N6-Methyladenosine Reader YTHDF1 Promotes ARHGEF2 Translation and RhoA Signaling in Colorectal Cancer
N6-methyladenosine (m6A) governs the fate of RNAs through m6A readers. Colorectal cancer (CRC) exhibits aberrant m6A modifications and expression of m6A regulators. However, how m6A readers interpret oncogenic m6A methylome to promote malignant transformation remains to be illustrated. YTH N6-methyl...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 2022-04, Vol.162 (4), p.1183-1196 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
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| creator | Wang, Shiyan Gao, Shanshan Zeng, Yong Zhu, Lin Mo, Yulin Wong, Chi Chun Bao, Yi Su, Peiran Zhai, Jianning Wang, Lina Soares, Fraser Xu, Xin Chen, Huarong Hezaveh, Kebria Ci, Xinpei He, Aobo McGaha, Tracy O’Brien, Catherine Rottapel, Robert Kang, Wei Wu, Jianfeng Zheng, Gang Cai, Zongwei Yu, Jun He, Housheng Hansen |
| description | N6-methyladenosine (m6A) governs the fate of RNAs through m6A readers. Colorectal cancer (CRC) exhibits aberrant m6A modifications and expression of m6A regulators. However, how m6A readers interpret oncogenic m6A methylome to promote malignant transformation remains to be illustrated.
YTH N6-methyladenosine RNA binding protein 1 (Ythdf1) knockout mouse was generated to determine the effect of Ythdf1 in CRC tumorigenesis in vivo. Multiomic analysis of RNA-sequencing, m6A methylated RNA immunoprecipitation sequencing, YTHDF1 RNA immunoprecipitation sequencing, and proteomics were performed to unravel targets of YTHDF1 in CRC. The therapeutic potential of targeting YTHDF1-m6A-Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2) was evaluated using small interfering RNA (siRNA) encapsulated by lipid nanoparticles (LNP).
DNA copy number gain of YTHDF1 is a frequent event in CRC and contributes to its overexpression. High expression of YTHDF1 is significantly associated with metastatic gene signature in patient tumors. Ythdf1 knockout in mice dampened tumor growth in an inflammatory CRC model. YTHDF1 promotes cell growth in CRC cell lines and primary organoids and lung and liver metastasis in vivo. Integrative multiomics analysis identified RhoA activator ARHGEF2 as a key downstream target of YTHDF1. YTHDF1 binds to m6A sites of ARHGEF2 messenger RNA, resulting in enhanced translation of ARHGEF2. Ectopic expression of ARHGEF2 restored impaired RhoA signaling, cell growth, and metastatic ability both in vitro and in vivo caused by YTHDF1 loss, verifying that ARHGEF2 is a key target of YTHDF1. Finally, ARHGEF2 siRNA delivered by LNP significantly suppressed tumor growth and metastasis in vivo.
We identify a novel oncogenic epitranscriptome axis of YTHDF1-m6A-ARHGEF2, which regulates CRC tumorigenesis and metastasis. siRNA-delivering LNP drug validated the therapeutic potential of targeting this axis in CRC.
[Display omitted]
N6-methyladenosine reader YTHDF1 is amplified and overexpressed in colorectal cancer. YTHDF1-m6A-ARHGEF2 axis contributes to tumorigenesis and metastasis in colorectal cancer and is a potential therapeutic target. |
| doi_str_mv | 10.1053/j.gastro.2021.12.269 |
| format | Article |
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YTH N6-methyladenosine RNA binding protein 1 (Ythdf1) knockout mouse was generated to determine the effect of Ythdf1 in CRC tumorigenesis in vivo. Multiomic analysis of RNA-sequencing, m6A methylated RNA immunoprecipitation sequencing, YTHDF1 RNA immunoprecipitation sequencing, and proteomics were performed to unravel targets of YTHDF1 in CRC. The therapeutic potential of targeting YTHDF1-m6A-Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2) was evaluated using small interfering RNA (siRNA) encapsulated by lipid nanoparticles (LNP).
DNA copy number gain of YTHDF1 is a frequent event in CRC and contributes to its overexpression. High expression of YTHDF1 is significantly associated with metastatic gene signature in patient tumors. Ythdf1 knockout in mice dampened tumor growth in an inflammatory CRC model. YTHDF1 promotes cell growth in CRC cell lines and primary organoids and lung and liver metastasis in vivo. Integrative multiomics analysis identified RhoA activator ARHGEF2 as a key downstream target of YTHDF1. YTHDF1 binds to m6A sites of ARHGEF2 messenger RNA, resulting in enhanced translation of ARHGEF2. Ectopic expression of ARHGEF2 restored impaired RhoA signaling, cell growth, and metastatic ability both in vitro and in vivo caused by YTHDF1 loss, verifying that ARHGEF2 is a key target of YTHDF1. Finally, ARHGEF2 siRNA delivered by LNP significantly suppressed tumor growth and metastasis in vivo.
We identify a novel oncogenic epitranscriptome axis of YTHDF1-m6A-ARHGEF2, which regulates CRC tumorigenesis and metastasis. siRNA-delivering LNP drug validated the therapeutic potential of targeting this axis in CRC.
[Display omitted]
N6-methyladenosine reader YTHDF1 is amplified and overexpressed in colorectal cancer. YTHDF1-m6A-ARHGEF2 axis contributes to tumorigenesis and metastasis in colorectal cancer and is a potential therapeutic target.</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1053/j.gastro.2021.12.269</identifier><identifier>PMID: 34968454</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine - analogs & derivatives ; Adenosine - metabolism ; Animals ; ARHGEF2 ; Carcinogenesis - genetics ; Colorectal Cancer ; Colorectal Neoplasms - pathology ; Gene Expression Regulation, Neoplastic ; Humans ; Liposomes ; Mice ; N6-Methyladenosine ; Nanoparticle ; Nanoparticles ; Rho Guanine Nucleotide Exchange Factors - genetics ; Rho Guanine Nucleotide Exchange Factors - metabolism ; rhoA GTP-Binding Protein - genetics ; rhoA GTP-Binding Protein - metabolism ; RNA, Small Interfering ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; YTHDF1</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 2022-04, Vol.162 (4), p.1183-1196</ispartof><rights>2022 The Authors</rights><rights>Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-10eaf0dfe90981a67474fbef9e9afb34487223526c91a8cf4b2ad34b0b943ab93</citedby><cites>FETCH-LOGICAL-c408t-10eaf0dfe90981a67474fbef9e9afb34487223526c91a8cf4b2ad34b0b943ab93</cites><orcidid>0000-0003-2898-3363</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016508521041652$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34968454$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Shiyan</creatorcontrib><creatorcontrib>Gao, Shanshan</creatorcontrib><creatorcontrib>Zeng, Yong</creatorcontrib><creatorcontrib>Zhu, Lin</creatorcontrib><creatorcontrib>Mo, Yulin</creatorcontrib><creatorcontrib>Wong, Chi Chun</creatorcontrib><creatorcontrib>Bao, Yi</creatorcontrib><creatorcontrib>Su, Peiran</creatorcontrib><creatorcontrib>Zhai, Jianning</creatorcontrib><creatorcontrib>Wang, Lina</creatorcontrib><creatorcontrib>Soares, Fraser</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Chen, Huarong</creatorcontrib><creatorcontrib>Hezaveh, Kebria</creatorcontrib><creatorcontrib>Ci, Xinpei</creatorcontrib><creatorcontrib>He, Aobo</creatorcontrib><creatorcontrib>McGaha, Tracy</creatorcontrib><creatorcontrib>O’Brien, Catherine</creatorcontrib><creatorcontrib>Rottapel, Robert</creatorcontrib><creatorcontrib>Kang, Wei</creatorcontrib><creatorcontrib>Wu, Jianfeng</creatorcontrib><creatorcontrib>Zheng, Gang</creatorcontrib><creatorcontrib>Cai, Zongwei</creatorcontrib><creatorcontrib>Yu, Jun</creatorcontrib><creatorcontrib>He, Housheng Hansen</creatorcontrib><title>N6-Methyladenosine Reader YTHDF1 Promotes ARHGEF2 Translation and RhoA Signaling in Colorectal Cancer</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>N6-methyladenosine (m6A) governs the fate of RNAs through m6A readers. Colorectal cancer (CRC) exhibits aberrant m6A modifications and expression of m6A regulators. However, how m6A readers interpret oncogenic m6A methylome to promote malignant transformation remains to be illustrated.
YTH N6-methyladenosine RNA binding protein 1 (Ythdf1) knockout mouse was generated to determine the effect of Ythdf1 in CRC tumorigenesis in vivo. Multiomic analysis of RNA-sequencing, m6A methylated RNA immunoprecipitation sequencing, YTHDF1 RNA immunoprecipitation sequencing, and proteomics were performed to unravel targets of YTHDF1 in CRC. The therapeutic potential of targeting YTHDF1-m6A-Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2) was evaluated using small interfering RNA (siRNA) encapsulated by lipid nanoparticles (LNP).
DNA copy number gain of YTHDF1 is a frequent event in CRC and contributes to its overexpression. High expression of YTHDF1 is significantly associated with metastatic gene signature in patient tumors. Ythdf1 knockout in mice dampened tumor growth in an inflammatory CRC model. YTHDF1 promotes cell growth in CRC cell lines and primary organoids and lung and liver metastasis in vivo. Integrative multiomics analysis identified RhoA activator ARHGEF2 as a key downstream target of YTHDF1. YTHDF1 binds to m6A sites of ARHGEF2 messenger RNA, resulting in enhanced translation of ARHGEF2. Ectopic expression of ARHGEF2 restored impaired RhoA signaling, cell growth, and metastatic ability both in vitro and in vivo caused by YTHDF1 loss, verifying that ARHGEF2 is a key target of YTHDF1. Finally, ARHGEF2 siRNA delivered by LNP significantly suppressed tumor growth and metastasis in vivo.
We identify a novel oncogenic epitranscriptome axis of YTHDF1-m6A-ARHGEF2, which regulates CRC tumorigenesis and metastasis. siRNA-delivering LNP drug validated the therapeutic potential of targeting this axis in CRC.
[Display omitted]
N6-methyladenosine reader YTHDF1 is amplified and overexpressed in colorectal cancer. YTHDF1-m6A-ARHGEF2 axis contributes to tumorigenesis and metastasis in colorectal cancer and is a potential therapeutic target.</description><subject>Adenosine - analogs & derivatives</subject><subject>Adenosine - metabolism</subject><subject>Animals</subject><subject>ARHGEF2</subject><subject>Carcinogenesis - genetics</subject><subject>Colorectal Cancer</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Liposomes</subject><subject>Mice</subject><subject>N6-Methyladenosine</subject><subject>Nanoparticle</subject><subject>Nanoparticles</subject><subject>Rho Guanine Nucleotide Exchange Factors - genetics</subject><subject>Rho Guanine Nucleotide Exchange Factors - metabolism</subject><subject>rhoA GTP-Binding Protein - genetics</subject><subject>rhoA GTP-Binding Protein - metabolism</subject><subject>RNA, Small Interfering</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>YTHDF1</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EgvL4A4S8ZJPgV9J4g1QVSpF4qZQFK2uSTIqr1AY7ReLvSVVgyWpmce5czSHklLOUs0xeLNMFxC74VDDBUy5SkesdMuCZKBLGuNglg37kScaK7IAcxrhkjGlZ8H1yIJXOC5WpAcGHPLnH7u2rhRqdj9YhnWG_B_o6n15NOH0KfuU7jHQ0m95cTwSdB3Cxhc56R8HVdPbmR_TZLhy01i2odXTsWx-w6qClY3AVhmOy10Ab8eRnHpGXyfV8PE3uHm9ux6O7pFKs6BLOEBpWN6iZLjjkQzVUTYmNRg1NKZUqhkLITOSV5lBUjSoF1FKVrNRKQqnlETnf3n0P_mONsTMrGytsW3Do19GInGeaa6l4j6otWgUfY8DGvAe7gvBlODMbwWZptoLNRrDhok9vGs5-GtblCuu_0K_RHrjcAtj_-WkxmFhZ7CXUdqPE1N7-3_ANwouNXw</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Wang, Shiyan</creator><creator>Gao, Shanshan</creator><creator>Zeng, Yong</creator><creator>Zhu, Lin</creator><creator>Mo, Yulin</creator><creator>Wong, Chi Chun</creator><creator>Bao, Yi</creator><creator>Su, Peiran</creator><creator>Zhai, Jianning</creator><creator>Wang, Lina</creator><creator>Soares, Fraser</creator><creator>Xu, Xin</creator><creator>Chen, Huarong</creator><creator>Hezaveh, Kebria</creator><creator>Ci, Xinpei</creator><creator>He, Aobo</creator><creator>McGaha, Tracy</creator><creator>O’Brien, Catherine</creator><creator>Rottapel, Robert</creator><creator>Kang, Wei</creator><creator>Wu, Jianfeng</creator><creator>Zheng, Gang</creator><creator>Cai, Zongwei</creator><creator>Yu, Jun</creator><creator>He, Housheng Hansen</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2898-3363</orcidid></search><sort><creationdate>202204</creationdate><title>N6-Methyladenosine Reader YTHDF1 Promotes ARHGEF2 Translation and RhoA Signaling in Colorectal Cancer</title><author>Wang, Shiyan ; Gao, Shanshan ; Zeng, Yong ; Zhu, Lin ; Mo, Yulin ; Wong, Chi Chun ; Bao, Yi ; Su, Peiran ; Zhai, Jianning ; Wang, Lina ; Soares, Fraser ; Xu, Xin ; Chen, Huarong ; Hezaveh, Kebria ; Ci, Xinpei ; He, Aobo ; McGaha, Tracy ; O’Brien, Catherine ; Rottapel, Robert ; Kang, Wei ; Wu, Jianfeng ; Zheng, Gang ; Cai, Zongwei ; Yu, Jun ; He, Housheng Hansen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-10eaf0dfe90981a67474fbef9e9afb34487223526c91a8cf4b2ad34b0b943ab93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenosine - analogs & derivatives</topic><topic>Adenosine - metabolism</topic><topic>Animals</topic><topic>ARHGEF2</topic><topic>Carcinogenesis - genetics</topic><topic>Colorectal Cancer</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Liposomes</topic><topic>Mice</topic><topic>N6-Methyladenosine</topic><topic>Nanoparticle</topic><topic>Nanoparticles</topic><topic>Rho Guanine Nucleotide Exchange Factors - genetics</topic><topic>Rho Guanine Nucleotide Exchange Factors - metabolism</topic><topic>rhoA GTP-Binding Protein - genetics</topic><topic>rhoA GTP-Binding Protein - metabolism</topic><topic>RNA, Small Interfering</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>YTHDF1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Shiyan</creatorcontrib><creatorcontrib>Gao, Shanshan</creatorcontrib><creatorcontrib>Zeng, Yong</creatorcontrib><creatorcontrib>Zhu, Lin</creatorcontrib><creatorcontrib>Mo, Yulin</creatorcontrib><creatorcontrib>Wong, Chi Chun</creatorcontrib><creatorcontrib>Bao, Yi</creatorcontrib><creatorcontrib>Su, Peiran</creatorcontrib><creatorcontrib>Zhai, Jianning</creatorcontrib><creatorcontrib>Wang, Lina</creatorcontrib><creatorcontrib>Soares, Fraser</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Chen, Huarong</creatorcontrib><creatorcontrib>Hezaveh, Kebria</creatorcontrib><creatorcontrib>Ci, Xinpei</creatorcontrib><creatorcontrib>He, Aobo</creatorcontrib><creatorcontrib>McGaha, Tracy</creatorcontrib><creatorcontrib>O’Brien, Catherine</creatorcontrib><creatorcontrib>Rottapel, Robert</creatorcontrib><creatorcontrib>Kang, Wei</creatorcontrib><creatorcontrib>Wu, Jianfeng</creatorcontrib><creatorcontrib>Zheng, Gang</creatorcontrib><creatorcontrib>Cai, Zongwei</creatorcontrib><creatorcontrib>Yu, Jun</creatorcontrib><creatorcontrib>He, Housheng Hansen</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Shiyan</au><au>Gao, Shanshan</au><au>Zeng, Yong</au><au>Zhu, Lin</au><au>Mo, Yulin</au><au>Wong, Chi Chun</au><au>Bao, Yi</au><au>Su, Peiran</au><au>Zhai, Jianning</au><au>Wang, Lina</au><au>Soares, Fraser</au><au>Xu, Xin</au><au>Chen, Huarong</au><au>Hezaveh, Kebria</au><au>Ci, Xinpei</au><au>He, Aobo</au><au>McGaha, Tracy</au><au>O’Brien, Catherine</au><au>Rottapel, Robert</au><au>Kang, Wei</au><au>Wu, Jianfeng</au><au>Zheng, Gang</au><au>Cai, Zongwei</au><au>Yu, Jun</au><au>He, Housheng Hansen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N6-Methyladenosine Reader YTHDF1 Promotes ARHGEF2 Translation and RhoA Signaling in Colorectal Cancer</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>2022-04</date><risdate>2022</risdate><volume>162</volume><issue>4</issue><spage>1183</spage><epage>1196</epage><pages>1183-1196</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><abstract>N6-methyladenosine (m6A) governs the fate of RNAs through m6A readers. Colorectal cancer (CRC) exhibits aberrant m6A modifications and expression of m6A regulators. However, how m6A readers interpret oncogenic m6A methylome to promote malignant transformation remains to be illustrated.
YTH N6-methyladenosine RNA binding protein 1 (Ythdf1) knockout mouse was generated to determine the effect of Ythdf1 in CRC tumorigenesis in vivo. Multiomic analysis of RNA-sequencing, m6A methylated RNA immunoprecipitation sequencing, YTHDF1 RNA immunoprecipitation sequencing, and proteomics were performed to unravel targets of YTHDF1 in CRC. The therapeutic potential of targeting YTHDF1-m6A-Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2) was evaluated using small interfering RNA (siRNA) encapsulated by lipid nanoparticles (LNP).
DNA copy number gain of YTHDF1 is a frequent event in CRC and contributes to its overexpression. High expression of YTHDF1 is significantly associated with metastatic gene signature in patient tumors. Ythdf1 knockout in mice dampened tumor growth in an inflammatory CRC model. YTHDF1 promotes cell growth in CRC cell lines and primary organoids and lung and liver metastasis in vivo. Integrative multiomics analysis identified RhoA activator ARHGEF2 as a key downstream target of YTHDF1. YTHDF1 binds to m6A sites of ARHGEF2 messenger RNA, resulting in enhanced translation of ARHGEF2. Ectopic expression of ARHGEF2 restored impaired RhoA signaling, cell growth, and metastatic ability both in vitro and in vivo caused by YTHDF1 loss, verifying that ARHGEF2 is a key target of YTHDF1. Finally, ARHGEF2 siRNA delivered by LNP significantly suppressed tumor growth and metastasis in vivo.
We identify a novel oncogenic epitranscriptome axis of YTHDF1-m6A-ARHGEF2, which regulates CRC tumorigenesis and metastasis. siRNA-delivering LNP drug validated the therapeutic potential of targeting this axis in CRC.
[Display omitted]
N6-methyladenosine reader YTHDF1 is amplified and overexpressed in colorectal cancer. YTHDF1-m6A-ARHGEF2 axis contributes to tumorigenesis and metastasis in colorectal cancer and is a potential therapeutic target.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34968454</pmid><doi>10.1053/j.gastro.2021.12.269</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-2898-3363</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Gastroenterology (New York, N.Y. 1943), 2022-04, Vol.162 (4), p.1183-1196 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Alma/SFX Local Collection |
| subjects | Adenosine - analogs & derivatives Adenosine - metabolism Animals ARHGEF2 Carcinogenesis - genetics Colorectal Cancer Colorectal Neoplasms - pathology Gene Expression Regulation, Neoplastic Humans Liposomes Mice N6-Methyladenosine Nanoparticle Nanoparticles Rho Guanine Nucleotide Exchange Factors - genetics Rho Guanine Nucleotide Exchange Factors - metabolism rhoA GTP-Binding Protein - genetics rhoA GTP-Binding Protein - metabolism RNA, Small Interfering RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism YTHDF1 |
| title | N6-Methyladenosine Reader YTHDF1 Promotes ARHGEF2 Translation and RhoA Signaling in Colorectal Cancer |
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