Self-assembled chitosan derived microparticles inhibit tumor angiogenesis and induce apoptosis in Ehrlich-ascites-tumor bearing mice
Self-assembled microparticles from chitosan (SAMC) was prepared by depolymerization induced by potassium persulfate. Particle size distribution data showed averaged around 5 μm size and SEM indicated the sequential formation of “RBC” shaped particles. Soluble SAMC consists of ‘deacetylated’ residues...
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| Veröffentlicht in: | Carbohydrate polymers 2022-02, Vol.278, p.118941-118941, Article 118941 |
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| creator | Punarvasu, T.P. Prashanth, K.V. Harish |
| description | Self-assembled microparticles from chitosan (SAMC) was prepared by depolymerization induced by potassium persulfate. Particle size distribution data showed averaged around 5 μm size and SEM indicated the sequential formation of “RBC” shaped particles. Soluble SAMC consists of ‘deacetylated’ residues as revealed by 13C NMR. SAMC showed antitumor efficacy in human breast cancer cell lines through mitigation in cell proliferation, colony formation and cell migration. Anti-tumor and anti-angiogenic properties of SAMC was found in vivo Ehrlich ascites tumor (EAT) bearing mice model resulting in tumor growth inhibition (EAT control, 17.4 ml; SAMC treated, 6.8 ml) and improved survival potency (15 days). Moreover, the decrease in ascites VEGF secretion (EAT control, 1354 ng; SAMC treated, 351 ng) accompanied with reduction in neovessel formation. Apoptosis induction by SAMC was confirmed by DNA fragmentation, caspase activities and fluorescence staining methods respectively. SAMC may be a safe candidate for anti-tumor dietary supplement production in food industry.
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| doi_str_mv | 10.1016/j.carbpol.2021.118941 |
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[Display omitted]</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Carbohydrate Conformation</subject><subject>Carcinoma, Ehrlich Tumor - drug therapy</subject><subject>Carcinoma, Ehrlich Tumor - metabolism</subject><subject>Carcinoma, Ehrlich Tumor - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Chitosan</subject><subject>Chitosan - chemical synthesis</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>Cytotoxicity</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Ehrlich ascites tumor</subject><subject>Female</subject><subject>Humans</subject><subject>Mice</subject><subject>Microparticles</subject><subject>Neoplasms, Experimental - drug therapy</subject><subject>Neoplasms, Experimental - metabolism</subject><subject>Neoplasms, Experimental - pathology</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Neovascularization, Pathologic - pathology</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtP3DAQtqqiskB_Qqsce8niR-w4p6pCtCAhcQDOlmNPdmeVxKmdIHHvD69XWXrtXKwZfw_NfIR8YXTLKFPXh62zsZ1Cv-WUsy1juqnYB7Jhum5KJqrqI9lQVlWlVqw-JxcpHWguxegnci6qpha1bDbkzxP0XWlTgqHtwRduj3NIdiw8RHzNgwFdDJONM7oeUoHjHluci3kZQizsuMOwgxESptz4_O0XB4WdwpRl8IgvbvexR7fPJg5nSOVKbcFGHHdHfbgiZ53tE3w-vZfk5eft881d-fD46_7mx0PphJJzKVTrpLQgwXJvqWqbroFKc9HIjmvJaidbaTtBVVPX3HVaKa2tdYpK0SnuxSX5tupOMfxeIM1mwOSg7-0IYUmGK6a41lKzDJUrNG-fUoTOTBEHG98Mo-YYgDmYUwDmGIBZA8i8ryeLpR3A_2O9XzwDvq8AyIu-IkSTzwKjA48R3Gx8wP9Y_AURS5yw</recordid><startdate>20220215</startdate><enddate>20220215</enddate><creator>Punarvasu, T.P.</creator><creator>Prashanth, K.V. Harish</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220215</creationdate><title>Self-assembled chitosan derived microparticles inhibit tumor angiogenesis and induce apoptosis in Ehrlich-ascites-tumor bearing mice</title><author>Punarvasu, T.P. ; Prashanth, K.V. Harish</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-36bc55ae5ea2da06b9f9e482395f28517c5b5af3069772cf86688aac6053f62d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Carbohydrate Conformation</topic><topic>Carcinoma, Ehrlich Tumor - drug therapy</topic><topic>Carcinoma, Ehrlich Tumor - metabolism</topic><topic>Carcinoma, Ehrlich Tumor - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Chitosan</topic><topic>Chitosan - chemical synthesis</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - pharmacology</topic><topic>Cytotoxicity</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Ehrlich ascites tumor</topic><topic>Female</topic><topic>Humans</topic><topic>Mice</topic><topic>Microparticles</topic><topic>Neoplasms, Experimental - drug therapy</topic><topic>Neoplasms, Experimental - metabolism</topic><topic>Neoplasms, Experimental - pathology</topic><topic>Neovascularization, Pathologic - drug therapy</topic><topic>Neovascularization, Pathologic - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Punarvasu, T.P.</creatorcontrib><creatorcontrib>Prashanth, K.V. 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Harish</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Self-assembled chitosan derived microparticles inhibit tumor angiogenesis and induce apoptosis in Ehrlich-ascites-tumor bearing mice</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2022-02-15</date><risdate>2022</risdate><volume>278</volume><spage>118941</spage><epage>118941</epage><pages>118941-118941</pages><artnum>118941</artnum><issn>0144-8617</issn><eissn>1879-1344</eissn><abstract>Self-assembled microparticles from chitosan (SAMC) was prepared by depolymerization induced by potassium persulfate. Particle size distribution data showed averaged around 5 μm size and SEM indicated the sequential formation of “RBC” shaped particles. Soluble SAMC consists of ‘deacetylated’ residues as revealed by 13C NMR. SAMC showed antitumor efficacy in human breast cancer cell lines through mitigation in cell proliferation, colony formation and cell migration. Anti-tumor and anti-angiogenic properties of SAMC was found in vivo Ehrlich ascites tumor (EAT) bearing mice model resulting in tumor growth inhibition (EAT control, 17.4 ml; SAMC treated, 6.8 ml) and improved survival potency (15 days). Moreover, the decrease in ascites VEGF secretion (EAT control, 1354 ng; SAMC treated, 351 ng) accompanied with reduction in neovessel formation. Apoptosis induction by SAMC was confirmed by DNA fragmentation, caspase activities and fluorescence staining methods respectively. SAMC may be a safe candidate for anti-tumor dietary supplement production in food industry.
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| subjects | Angiogenesis Animals Apoptosis Apoptosis - drug effects Carbohydrate Conformation Carcinoma, Ehrlich Tumor - drug therapy Carcinoma, Ehrlich Tumor - metabolism Carcinoma, Ehrlich Tumor - pathology Cell Line, Tumor Cell Movement - drug effects Cell Proliferation - drug effects Chitosan Chitosan - chemical synthesis Chitosan - chemistry Chitosan - pharmacology Cytotoxicity Drug Screening Assays, Antitumor Ehrlich ascites tumor Female Humans Mice Microparticles Neoplasms, Experimental - drug therapy Neoplasms, Experimental - metabolism Neoplasms, Experimental - pathology Neovascularization, Pathologic - drug therapy Neovascularization, Pathologic - pathology |
| title | Self-assembled chitosan derived microparticles inhibit tumor angiogenesis and induce apoptosis in Ehrlich-ascites-tumor bearing mice |
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