR method.
Results
As a result, we found that the gene expression of MEG3 was increased about 10-fold in DF tissues compared to healthy gingival tissues (
p
< 0.0001). In addition, NORAD expression was found to be upregulated 4.2-fold (
p
= 0.0002) in DF tissues. Also, expression level of MALAT1 was found to be decreased 1.24-fold (
p
= 0.584) and TP73-AS1 increased 2.6-fold (
p
= 0.093) in DF tissues compared to healthy gingival tissues.
Conclusions
Consequently, present findings suggest that differentially expressed lncRNAs in DFs might be associated with the various levels of cellular events including osteogenic differentiation, DNA damage, and the transformation into odontogenic pathology.
Clinical relevance
Expression levels of MEG3 and NORAD lncRNA molecules may guide clinicians in the evaluation of asymptomatic ITM dental follicles that cannot be determined radiologically and during extraction of these teeth for prophylactic purposes.</description><subject>Dental Sac - metabolism</subject><subject>Dentistry</subject><subject>Humans</subject><subject>Medicine</subject><subject>Molar, Third - pathology</subject><subject>Original Article</subject><subject>Osteogenesis</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Tooth, Impacted - diagnostic imaging</subject><subject>Tooth, Impacted - genetics</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtO5DAQRS0EGl7zAyxQlmzM-JU4WSI0PCQE0gjWlmNXGiPHbuykRf_NfAtfNm4aZsnGvqq6daV7EDqh5JwSIn_l8rQCE0YxEazu8HoHHVDBG8ylpLsfmuGma-k-Osz5hRAqGsl_oH0uurZtOn6AVrdhBXlyCz25GKo4VNMzvP-Ft2WCnDcjDyvwm4WPYVGFGLCJ1hX55_4iVy5UFsKkfTVE753xkDdeNy61mcBWow7W9bPXqQS7VAax6HyM9gbtM_z8_I_Q09Xvx8sbfPdwfXt5cYcNF3LCvR4II7rVQA2Tg7ECRN1YyaHphrrrGBO65b2RtWa0VLNGt7Zu-r4UlEZafoTOtrnLFF_nUlSNLhvwXgeIc1asoZLJtuayWNnWalLMOcGglsmNOq0VJWrDW215q8JbffBW63J0-pk_9yPY_ydfgIuBbw25rMICknqJcwql83ex_wD24I7j</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Ege, Bilal</creator><creator>Koparal, Mahmut</creator><creator>Kurt, Muhammed Yusuf</creator><creator>Bozgeyik, Esra</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1817-1230</orcidid><orcidid>https://orcid.org/0000-0002-1360-276X</orcidid><orcidid>https://orcid.org/0000-0002-8726-3182</orcidid><orcidid>https://orcid.org/0000-0002-1279-0893</orcidid></search><sort><creationdate>20220301</creationdate><title>Investigation of the expression level of long non-coding RNAs in dental follicles of impacted mandibular third molars</title><author>Ege, Bilal ; Koparal, Mahmut ; Kurt, Muhammed Yusuf ; Bozgeyik, Esra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-baf020a8ae1c27fcd4e456d73e69f599224a83bc75a21673dca8d56bb3497c7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Dental Sac - metabolism</topic><topic>Dentistry</topic><topic>Humans</topic><topic>Medicine</topic><topic>Molar, Third - pathology</topic><topic>Original Article</topic><topic>Osteogenesis</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Tooth, Impacted - diagnostic imaging</topic><topic>Tooth, Impacted - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ege, Bilal</creatorcontrib><creatorcontrib>Koparal, Mahmut</creatorcontrib><creatorcontrib>Kurt, Muhammed Yusuf</creatorcontrib><creatorcontrib>Bozgeyik, Esra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ege, Bilal</au><au>Koparal, Mahmut</au><au>Kurt, Muhammed Yusuf</au><au>Bozgeyik, Esra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the expression level of long non-coding RNAs in dental follicles of impacted mandibular third molars</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>26</volume><issue>3</issue><spage>2817</spage><epage>2825</epage><pages>2817-2825</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
Dental follicle (DF) is made up of mesenchymal cells and fibers surrounding the enamel organ of a developing tooth. It has been shown that cystic and neoplastic lesions can develop from the pericoronal follicles of impacted third molars (ITMs). But the molecular transformation of DF tissues has not yet been uncovered and remains elusive. Accordingly, in the present study, we aimed to investigate the differential expression of lncRNA genes in DF tissues associated with asymptomatic impacted mandibular third molars (IMTMs) that do not show pathological pericoronal radiolucency in radiographic examination.
Material and methods
A total of 30 patients with unilateral mesioangular IMTMs were enrolled for the study. The expressions of lncRNA genes were determined in the DF and healthy gingival tissues obtained from study patients. For the determination of lncRNA expression levels, RNA was isolated from the obtained tissues, converted to cDNA samples, and analyzed by quantitative real-time PCR method.
Results
As a result, we found that the gene expression of MEG3 was increased about 10-fold in DF tissues compared to healthy gingival tissues (
p
< 0.0001). In addition, NORAD expression was found to be upregulated 4.2-fold (
p
= 0.0002) in DF tissues. Also, expression level of MALAT1 was found to be decreased 1.24-fold (
p
= 0.584) and TP73-AS1 increased 2.6-fold (
p
= 0.093) in DF tissues compared to healthy gingival tissues.
Conclusions
Consequently, present findings suggest that differentially expressed lncRNAs in DFs might be associated with the various levels of cellular events including osteogenic differentiation, DNA damage, and the transformation into odontogenic pathology.
Clinical relevance
Expression levels of MEG3 and NORAD lncRNA molecules may guide clinicians in the evaluation of asymptomatic ITM dental follicles that cannot be determined radiologically and during extraction of these teeth for prophylactic purposes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34988693</pmid><doi>10.1007/s00784-021-04259-y</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1817-1230</orcidid><orcidid>https://orcid.org/0000-0002-1360-276X</orcidid><orcidid>https://orcid.org/0000-0002-8726-3182</orcidid><orcidid>https://orcid.org/0000-0002-1279-0893</orcidid></addata></record> |
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| subjects | Dental Sac - metabolism Dentistry Humans Medicine Molar, Third - pathology Original Article Osteogenesis RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Tooth, Impacted - diagnostic imaging Tooth, Impacted - genetics |
| title | Investigation of the expression level of long non-coding RNAs in dental follicles of impacted mandibular third molars |
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