Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Full-length huntingtin (FL HTT) is a large (aa 1-3,144), ubiquitously expressed, polyglutamine (polyQ)-containing protein with a mass of approximately 350 kDa. While the cellular function of FL HTT is not entirely understood, a mutant expansion of the polyQ tract above ~36 repeats is associated with...

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Veröffentlicht in:	Journal of visualized experiments 2021-12 (178)
Hauptverfasser:	Pace, James B, Huang, Nian N, Séguin, Jacqueline P, Esquina, Candi, Olin, Erin, Zhu, Guangyu, Carr, Grant
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blotting, Western Cell Line Humans Huntingtin Protein - genetics Huntingtin Protein - metabolism Huntington Disease - metabolism Mammals - metabolism Mutation Transfection
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