Characterization, genome analysis and in vitro activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant and extensively drug‐resistant biofilm‐forming uropathogenic Escherichia coli isolates, India
Aim We aimed to study host range, stability, genome and antibiofilm activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant (MDR) and extensively drug‐resistant (XDR) biofilm‐forming uropathogenic Escherichia coli isolates. Methods and Results A novel lytic phage vB_EcoA_RDN8.1...
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| Veröffentlicht in: | Journal of applied microbiology 2022-04, Vol.132 (4), p.3387-3404 |
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| container_title | Journal of applied microbiology |
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| creator | Chaudhary, Naveen Mohan, Balvinder Mavuduru, Ravimohan S. Kumar, Yashwant Taneja, Neelam |
| description | Aim
We aimed to study host range, stability, genome and antibiofilm activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant (MDR) and extensively drug‐resistant (XDR) biofilm‐forming uropathogenic Escherichia coli isolates.
Methods and Results
A novel lytic phage vB_EcoA_RDN8.1 active against UPEC strains resistant to third‐generation cephalosporins, fluoroquinolones, aminoglycosides, imipenem, beta‐lactamase inhibitor combination and polymyxins was isolated from community raw sewage water of Chandigarh. It exhibited a clear plaque morphology and a burst size of 250. In the time‐kill assay, the maximum amount of killing was achieved at MOI 1.0. vB_EcoA_RDN8.1 belongs to the family Autographiviridae, has a genome size of 39.5 kb with a GC content of 51.6%. It was stable over a wide range of temperatures and pH. It was able to inhibit biofilm formation which may be related to an endolysin encoded by ORF 19.
Conclusions
The vB_EcoA_RDN8.1 is a novel lytic phage that has the potential for inclusion into phage cocktails being developed for the treatment of urinary tract infections (UTIs) caused by highly drug‐resistant UPEC.
Significance and Impact of the Study
We provide a detailed characterization of a novel lytic Escherichia phage with antibiofilm activity having a potential application against MDR and XDR UPEC causing UTIs. |
| doi_str_mv | 10.1111/jam.15439 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2617275229</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2644357512</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-5d96c69d500a982e96855c833ac314478c9590ac83c856ff5a586bed49e5a9893</originalsourceid><addsrcrecordid>eNp1kd-K1DAUh4so7h-98AUk4I3CdrZpmra5HMdRV1YF0etwJk3bDGkyJum49cpH8A0Fn8TMdFUQzE0OJ18-DueXJI9wtsDxXG5hWGBaEHYnOcWkpGleVvndY12kNKvyk-TM-22WYZLR8n5yQgpWs6yip8mPVQ8ORJBOfYWgrLlAnTR2kAgM6MkrH4sGKYP2KjiLIqpiNSHbIkDG7qVGux46ifbP-VrYJf_w4l29wDMYLR0o4wMaRh3Uz2_fGzd2yMnoDWDC0S1vgjQ-wnpCh-dI_QU2yrZKD7HXWjco06HR2R2E3sYxlUBrL_o4u-gVIGG1QspbDUH6C3RlGgUPknstaC8f3t7nyaeX64-r1-n1-1dXq-V1KgglLKUNK0XJGpplwOpcsrKmVNSEgCC4KKpaMMoyiB1R07JtKdC63MimYJLGD4ycJ09n787Zz6P0gQ_KC6k1GGlHz_MSV3lF8_yAPvkH3drRxW0fqKIgtKI4j9SzmRLOeu9ky3dODeAmjjN-SJ3H1Pkx9cg-vjWOm0E2f8jfMUfgcga-KC2n_5v4m-XbWfkL6z-9vA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2644357512</pqid></control><display><type>article</type><title>Characterization, genome analysis and in vitro activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant and extensively drug‐resistant biofilm‐forming uropathogenic Escherichia coli isolates, India</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Chaudhary, Naveen ; Mohan, Balvinder ; Mavuduru, Ravimohan S. ; Kumar, Yashwant ; Taneja, Neelam</creator><creatorcontrib>Chaudhary, Naveen ; Mohan, Balvinder ; Mavuduru, Ravimohan S. ; Kumar, Yashwant ; Taneja, Neelam</creatorcontrib><description>Aim
We aimed to study host range, stability, genome and antibiofilm activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant (MDR) and extensively drug‐resistant (XDR) biofilm‐forming uropathogenic Escherichia coli isolates.
Methods and Results
A novel lytic phage vB_EcoA_RDN8.1 active against UPEC strains resistant to third‐generation cephalosporins, fluoroquinolones, aminoglycosides, imipenem, beta‐lactamase inhibitor combination and polymyxins was isolated from community raw sewage water of Chandigarh. It exhibited a clear plaque morphology and a burst size of 250. In the time‐kill assay, the maximum amount of killing was achieved at MOI 1.0. vB_EcoA_RDN8.1 belongs to the family Autographiviridae, has a genome size of 39.5 kb with a GC content of 51.6%. It was stable over a wide range of temperatures and pH. It was able to inhibit biofilm formation which may be related to an endolysin encoded by ORF 19.
Conclusions
The vB_EcoA_RDN8.1 is a novel lytic phage that has the potential for inclusion into phage cocktails being developed for the treatment of urinary tract infections (UTIs) caused by highly drug‐resistant UPEC.
Significance and Impact of the Study
We provide a detailed characterization of a novel lytic Escherichia phage with antibiofilm activity having a potential application against MDR and XDR UPEC causing UTIs.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15439</identifier><identifier>PMID: 34989075</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Aminoglycosides ; Bacteriophages - genetics ; Biofilms ; Burst size ; Cephalosporins ; Drug resistance ; E coli ; Escherichia coli ; Fluoroquinolones ; Genomes ; Host range ; Humans ; Imipenem ; multidrug‐resistance ; Myoviridae ; Open reading frames ; phage cocktail ; phage therapy ; Phages ; Polymyxins ; Raw sewage ; Sewage ; Urinary tract ; Urinary Tract Infections ; uropathogenic Escherichia coli ; Uropathogenic Escherichia coli - genetics</subject><ispartof>Journal of applied microbiology, 2022-04, Vol.132 (4), p.3387-3404</ispartof><rights>2022 The Society for Applied Microbiology</rights><rights>2022 The Society for Applied Microbiology.</rights><rights>Copyright © 2022 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-5d96c69d500a982e96855c833ac314478c9590ac83c856ff5a586bed49e5a9893</citedby><cites>FETCH-LOGICAL-c3539-5d96c69d500a982e96855c833ac314478c9590ac83c856ff5a586bed49e5a9893</cites><orcidid>0000-0003-1198-6138</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.15439$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.15439$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34989075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaudhary, Naveen</creatorcontrib><creatorcontrib>Mohan, Balvinder</creatorcontrib><creatorcontrib>Mavuduru, Ravimohan S.</creatorcontrib><creatorcontrib>Kumar, Yashwant</creatorcontrib><creatorcontrib>Taneja, Neelam</creatorcontrib><title>Characterization, genome analysis and in vitro activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant and extensively drug‐resistant biofilm‐forming uropathogenic Escherichia coli isolates, India</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
We aimed to study host range, stability, genome and antibiofilm activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant (MDR) and extensively drug‐resistant (XDR) biofilm‐forming uropathogenic Escherichia coli isolates.
Methods and Results
A novel lytic phage vB_EcoA_RDN8.1 active against UPEC strains resistant to third‐generation cephalosporins, fluoroquinolones, aminoglycosides, imipenem, beta‐lactamase inhibitor combination and polymyxins was isolated from community raw sewage water of Chandigarh. It exhibited a clear plaque morphology and a burst size of 250. In the time‐kill assay, the maximum amount of killing was achieved at MOI 1.0. vB_EcoA_RDN8.1 belongs to the family Autographiviridae, has a genome size of 39.5 kb with a GC content of 51.6%. It was stable over a wide range of temperatures and pH. It was able to inhibit biofilm formation which may be related to an endolysin encoded by ORF 19.
Conclusions
The vB_EcoA_RDN8.1 is a novel lytic phage that has the potential for inclusion into phage cocktails being developed for the treatment of urinary tract infections (UTIs) caused by highly drug‐resistant UPEC.
Significance and Impact of the Study
We provide a detailed characterization of a novel lytic Escherichia phage with antibiofilm activity having a potential application against MDR and XDR UPEC causing UTIs.</description><subject>Aminoglycosides</subject><subject>Bacteriophages - genetics</subject><subject>Biofilms</subject><subject>Burst size</subject><subject>Cephalosporins</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Fluoroquinolones</subject><subject>Genomes</subject><subject>Host range</subject><subject>Humans</subject><subject>Imipenem</subject><subject>multidrug‐resistance</subject><subject>Myoviridae</subject><subject>Open reading frames</subject><subject>phage cocktail</subject><subject>phage therapy</subject><subject>Phages</subject><subject>Polymyxins</subject><subject>Raw sewage</subject><subject>Sewage</subject><subject>Urinary tract</subject><subject>Urinary Tract Infections</subject><subject>uropathogenic Escherichia coli</subject><subject>Uropathogenic Escherichia coli - genetics</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd-K1DAUh4so7h-98AUk4I3CdrZpmra5HMdRV1YF0etwJk3bDGkyJum49cpH8A0Fn8TMdFUQzE0OJ18-DueXJI9wtsDxXG5hWGBaEHYnOcWkpGleVvndY12kNKvyk-TM-22WYZLR8n5yQgpWs6yip8mPVQ8ORJBOfYWgrLlAnTR2kAgM6MkrH4sGKYP2KjiLIqpiNSHbIkDG7qVGux46ifbP-VrYJf_w4l29wDMYLR0o4wMaRh3Uz2_fGzd2yMnoDWDC0S1vgjQ-wnpCh-dI_QU2yrZKD7HXWjco06HR2R2E3sYxlUBrL_o4u-gVIGG1QspbDUH6C3RlGgUPknstaC8f3t7nyaeX64-r1-n1-1dXq-V1KgglLKUNK0XJGpplwOpcsrKmVNSEgCC4KKpaMMoyiB1R07JtKdC63MimYJLGD4ycJ09n787Zz6P0gQ_KC6k1GGlHz_MSV3lF8_yAPvkH3drRxW0fqKIgtKI4j9SzmRLOeu9ky3dODeAmjjN-SJ3H1Pkx9cg-vjWOm0E2f8jfMUfgcga-KC2n_5v4m-XbWfkL6z-9vA</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Chaudhary, Naveen</creator><creator>Mohan, Balvinder</creator><creator>Mavuduru, Ravimohan S.</creator><creator>Kumar, Yashwant</creator><creator>Taneja, Neelam</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1198-6138</orcidid></search><sort><creationdate>202204</creationdate><title>Characterization, genome analysis and in vitro activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant and extensively drug‐resistant biofilm‐forming uropathogenic Escherichia coli isolates, India</title><author>Chaudhary, Naveen ; Mohan, Balvinder ; Mavuduru, Ravimohan S. ; Kumar, Yashwant ; Taneja, Neelam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-5d96c69d500a982e96855c833ac314478c9590ac83c856ff5a586bed49e5a9893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aminoglycosides</topic><topic>Bacteriophages - genetics</topic><topic>Biofilms</topic><topic>Burst size</topic><topic>Cephalosporins</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Fluoroquinolones</topic><topic>Genomes</topic><topic>Host range</topic><topic>Humans</topic><topic>Imipenem</topic><topic>multidrug‐resistance</topic><topic>Myoviridae</topic><topic>Open reading frames</topic><topic>phage cocktail</topic><topic>phage therapy</topic><topic>Phages</topic><topic>Polymyxins</topic><topic>Raw sewage</topic><topic>Sewage</topic><topic>Urinary tract</topic><topic>Urinary Tract Infections</topic><topic>uropathogenic Escherichia coli</topic><topic>Uropathogenic Escherichia coli - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaudhary, Naveen</creatorcontrib><creatorcontrib>Mohan, Balvinder</creatorcontrib><creatorcontrib>Mavuduru, Ravimohan S.</creatorcontrib><creatorcontrib>Kumar, Yashwant</creatorcontrib><creatorcontrib>Taneja, Neelam</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaudhary, Naveen</au><au>Mohan, Balvinder</au><au>Mavuduru, Ravimohan S.</au><au>Kumar, Yashwant</au><au>Taneja, Neelam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization, genome analysis and in vitro activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant and extensively drug‐resistant biofilm‐forming uropathogenic Escherichia coli isolates, India</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2022-04</date><risdate>2022</risdate><volume>132</volume><issue>4</issue><spage>3387</spage><epage>3404</epage><pages>3387-3404</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
We aimed to study host range, stability, genome and antibiofilm activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant (MDR) and extensively drug‐resistant (XDR) biofilm‐forming uropathogenic Escherichia coli isolates.
Methods and Results
A novel lytic phage vB_EcoA_RDN8.1 active against UPEC strains resistant to third‐generation cephalosporins, fluoroquinolones, aminoglycosides, imipenem, beta‐lactamase inhibitor combination and polymyxins was isolated from community raw sewage water of Chandigarh. It exhibited a clear plaque morphology and a burst size of 250. In the time‐kill assay, the maximum amount of killing was achieved at MOI 1.0. vB_EcoA_RDN8.1 belongs to the family Autographiviridae, has a genome size of 39.5 kb with a GC content of 51.6%. It was stable over a wide range of temperatures and pH. It was able to inhibit biofilm formation which may be related to an endolysin encoded by ORF 19.
Conclusions
The vB_EcoA_RDN8.1 is a novel lytic phage that has the potential for inclusion into phage cocktails being developed for the treatment of urinary tract infections (UTIs) caused by highly drug‐resistant UPEC.
Significance and Impact of the Study
We provide a detailed characterization of a novel lytic Escherichia phage with antibiofilm activity having a potential application against MDR and XDR UPEC causing UTIs.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>34989075</pmid><doi>10.1111/jam.15439</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0003-1198-6138</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2022-04, Vol.132 (4), p.3387-3404 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Aminoglycosides Bacteriophages - genetics Biofilms Burst size Cephalosporins Drug resistance E coli Escherichia coli Fluoroquinolones Genomes Host range Humans Imipenem multidrug‐resistance Myoviridae Open reading frames phage cocktail phage therapy Phages Polymyxins Raw sewage Sewage Urinary tract Urinary Tract Infections uropathogenic Escherichia coli Uropathogenic Escherichia coli - genetics |
| title | Characterization, genome analysis and in vitro activity of a novel phage vB_EcoA_RDN8.1 active against multi‐drug resistant and extensively drug‐resistant biofilm‐forming uropathogenic Escherichia coli isolates, India |
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