Development of a high-throughput RT-PCR based viral infectivity assay for monitoring the stability of a replicating recombinant Lymphocytic Choriomeningitis viral vector

•Development of a RT-qPCR-based infectivity assay for a live, recombinant replicating lymphocytic choriomeningitis virus (LCMV) viral vector.•A high positive correlation between the RT-qPCR and the “gold-standard” infectivity assays.•The infectivity RT-qPCR assay demonstrated increased sample throug...

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Veröffentlicht in:	Journal of virological methods 2022-03, Vol.301, p.114440-114440, Article 114440
Hauptverfasser:	Gupta, Vineet, Antunez, Lorena R., Saleh-Birdjandi, Soraia, Kumru, Ozan S., Pospisil, Richard, Lilja, Anders, Fuhrmann, Gerhard, Smith, Lee, Volkin, David B., Joshi, Sangeeta B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Focus forming unit (FFU) Formulation Replicating recombinant lymphocytic choriomeningitis virus (TT1) Reverse transcription quantitative PCR (RT-qPCR) Stability Viral vector
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